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Vps51 (NM_001081041) Mouse Tagged ORF Clone

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SKU
MG216602
Vps51 (tGFP-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $916.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vps51
Synonyms 1110014N23Rik; 3110057M17Rik; AI837850; Ffr
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216602 representing NM_001081041
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCGGCAGCTGTGGGGCCTGGCCTAGGGTCCGGGCCTGGAGACTCCCCAGAAGGTCCCGAGG
CGGACGCTCCGGAGCGTCGGCGGAAGGCTCACGGGATGCTGAAGCTTTACTACGGCCTCTCGGAGGGGGA
GGTGGCGGGGCATCCCGCAGGGCCTGACCCTCTGGACCCAACCGATCTCAATGGGGCGCACTTCGACCCG
GAAGTTTATCTTGACAAGCTGCGTAGAGAATGTCCTCTGGCCCAGCTGATGGACAGTGAGACGGACATGG
TACGGCAGATCCGGGCTCTAGACAGCGACATGCAGACCCTGGTCTATGAGAACTACAACAAGTTCATCTC
AGCTACAGACACCATTCGAAAGATGAAGAATGACTTCCGGAAGATGGAAGATGAGATGGACCGGCTGGCC
ACCAACATGGCAGTGATCACCAACTTCAGCGCACGGATCAGCGCCACGCTACAGGACCGACACGAGCGCA
TCACCAAGCTAGCAGGGGTACATGCACTACTGCGGAAGCTGCAGTTCCTTTTTGAGCTGCCTTCGCGCCT
CACCAAGTGTGTGGAGCTGGGTGCCTATGGGCAGGCAGTGCGCTATCAGGGTCGTGCCAGGGCCGTGCTG
CAGCAGTACCAGCACCTGCCCTCCTTCCGTGCCATACAGGATGACTGCCAGGTCATCACAGCTCGCTTGG
CCCAGCAGCTGCGGCAGCGCTTCAGGGAAGGCTGCTCAGGTGCCCCGGAGCAGGCCGAGTGTGTGGAGCT
GCTGCTGGCCCTGGGCGAGCCAGCTGAGGAGCTGTGCGAGGAGTTCCTGGCGCATGCGCGGGGACGGCTG
GAGGAAGAACTGAGCAGCCTGGAGGCCGAACTGGGGCCCTCCCCTCCAGCACCTGATGTGTTAGAGTTCA
CCGACCGTGGTGGCAATGGTTTTGTAGGTGGCCTCTGCCAGGTGGCAGCAGCCTACCAAGAGCTGTTTGC
TGCTCAGGGCCCTGCTGGTGCGGAGAAGCTGGCAGCCTTTGCGCAGGAGCTGGGCGGCCGCTACTTTGCA
CTGGTTGAGCGGCGGCTGGCACAGGAGCAGGGTGGTAGTGACAACTCACTGCTGGTGCGGGCACTAGATC
GCTTCCACCGGCGCCTGCGAGCACCTGGGGCCCTGCTGGCCGCTGCTGGGCTTTCAGAGTCAGCCACAGA
GATTGTGGAGCGAGTGGCCCGGGAGCGCCTGAGCCACCATCTGCAGGGTCTGAAGGCTGCCTTCCTGAGC
TCCCTGACGGATGTTCGCCAGGCCCTGGCAGCACCTCGCCTAGCGGGGAAGGAGGGCCCCAGCCTGGCTG
AGTTGCTGGCCAATGTGGCCAGCTCCATCCTAAGCCACATCAAGACGTCACTGGCTTCTGTGCATCTCTT
TACCGCCAAGGAGGTGTCCTTCTCCAATAAGCCCTACTTCAGGGGCGAGTTCTGCAGCCAGGGAGTTCGT
GAGGGCCTCATCGTGGGTTTCATCCGCTCCATGTGCCAGACAGCCCAGAGCTTCTGTGACAGCCCTGGAG
AAAAGGGAGGTGCCACGCCGCCTGCCCTGCTCCTGCTGCTCTCTCGCCTCTGCCTGGATTATGAGACAGC
CACCATCTCCTACATCCTCACACTCACTGATGAACAGTTTCTGGTGCAGGACCAGTCTCCCGTTACACCC
GTGAGCACACTGTGTGCAGAGGCCAGAGAGACAGCACGGCGGCTGCTGACTCACTATGTGAAGGTGCAAG
GCCTGGTCATATCCCAGATGCTACGTAAGAGTGTGGAGACCCGGGACTGGCTCAGTACTCTGGAGCCTAG
GAACGTGCGTGCTGTCATGAAGCGTGTGGTGGAGGACACTACAGCTATTGATGTCCAGGTGGGGCTCCTG
TATGAAGAGGGTGTCCGGAAGGCCCAGAGCAGTGACTCAAGCAAAAGGACTTTCTCTGTGTACAGCAGCT
CCCGGCAGCAGGGCCGCTATGCACCCAGCTACACACCCAGTGCTCCCATGGACACAAACCTCCTGAGCAA
TATTCAGAAGCTGTTCTCTGAACGGATCGATGTGTTCAGCCCCGTGGAATTCAACAAGGTGTCGGTGCTG
ACTGGCATCATTAAGATCAGCTTGAAGACACTACTGGAGTGTGTGCGCCTACGCACCTTTGGGCGCTTTG
GGCTGCAGCAGGTGCAGGTGGACTGCCACTTCCTGCAGCTTTACTTGTGGCGCTTTGTGGCTGATGAGGA
GCTAGTGCACCTGCTGCTGGACGAAGTAGTAGCCTCAGCTGCTCTGCGCTGTCCCGACCCAGTGCCCATG
GAACCTAGTGTTGTCGAGGTCATCTGCGAGCGCGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216602 representing NM_001081041
Red=Cloning site Green=Tags(s)

