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Lmln (NM_172823) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216705
Lmln (tGFP-tagged) - Mouse leishmanolysin-like (metallopeptidase M8 family) (Lmln), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $825.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lmln
Synonyms 5330415H22Rik; AI465495
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216705 representing NM_172823
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGCTGGGTCTGGAGGAGCGGGCGGCCCGGGACCAGGGCCACGAGGCCGCTGGGGCGGCTGCC
TGTGGGTGCGAGGCGTCCTGCTTGTGCTGGGCGGGCTCCCGGCCGGTGCCGGAGCTGCTCCCGTCTCCCT
GGGCACTTCGCCTCCGTGTCGCCATCACGTCCTTTCGGACACTGAGGTCATAAATAAGGTCCATCTTAAG
ACAAATCATGTCACGAAGAGAGATGCTGACGGGCATTTACGAATCAAGACCATCTACGACCAGAGCATTG
AAGAGTTGCTCCCTGAGAAAAGATATCTTGTAAAGAACAAACTTTTCCCTCAAGCTATTTCTTATTTAGA
GAAAACATTTCAGGTTCGAAGACCTGCTGGCAGGATCTTACTTAGCAGACAATGTGCGACAAACCAGTAC
CTACGGAAGGAAAACGATCCACACAGATACTGTACTGGAGAGTGTGCGGTGCATACCAAGTGCGGGCCTG
TTATAGTGCCGGAGGAGCACCTCCAGCAATGCAGGGTCTGCCGAGAGGGTAAGTGGCCGTGTGGAGCAGT
GGGTGTGCTTGACCCAGAGGGTGTGCGGGACGCAGACTTCGTTCTTTATGTGGGTGCTCTGGCCACTGAG
AGGTGCAGCCACGAAAACATCATCTCCTACGCAGCCTATTGTCAGCAGGAGGCAAAAATGGACAGGCCCA
TAGCAGGATATGCTAACCTGTGTCCAAATATGATCTCCACCCAGCCCCAGGAGTTCATCGGGATGCTGTC
CACAGTGAAGCATGAGATCATTCATGCCCTGGGCTTCTCTGCTGGTCTTTTTGCATTCTACCATGATCAA
GATGGAAACCCCCTGACTTCAAGGTCTGCAGATGGCCTCCCGCCCTTCAACTATAGTCTTGGCTTGTATC
AGTGGAGTGATAAAGTGGTTCGGAAAGTGGAGAGATTGTGGAATGTTCGAGATAACAAGATAGTTCGCCA
CACTGTGTATCTCCTAGTAACACCGCGTGTTGTTGAAGAAGCTCGGAAGCATTTTAACTGTCCGGTCCTC
GAGGGCATGGAGCTTGAAAACCAAGGCGGGATGGGCACGGAGCTGAACCACTGGGAGAAGCGCTTGCTCG
AGAATGAAGCAATGACTGGTTCCCACACCCAGAACAGAGTCCTGTCTCGGATCACTCTGGCGTTAATGGA
GGACACTGGCTGGTACAAAGCCAATTACAGCATGGCTGAGAAACTCGACTGGGGCCGAGGACTGGGCTGT
GAATTTGTCAGGAAGAGCTGTAAATTCTGGATTGATCAGCACAGGCAAAGGAGGCAGGTGCCGAGCCCGT
ACTGTGACACACTCAGAAGTAACCCGCTGCAGCTGACCTGCAGACAGGACCAGAGAGCCGTCGCTGTGTG
TAACCTGCAGAGGTTTCCTAACCCCTTACCACCCGAGTACCAGTACTTTGATGAGCTCACTGGGATACCT
GCGGAGGATCTGCCTTACTATGGCGGGTCTGTGGAAATCGCTGACTATTGTCCTTTCAGTCAGGAATTCA
GCTGGCATTTAAGTGGCGAATACCAGCGCAGCTCCGACTGTAGAATCCTGGAAAACCAACCAGAGCTGTT
