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Prh1 (NM_011174) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216719
Prh1 (tGFP-tagged) - Mouse proline rich protein HaeIII subfamily 1 (Prh1), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prh1
Synonyms A-ty; MP; MP2; Prp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216719 representing NM_011174, codon optimized.
Due to the complexity of NM_011174, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGTCGTCCTCTTCACAGTTGCCCTTCTCGCCCTGAGCAGTGCCCAAGAACCACGGGAAGAAAACC
AAAATCAGATTCAGATTCCTAACCAGCGCCCTCCACCCTCTGGGTTTCAGCCCCGACCACCTGTGAACGG
ATCTCAACAAGGCCCCCCTCCACCTGGCGGTCCACAGCCCAGACCTCCCCAAGGCCCCCCTCCTCCCGGC
GGACCACAACCTAGACCACCCCAGGGCCCACCTCCACCAGGAGTCCCACAACCACGACCCCCTCAGGGAC
CTCCACCTCCAGGCGGACCACAGCAGCGCCCTCCACAAGGTCCCCCCCCTCCTGGCGGACCCCAGCACCG
CCCCCCTCAAGGCCCCCCCCCACCAGGTGGACCACAGCAGCGACCACCCCAGGGTCCCCCACCACCAGGC
GGCCCCCAGCCCAGGCCCCCACAGGGTCCTCCACCCCCAGGAGGCCCTCAGCAACGACCGCCCCAGGGAC
CACCTCCACCAGGCGGCCCCCAACTTCGCCCCCCCCAAGGCCCCCCACCACCTGCCGGACCTCAACCACG
ACCACCTCAAGGACCTCCTCCTCCAGCCGGACCACAGCCCAGACCCCCTCAAGGACCTCCTACCACCGGC
CCTCAGCCAAGACCTACCCAGGGCCCACCACCAACTGGCGGACCTCAGCAACGACCACCTCAAGGACCCC
CACCACCTGGCGGCCCGCAGCCACGCCCTCCACAAGGCCCACCCCCCCCCGGAGGACCCCAACCATCACC
CACCCAAGGCCCCCCCCCAACAGGAGGACCTCAACAAACCCCCCCTCTGGCTGGCAACACTCAAGGCCCT
CCCCAGGGACGCCCACAAGGACCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216719 representing NM_011174
Red=Cloning site Green=Tags(s)

MLVVLFTVALLALSSAQEPREENQNQIQIPNQRPPPSGFQPRPPVNGSQQGPPPPGGPQPRPPQGPPPPG
GPQPRPPQGPPPPGVPQPRPPQGPPPPGGPQQRPPQGPPPPGGPQHRPPQGPPPPGGPQQRPPQGPPPPG
GPQPRPPQGPPPPGGPQQRPPQGPPPPGGPQLRPPQGPPPPAGPQPRPPQGPPPPAGPQPRPPQGPPTTG
PQPRPTQGPPPTGGPQQRPPQGPPPPGGPQPRPPQGPPPPGGPQPSPTQGPPPTGGPQQTPPLAGNTQGP
PQGRPQGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011174
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011174.4, NP_035304.4
RefSeq Size 870 bp
RefSeq ORF 870 bp
Locus ID 19131
Cytogenetics 6 G1
Summary This gene encodes a member of the heterogeneous family of proline-rich salivary glycoproteins. The expression of this gene is dramatically induced in the parotid and submandibular glands of mice by beta-adrenergic stimulation. The encoded protein serves an important dental function by protecting mice against toxic dietary polyphenols such as tannins and influence the visco-elastic properties of the mucus. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 6. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_011174" in other vectors (2)
SKU Description Size Price
MC209237 Prh1 (untagged) - Mouse proline rich protein HaeIII subfamily 1 (Prh1), (10ug) 10 ug
$330.00
MR216719 Prh1 (Myc-DDK-tagged) - Mouse proline rich protein HaeIII subfamily 1 (Prh1). Note: ORF is codon optimized 10 ug
$330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.