MAAAAAVGPGLGSGPGDSPEGPEADAPERRRKAHGMLKLYYGLSEGEVAGHPAGPDPLDPTDLNGAHFDP
EVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLA
TNMAVITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVL
QQYQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPEQAECVELLLALGEPAEELCEEFLAHARGRL
EEELSSLEAELGPSPPAPDVLEFTDRGGNGFVGGLCQVAAAYQELFAAQGPAGAEKLAAFAQELGGRYFA
LVERRLAQEQGGSDNSLLVRALDRFHRRLRAPGALLAAAGLSESATEIVERVARERLSHHLQGLKAAFLS
SLTDVRQALAAPRLAGKEGPSLAELLANVASSILSHIKTSLASVHLFTAKEVSFSNKPYFRGEFCSQGVR
EGLIVGFIRSMCQTAQSFCDSPGEKGGATPPALLLLLSRLCLDYETATISYILTLTDEQFLVQDQSPVTP
VSTLCAEARETARRLLTHYVKVQGLVISQMLRKSVETRDWLSTLEPRNVRAVMKRVVEDTTAIDVQVGLL
YEEGVRKAQSSDSSKRTFSVYSSSRQQGRYAPSYTPSAPMDTNLLSNIQKLFSERIDVFSPVEFNKVSVL
TGIIKISLKTLLECVRLRTFGRFGLQQVQVDCHFLQLYLWRFVADEELVHLLLDEVVASAALRCPDPVPM
EPSVVEVICERG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081041
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081041.1, NP_001074510.1
RefSeq Size 2631 bp
RefSeq ORF 2349 bp
Locus ID 68505
UniProt ID Q3UVL4
Cytogenetics 19 A
Summary Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081041" in other vectors (4)
SKU Description Size Price
MC221574 Vps51 (untagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug) 10 ug
$789.00
MR216602 Vps51 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik) 10 ug
$589.00 $716.00
MR216602L3 Lenti ORF clone of Vps51 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik) 10 ug
$1,016.00
MR216602L4 Lenti ORF clone of Vps51 (mGFP-tagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik) 10 ug
$1,016.00

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