TAAGAACTACGGGGCAGAGCAGTACGGGCCGCACTCGGTCTGTCTGCTTCAGAAGTCTGCGTTCATCATG
GAGCAGTGCGAGAGGAAGCTCAGCTACCCAGACTGGGGGAGCGGCTGCTACCAGGTTTCTTGTTCTCCTC
AAGGTCTAAAGGTTTGGGTTCAAGACACTTCCTATCTGTGTAGCCGGGCCGGGCAGGTGCTCCCAGTCCG
AATTCAGATGAACGGCTGGATTCACAATGGAAACCTGCTGTGCCCTTCCTGCTGGGACTTCTGTGAGCAG
TGTCCACCAGAAACAGACCCTCCAGCTGCCAACCTGACTCGAGCCCTTCCTCTGGATCTCTGTTCCTGCT
CTTCTAGCCTGGTGGTCACACTTTGGCTCCTCCTCGGCAATCTGTTTCCTCTGCTGGCGGGGTTCCTTCT
GTGTGTGTGGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216705 representing NM_172823
Red=Cloning site Green=Tags(s)

MAAAGSGGAGGPGPGPRGRWGGCLWVRGVLLVLGGLPAGAGAAPVSLGTSPPCRHHVLSDTEVINKVHLK
TNHVTKRDADGHLRIKTIYDQSIEELLPEKRYLVKNKLFPQAISYLEKTFQVRRPAGRILLSRQCATNQY
LRKENDPHRYCTGECAVHTKCGPVIVPEEHLQQCRVCREGKWPCGAVGVLDPEGVRDADFVLYVGALATE
RCSHENIISYAAYCQQEAKMDRPIAGYANLCPNMISTQPQEFIGMLSTVKHEIIHALGFSAGLFAFYHDQ
DGNPLTSRSADGLPPFNYSLGLYQWSDKVVRKVERLWNVRDNKIVRHTVYLLVTPRVVEEARKHFNCPVL
EGMELENQGGMGTELNHWEKRLLENEAMTGSHTQNRVLSRITLALMEDTGWYKANYSMAEKLDWGRGLGC
EFVRKSCKFWIDQHRQRRQVPSPYCDTLRSNPLQLTCRQDQRAVAVCNLQRFPNPLPPEYQYFDELTGIP
AEDLPYYGGSVEIADYCPFSQEFSWHLSGEYQRSSDCRILENQPELFKNYGAEQYGPHSVCLLQKSAFIM
EQCERKLSYPDWGSGCYQVSCSPQGLKVWVQDTSYLCSRAGQVLPVRIQMNGWIHNGNLLCPSCWDFCEQ
CPPETDPPAANLTRALPLDLCSCSSSLVVTLWLLLGNLFPLLAGFLLCVWH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172823
ORF Size 2043 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172823.2, NP_766411.1
RefSeq Size 4111 bp
RefSeq ORF 2046 bp
Locus ID 239833
UniProt ID Q8BMN4
Cytogenetics 16 B3
Summary Metalloprotease.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172823" in other vectors (4)
SKU Description Size Price
MC220465 Lmln (untagged) - Mouse leishmanolysin-like (metallopeptidase M8 family) (Lmln), (10ug) 10 ug
$687.00
MR216705 Lmln (Myc-DDK-tagged) - Mouse leishmanolysin-like (metallopeptidase M8 family) (Lmln) 10 ug
$589.00 $625.00
MR216705L3 Lenti ORF clone of Lmln (Myc-DDK-tagged) - Mouse leishmanolysin-like (metallopeptidase M8 family) (Lmln) 10 ug
$925.00
MR216705L4 Lenti ORF clone of Lmln (mGFP-tagged) - Mouse leishmanolysin-like (metallopeptidase M8 family) (Lmln) 10 ug
$925.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.