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Qrich1 (NM_175143) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216623
Qrich1 (tGFP-tagged) - Mouse glutamine-rich 1 (Qrich1) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $911.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Qrich1 or NM_175143"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Qrich1
Synonyms 2610028H07Rik; b2b2404Clo
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216623 representing NM_175143
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAGAGGTAGTGCAGACCCTTGCAAACTCTCTCT
TTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGTGGCTGTGCAGGCTGTAGCAGGCACATACCA
GAATACGGCTCAGACTGTACATATATGGGACCCACAGCAGCAGCCACAACAGCAAACTGCACAAGAGCAG
ACACCACCACCACAACAGCAGCAGCAGCAGCTGCAGGTCACTTGTTCAGCACAGACTGTTCAGGTTGCTG
AAGTTGAGCCACAGTCACAACCACAGCCTTCACCAGAGCTTTTGCTTCCAAATTCTTTGAAGCCAGAAGA
AGGGCTTGAAGTATGGAAAAACTGGGCTCAGACCAAGAATGCTGAACTAGAAAAGGATGCTCAAAACAGA
TTGGCCCCCATTGGGAGGCGACAGTTGCTACGATTTCAGGAAGATCTGATCTCCTCTGCTGTGGCTGAAT
TGAATTATGGCCTTTGTTTAATGACACGAGAAGCTCGGAATGGAGAAGGTGAACCCTATGACCCAGATGT
ACTCTACTACATTTTCCTGTGTATACAGAAGTATCTTTTTGAAAATGGAAGAGTAGATGACATATTTTCT
GATCTTTACTATGTTCGTTTCACCGAATGGCTACATGAAGTTCTGAAGGATGTACAGCCTCGGGTCACTC
CACTTGGCTATGTGTTGCCCAGTCACGTGACTGAAGAGATGCTGTGGGAATGTAAGCAGCTTGGGGCACA
TTCCCCTTCCACACTGCTGACCACCCTCATGTTCTTTAACACCAAGTACTTCCTGTTGAAGACTGTAGAT
CAGCACATGAAGCTGGCTTTCTCTAAGGTCCTCCGACAGACAAAGAAGAGCCCCTCGAATCCTAAGGATA
AAAGCACGAGCATCCGCTACCTGAAGGCCCTCGGAATACACCAGACTGGCCAGAAAGTTACAGATGACAT
GTATGCAGAGCAAACAGAGAATCCAGAGAATCCACTAAGGTGTCCCATCAAGCTCTACGATTTCTACCTC
TTTAAATGCCCTCAGAGTGTGAAAGGCCGAAATGACACCTTTTACCTGACACCTGAGCCAGTTGTAGCTC
CCAACAGCCCAATCTGGTACTCAGTCCAGCCTATCAGCAGAGAACAGATGGGACAGATGCTGACCCGGAT
CCTGGTTATACGAGAAATCCAAGAAGCCATTGCCGTGGCCAATGCAACCACCATGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216623 representing NM_175143
Red=Cloning site Green=Tags(s)

MVGTTSVVKNSHEEVVQTLANSLFPAQFMNGNIHIPVAVQAVAGTYQNTAQTVHIWDPQQQPQQQTAQEQ
TPPPQQQQQQLQVTCSAQTVQVAEVEPQSQPQPSPELLLPNSLKPEEGLEVWKNWAQTKNAELEKDAQNR
LAPIGRRQLLRFQEDLISSAVAELNYGLCLMTREARNGEGEPYDPDVLYYIFLCIQKYLFENGRVDDIFS
DLYYVRFTEWLHEVLKDVQPRVTPLGYVLPSHVTEEMLWECKQLGAHSPSTLLTTLMFFNTKYFLLKTVD
QHMKLAFSKVLRQTKKSPSNPKDKSTSIRYLKALGIHQTGQKVTDDMYAEQTENPENPLRCPIKLYDFYL
FKCPQSVKGRNDTFYLTPEPVVAPNSPIWYSVQPISREQMGQMLTRILVIREIQEAIAVANATTMH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175143
ORF Size 1248 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175143.5, NP_780352.2
RefSeq Size 3238 bp
RefSeq ORF 2334 bp
Locus ID 69232
UniProt ID Q3UA37
Cytogenetics 9 F2
Summary Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR), an ER-stress response pathway that either promotes recovery of ER homeostasis and cell survival, or triggers the terminal UPR which elicits programmed cell death when ER stress is prolonged and unresolved (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (By similarity). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (By similarity). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175143" in other vectors (4)
SKU Description Size Price
MC221500 Qrich1 (untagged) - Mouse glutamine-rich 1 (Qrich1), transcript variant 2, (10ug) 10 ug
$784.00
MR216623 Qrich1 (Myc-DDK-tagged) - Mouse glutamine-rich 1 (Qrich1), transcript variant 2 10 ug
$589.00 $711.00
MR216623L3 Lenti ORF clone of Qrich1 (Myc-DDK-tagged) - Mouse glutamine-rich 1 (Qrich1), transcript variant 2 10 ug
$1,011.00
MR216623L4 Lenti ORF clone of Qrich1 (mGFP-tagged) - Mouse glutamine-rich 1 (Qrich1), transcript variant 2 10 ug
$1,011.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.