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Dennd1b (NM_001166501) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216682
Dennd1b (tGFP-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$789.00 $901.00
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Other products for "Dennd1b or NM_001166501"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dennd1b
Synonyms 4632404N19Rik; 4930467M19Rik; 6820401H01Rik; F730008N07Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166501
ORF Size 2298 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166501.1, NP_001159973.1
RefSeq Size 8206 bp
RefSeq ORF 2301 bp
Locus ID 329260
UniProt ID Q3U1T9
Cytogenetics 1 E4
Summary Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001166501" in other vectors (4)
SKU Description Size Price
MC221470 Dennd1b (untagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1, (10ug) 10 ug
$772.00
MR216682 Dennd1b (Myc-DDK-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1 10 ug
$589.00 $701.00
MR216682L3 Lenti ORF clone of Dennd1b (Myc-DDK-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1 10 ug
$1,001.00
MR216682L4 Lenti ORF clone of Dennd1b (mGFP-tagged) - Mouse DENN/MADD domain containing 1B (Dennd1b), transcript variant 1 10 ug
$1,001.00

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