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Rai14 (NM_001166408) Mouse Tagged ORF Clone

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SKU
MG216638
Rai14 (tGFP-tagged) - Mouse retinoic acid induced 14 (Rai14) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,097.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Rai14 or NM_001166408"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rai14
Synonyms 1700008J19Rik; 1700020L11Rik; Ankycorbin; mKIAA1334; Norpeg
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216638 representing NM_001166408
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAGCCTGAAAGCAAAGTTTCGGAAGAGCGATACAAACGAGTGGAACAAGAATGATGACCGTCTGC
TGCAGGCTGTGGAGAACGGAGATGCTGAGAAGGTGGCCTCACTGCTGGGCAAGAAAGGGGCCAGCGCCAC
GAAGCATGACAGCGAGGGCAAGACCGCTTTCCACCTTGCCGCTGCGAAGGGACACGTGGAATGCCTCAAG
GTCATGGTGACACACGGTGTGGATGTGACTGCCCAAGATTCTTCCGGTCACAGTGCTTTGCATGTCGCAG
CCAAGAATGGCCACCCAGAGTGCATCAGGAAGCTTCTTCAGTACAAGAGCCCAGCGGAGAACATTGACAA
CTCGGGGAAAACAGCTTTGCATTATGCAGCGGCACAGGGATGCCTTCAGGCTGTACAGCTCCTCTGCGAA
CACAAGAGCCCCATAAACCTTAAAGACTTGGATGGGAACATCCCCCTGCTTGTTGCAGTCCAGAACGGCC
ACAGTGAGGCCTGCCACTTTCTCCTGGATCACGGCGCAGATGTCAATTCCAGAGACAAAAATGGAAGAAC
TGCTCTCATGCTGGCTTGTGAAACCGGCAGCTCGAACACTGTGGACGCCTTAATTAAAAAGGGAGCGGAC
CTGAGCCTTGTCGATTCTCTCGGACACAATGCCTTACATTATTCCAAACTCTCGGAAAATGCAGGAATTC
AAAACCTTCTATTATCAAAAATCTCTCAGGATGCTGATTTAAAGACTCCAACAAAACCAAAGCAGCATGA
CCAAGTCTCTAAAATAAGCTCAGAACGAAGTGGAACTCCAAAAAAGCGCAAAGCTCCACCACCTCCTATC
AGTCCTACCCAGTTGAGTGATGTGTCCTCCCCACGATCAATAACATCTACACCACTTTCAGGAAAGGAGT
CAGTATTTTTTGCTGAAGCGCCCTTTAAGGCTGAGATCAGCTCCATACAAGAAAACAAAGACAGGCTGAG
CGACAGTACTGCAGGTGCTGACAGCTTGTTGGATATAAGTTCTGAAGCTGACCAACAAGATCTTCTTGTC
CTGTTGCAAGCAAAAGTCGCTTCCCTTACCTTACACAATAAGGAGTTACAAGATAAATTGCAGGCCAAAT
CGCCCAAGGACAAGGAAGCGGAAGCCGACCTAAGCTTTCAGTCTTTCCATTCGACCCAGACTGACCTGGC
CCCATCCCCGGGCAAAGCTAGTGACATCCCTTCCTCCGATGCCAAATCATCCCCACCCGTTGAGCATCCG
GCGGGGACGTCCACCACTGACAATGATGTCATAATCCGGCAGCTCCAAGATTCCCTGCATGATCTGCAGA
AGAGATTGGAGAGCTCCGAAGCAGAGAAGAAACAGCTCCAGGACGAACTCCAGTCACAAAGGACAGACAC
GCTTTGCTTGAACAACACAGAGATCTCCGAGAATGGCTCTGACCTCAGTCAGAAGCTCAAGGAAACCCAG
AGCAAGTACGAAGAGGCCATGAAAGAAGTCCTGAGCGTGCAGAAACAGATGAAGCTGGGTCTCCTCTCCC
AGGAGAGCGCCGATGGCTACTCGCATCTCCGGGAGGCGCCAGCTGACGAAGACATCGACACACTAAAGCA
AGACCTGCAGAAAGCGGTGGAAGAAAGTGCAAGGAACAAGGAGCGGGTGCGGGAGTTAGAGACTAAGCTT
GCGGAGAAGGAGCAGGCCGAAGCGACTAAGCCACCTGCCGAGGCGTGTGAGGAGCTGAGAAGTTCCTACT
GCTCTGTTATCGAGAATATGAATAAGGAGAAGGCATTTTTGTTTGAGAAATATCAACAGGCCCAAGAAGA
GATCATGAAACTGAAGGACACGCTAAAAAGTCAGATGCCACAGGAAGCCCCCGACGACTCTGGGGATATG
AAAGAGGCCATGAACAGGATGATTGATGAACTCAATAAACAGGTGAGCGAGCTGTCACAGCTGTACCGAG
AAGCCCAGGCAGAGCTGGAGGATTACAGGAAGAGGAAATCTCTGGAAGATGCAGCCGAGTACATTCACAA
AGCTGAACACGAGAGGCTGATGCACGTGTCAAACCTGTCCAGGGCCAAGTCGGAAGAAGCGCTGTCGGAG
ATGAAGTCTCAGTATTCCAAAGTGTTGAACGAGTTGACTCAGCTCAAACAGCTGGTGGATGCACACAAAG
AGAACTCCGTGTCCATCACAGAGCATTTGCAAGTGATAACCACGCTGCGGACAACGGCCAAAGAGATGGA
AGAAAAGATAAGCGCCCTCACAGGGCACCTTGCAAACAAGGAAGCGGAAGTGGCAAAGCTGGAGAAGCAG
CTCGCCGAAGAGAAGGCTGCCGTGAGCGACGCGATGGTCCCCAAGTCTTCCTACGAGAAGCTCCAGGCCT
CCTTGGAGAGCGAAGTCAATGCCTTAGCAACAAAGTTAAAGGAGTCAGTAAGAGAGAGAGAGAAAGCCCA
TTCCGAGGTGGCCCAGGTTCGAAGTGAGGTCTCACAGGCGAGAAGGGAAAAGGACAACATCCAGACTCTC
TTGAAAGCCAAAGAGCAGGAAGTAACTGCGCTTGTGCAGAAGTTCCAGCGAGCTCAGGAAGAACTGGCTG
GGATGCGAAGATGCTCTGAGACCTCGTCGAAGCTGGAGGAGGATAAAGATGAAAAGATAAATGAAATGAC
CAGGGAAGTCCTGAAGCTGAAGGAGGCCCTGAACAGCCTCTCGCAGCTCTCCTACTCCACAAGTTCATCC
AAGAGGCAGAGCCAGCAACTGGACCTGCTACAGCAGCAGGTCAAGCAGCTGCAGAACCAGCTGGCTGAAT
GTAAGAAACACCACCAGGAGGTCATCTCAGTTTACAGAATGCATCTTCTATATGCCGTTCAGGGCCAAAT
GGATGAAGATGTCCAGAAGGTCTTGAAGCAGATCCTCACCATGTGTAAAAACCAGTCTCAGAAGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216638 representing NM_001166408
Red=Cloning site Green=Tags(s)

MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLK
VMVTHGVDVTAQDSSGHSALHVAAKNGHPECIRKLLQYKSPAENIDNSGKTALHYAAAQGCLQAVQLLCE
HKSPINLKDLDGNIPLLVAVQNGHSEACHFLLDHGADVNSRDKNGRTALMLACETGSSNTVDALIKKGAD
LSLVDSLGHNALHYSKLSENAGIQNLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPI
SPTQLSDVSSPRSITSTPLSGKESVFFAEAPFKAEISSIQENKDRLSDSTAGADSLLDISSEADQQDLLV
LLQAKVASLTLHNKELQDKLQAKSPKDKEAEADLSFQSFHSTQTDLAPSPGKASDIPSSDAKSSPPVEHP
AGTSTTDNDVIIRQLQDSLHDLQKRLESSEAEKKQLQDELQSQRTDTLCLNNTEISENGSDLSQKLKETQ
SKYEEAMKEVLSVQKQMKLGLLSQESADGYSHLREAPADEDIDTLKQDLQKAVEESARNKERVRELETKL
AEKEQAEATKPPAEACEELRSSYCSVIENMNKEKAFLFEKYQQAQEEIMKLKDTLKSQMPQEAPDDSGDM
KEAMNRMIDELNKQVSELSQLYREAQAELEDYRKRKSLEDAAEYIHKAEHERLMHVSNLSRAKSEEALSE
MKSQYSKVLNELTQLKQLVDAHKENSVSITEHLQVITTLRTTAKEMEEKISALTGHLANKEAEVAKLEKQ
LAEEKAAVSDAMVPKSSYEKLQASLESEVNALATKLKESVREREKAHSEVAQVRSEVSQARREKDNIQTL
LKAKEQEVTALVQKFQRAQEELAGMRRCSETSSKLEEDKDEKINEMTREVLKLKEALNSLSQLSYSTSSS
KRQSQQLDLLQQQVKQLQNQLAECKKHHQEVISVYRMHLLYAVQGQMDEDVQKVLKQILTMCKNQSQKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166408
ORF Size 2937 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166408.1, NP_001159880.1
RefSeq Size 4843 bp
RefSeq ORF 2940 bp
Locus ID 75646
UniProt ID Q9EP71
Cytogenetics 15 A1
Summary Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001166408" in other vectors (4)
SKU Description Size Price
MC222953 Rai14 (untagged) - Mouse retinoic acid induced 14 (Rai14), transcript variant 2, (10ug) 10 ug
$987.00
MR216638 Rai14 (Myc-DDK-tagged) - Mouse retinoic acid induced 14 (Rai14), transcript variant 2 10 ug
$589.00 $897.00
MR216638L3 Lenti ORF clone of Rai14 (Myc-DDK-tagged) - Mouse retinoic acid induced 14 (Rai14), transcript variant 2 10 ug
$1,197.00
MR216638L4 Lenti ORF clone of Rai14 (mGFP-tagged) - Mouse retinoic acid induced 14 (Rai14), transcript variant 2 10 ug
$1,197.00

file-alt Citations

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