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Adam24 (NM_010086) Mouse Tagged ORF Clone

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SKU
MG216658
Adam24 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 24 (testase 1) (Adam24), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $897.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adam24
Synonyms Dtgn; Dtgn5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216658 representing NM_010086
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCTATGAGTGAGGCTCTGGTTCATGCAAGGATCACTCTCCTTCAGGCATGGTTGAGGATGCTGC
TCTTTTCTTCTGTATGGCCCCCGACTTGGTGTGCTGAATACAAAGGCCCTCCAGAGACAGTGAAACCCTT
GAGGGTAATTGTCTCTAGTAAAGACATGAGTCTTGCAGGCTGGATGTCCTATAGTCTGTACTTTGGAGGC
CAGAGACACATTATCTCCATGAAATCCAAGAATTTTTTGGAATCCAGACAGCTCCCTGTGTTCACATACA
ATGACCAAGGTGTCCTCTTTGAGGACAGACCTTTTGTCCAAAATGACTGTTATTATCTTGGTTTTGTGGA
TGGAGATCTAGAATCCATGGCTGCTCTTACCACCTGTTTTGGGGGCTTTCAAGGAATATTACAGATAAAT
GACACAGCTTATGAAATTAAGCCCAAGAGCCCCTCTTCCACATTTGAACATCTGCTTTACAAGCTAGACA
GTGAGAAAACTCAATTACGCCCCATGAGATGTGGATTAACAGATGAAGAAATAGAAGGGCAAGTGAGACT
TCAAGAAAATGGTAAGTCCACTAGAATGCAAAGCATCTATGGGTCATGGTGGTCTCATGGATTGTATATT
AAACTGGCATTGGTTATAGACCATGAACAATACCTTTATCGAAAAAAAAATACTTCTCTTGTGATAAGAG
ATGTATTAAGCATTATGCAGGGAATAAATCTCTTCTTACTTTCAGTGGATATTAATGTGGTTTTACTTGG
ACTTACTATCTGGACTAATGGAAATCCTATACCAGTGCAAGATATATATGCTCTTTTGCCAGCATTTTGT
ACGTGGAAGGGAACAAACCTTGATTCTCAGATACCATATGATATTGCACATCTCTTTGTGAATTATACTT
TTAGTAACTATTTTGGCATAGCCTATGTAGGAACTGTATGTGATAAGACATTTGGTTGTGGAATTGATAG
TATCGCTGAAGATGATTTCCTTACCATTGGACATATTGTGGCACATGAGATAGGTCACAATTTGGGCATG
TCACATGATGGAATACTTTGTACTTGCGGAGAAGAATCATGTTTAATGTCTGCTACAATGGATAGTTCCC
AAAAACTCAGCAACTGTAGTTATGAAGTCTTGTGGGCACACATGATCAATAAAAGCTGCATACATAGAGA
GCCCAGACCTTCTGATATTTTCCAATTGAAGGTCTGTGGGAATGGTATAGTTGAAGAAGGTGAGCAGTGT
GACTGTGGAAGCTCTGAAAACTGTAGACGTAATCGCTGTTGTATGCCTAGCTGTACTCTGAGGTCTAAAG
CTAAATGTGATACTGGACTATGTTGTAACCACAAATGCCAAATCCAGCCATCTGGCACTCTGTGTAGAGC
TCGGGAGAATGAATGTGACCTTCCAGAATGGTGCAATGGAACCTCACATGAGTGCCCTGAAGACTTGTTT
GTACAGGATGGGACCTCTTGCCCTGGTGATGGCTACTGCTATGAAAAAAGATGTAACAGCCACGATGTCC
ACTGTCAGAGAGTTTTTGGCCAGCTTGCCATGAAAGCATCGGATAGCTGTTACAAGGAACTCAATACTCG
TGGTGATCGTTTTGGTAACTGTGGCTTCATAAACAACGAGTATGTGAGATGTGAGATCTCAGACATCCTC
TGTGGGAGGATTCAATGTGACAAAGTGGGAACACTTCCTATCCTGCAGAATCATTATACTATTCACTGGA
CTCATTTTAATAGTGTTTCCTGCTGGAGTACTGACTACCATTTGGGGATGAAAATTACTGACCTCGGTGA
CATAAAAGATGGCACAAACTGTGGTCCACAGCATGTGTGCATTGACAGAAAGTGTGTTAATAAGCCAAGT
TGGGTAAATGATTGTACACCAGAGACCTGCAACATGAAAGGAGTCTGCAATAATAAGCAGCACTGCCATT
GTGATGTTGGCTGGAGCCCACCAAACTGCCAGGAAACTGGCACCGGAGGAAGTATTGACAGTGGTTCTCC
TGGAAACGAAGTCTATGAAGATGAAGTTGTGAGCAAGAAGGATGCCCCAGAAAAACCTAACGTCATAATT
TGGTTATTACCTATTATTTGTGTGGCTGTAGTTTTATCTGTATTGTTTTGCTTGTCTGGGGCCACTAAAA
AATCACGTGAAGCAGCAGCAAGTCAACCAGCAGGAGAGACAGTTAAACCACCATATGAAGGGGCTGAACC
CTCATATGAGACAGTTAAACCACCGGATGAATGGGCTAACCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216658 representing NM_010086
Red=Cloning site Green=Tags(s)

