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Jsrp1 (NM_028001) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216641
Jsrp1 (tGFP-tagged) - Mouse junctional sarcoplasmic reticulum protein 1 (Jsrp1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jsrp1
Synonyms 2300003C06Rik; 2310032K21Rik; JP-45; JP45
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216641 representing NM_028001
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTACCAGAGGCCTGGAGGACCTGGATGGGGGCCTGGGCAGCTGCCTCCCCAGTGATGACCTCCCAT
TTCTGGAGGAACCAGCCTCAGGCCGGCGGCCAGAGAGCAAGGCTCGAGGGACATCCAGGCGGGCCGACTC
CAGTGACTGGACCCATGTCCTTCAGGACCCAGTTGCTGCTGGCGCTGGGGATGCCGGGCTCAAGAAGATG
GAAAAAGAGCTAGCGGGCAAGGAGAGCACAGCTGGCAAAGCGGGAACAAGCCCCCGGATCGTTCCTGCAC
GTAGGAAGCCGCAGGCTCCGCCTCCCCTGCAGCCGCCCCCGCCTCCCCTGCAGCCGCCCCCAAGGACGCC
AAGCGATGACCTGCCCTGGGGAGATCTGACGCTCAACAAGTGCCTGGTACTGGCCTCGCTGGTGGCCCTG
CTGGGCTCGGCTCTTCAGCTGTGTCGGGACGCAGTGGCTGGGGAGGTCGTGGCTGCCCCTCACCCATGGG
TCCCGCCCAGCTCTCCACCAAAGAAGGAAGCGTCGCCAGCGCCTAAGCCTCCGGTTTTGGTGTCTCCATC
CGGGTCTCCTCAGCCGAAGCCTGGGCCACCCCAGGCACGGATGCAGGATGAGCCTGAGCTTCCCGGGAGC
CCTGAAGCTACAGAAACCCGGGTAGAACGTGGAGGGTCCATCTCGGAAGCCTCTGGGGAGGAGAGCGTAC
CCCTTGGGGACCGAGGGTCCCAGGAGAAGCCACGGAAAGAGAAGCCAAGTAAAGGGGAGAAACTGAAGAA
GGAGAAGCCGAGAAGGGAGAAGCCGAGGAGAGAGGATAAGTCACAGGTCACTGGGGAGCCCCGTCAATCA
CTGCCACGGCGCTGGGAGGCGCGGGAAGGAGGCCGTCGGCCATGGGGACGGGACTCCAGGGACCTCCTCG
AGCATGGGAAGCTGCAGGCCTGGGCTCCCCGGCGAAGGCATGACCGTGATGACCGGCCCAGACAGAAGCG
AGGGAAGGGGCGTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216641 representing NM_028001
Red=Cloning site Green=Tags(s)

MTTRGLEDLDGGLGSCLPSDDLPFLEEPASGRRPESKARGTSRRADSSDWTHVLQDPVAAGAGDAGLKKM
EKELAGKESTAGKAGTSPRIVPARRKPQAPPPLQPPPPPLQPPPRTPSDDLPWGDLTLNKCLVLASLVAL
LGSALQLCRDAVAGEVVAAPHPWVPPSSPPKKEASPAPKPPVLVSPSGSPQPKPGPPQARMQDEPELPGS
PEATETRVERGGSISEASGEESVPLGDRGSQEKPRKEKPSKGEKLKKEKPRREKPRREDKSQVTGEPRQS
LPRRWEAREGGRRPWGRDSRDLLEHGKLQAWAPRRRHDRDDRPRQKRGKGRD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028001
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028001.3, NP_082277.2
RefSeq Size 1325 bp
RefSeq ORF 999 bp
Locus ID 71912
Cytogenetics 10 C1
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Documents
Resources
"NM_028001" in other vectors (4)
SKU Description Size Price
MC211250 Jsrp1 (untagged) - Mouse junctional sarcoplasmic reticulum protein 1 (Jsrp1), (10ug) 10 ug
$330.00
MR216641 Jsrp1 (Myc-DDK-tagged) - Mouse junctional sarcoplasmic reticulum protein 1 (Jsrp1) 10 ug
$289.00 $300.00
MR216641L3 Lenti ORF clone of Jsrp1 (Myc-DDK-tagged) - Mouse junctional sarcoplasmic reticulum protein 1 (Jsrp1) 10 ug
$600.00
MR216641L4 Lenti ORF clone of Jsrp1 (mGFP-tagged) - Mouse junctional sarcoplasmic reticulum protein 1 (Jsrp1) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.