MVAMSEALVHARITLLQAWLRMLLFSSVWPPTWCAEYKGPPETVKPLRVIVSSKDMSLAGWMSYSLYFGG
QRHIISMKSKNFLESRQLPVFTYNDQGVLFEDRPFVQNDCYYLGFVDGDLESMAALTTCFGGFQGILQIN
DTAYEIKPKSPSSTFEHLLYKLDSEKTQLRPMRCGLTDEEIEGQVRLQENGKSTRMQSIYGSWWSHGLYI
KLALVIDHEQYLYRKKNTSLVIRDVLSIMQGINLFLLSVDINVVLLGLTIWTNGNPIPVQDIYALLPAFC
TWKGTNLDSQIPYDIAHLFVNYTFSNYFGIAYVGTVCDKTFGCGIDSIAEDDFLTIGHIVAHEIGHNLGM
SHDGILCTCGEESCLMSATMDSSQKLSNCSYEVLWAHMINKSCIHREPRPSDIFQLKVCGNGIVEEGEQC
DCGSSENCRRNRCCMPSCTLRSKAKCDTGLCCNHKCQIQPSGTLCRARENECDLPEWCNGTSHECPEDLF
VQDGTSCPGDGYCYEKRCNSHDVHCQRVFGQLAMKASDSCYKELNTRGDRFGNCGFINNEYVRCEISDIL
CGRIQCDKVGTLPILQNHYTIHWTHFNSVSCWSTDYHLGMKITDLGDIKDGTNCGPQHVCIDRKCVNKPS
WVNDCTPETCNMKGVCNNKQHCHCDVGWSPPNCQETGTGGSIDSGSPGNEVYEDEVVSKKDAPEKPNVII
WLLPIICVAVVLSVLFCLSGATKKSREAAASQPAGETVKPPYEGAEPSYETVKPPDEWANP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010086
ORF Size 2283 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010086.1
RefSeq Size 2870 bp
RefSeq ORF 2286 bp
Locus ID 13526
UniProt ID Q9R160
Cytogenetics 8 A4
Summary This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is expressed in a regulated fashion during spermatogenesis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional metalloprotease enzyme. Male mice lacking the encoded protein exhibit reduced fertility due to the higher incidence of polyspermic embryos. This gene is located adjacent to other ADAM genes on chromosome 8. provided by RefSeq, May 2016
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Documents
Resources
"NM_010086" in other vectors (4)
SKU Description Size Price
MC221364 Adam24 (untagged) - Mouse a disintegrin and metallopeptidase domain 24 (testase 1) (Adam24), (10ug) 10 ug
$767.00
MR216658 Adam24 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 24 (testase 1) (Adam24) 10 ug
$589.00 $697.00
MR216658L3 Lenti ORF clone of Adam24 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 24 (testase 1) (Adam24) 10 ug
$997.00
MR216658L4 Lenti ORF clone of Adam24 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 24 (testase 1) (Adam24) 10 ug
$997.00

file-alt Citations

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