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Hoxd1 (NM_010467) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216599
Hoxd1 (tGFP-tagged) - Mouse homeobox D1 (Hoxd1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hoxd1
Synonyms Hox-4.9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216599 representing NM_010467
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCTTACCTGGAGTACGTGTCGTGCGCCGCGGGCGGTGGCAGCGGAGGGGTAGGCGGCGACGTGC
TCGGCTTCGCACCCAAGTTCTGCCGCGCCGACGCGCGCCCTGTGGCCCTGCAGCCGGCCTTCCCCCTGGG
CAGCGGAGACGGCGCCTTCGTGAGCTGCCTGCCCCTGGCCACCGCTCGGCCCACGCCGTCGCCTCCCGCA
GGCCCCGCTCAGTCCCCGGTCCCACAGCCCGCCGCGCCCCGGTACGCGCCCTGCACCCTGGAAGGCGCCT
ACGAGCGGGGCGCCGCACCTGCCTCTGCCGCCGAGTATGGCTTCTTGGGGTCCGGGCCGGCGTTTGACTT
CCCGGGCGCACTTGGGAGAGCGGCGGACGAGGGCGGGGCGCACGTCCACTATGCCACCTCGGCGGTCTTC
TCCGGCGGGGGATCATTCCTGCTCAGCGGCCAGGTGGACTTCGCTGCCTTTGGCGAGCCTGGCCCCTTCC
CCGCGTGTCTCAAGGAGCCCGCAGACGGCCACCCCGGGCCCTTTCAGACTGTGTCCCCGGCCCCTGGAGC
CTGCCCCAAGCCGGCCTCTCCCACCTCCAGCCTCCCGGCCGCCCACAGCACTTTCGAGTGGATGAAAGTG
AAGAGGAACGCCCCCAAGAAAAGCAAACTGTCCGAATATGGAGCCACAAGCCCTCCCAGTGCCATCCGCA
CAAATTTCAGCACCAAGCAACTGACAGAGCTAGAGAAAGAGTTTCATTTCAATAAGTACCTAACTAGAGC
CCGACGCATCGAGATAGCCAACTGTTTACAGCTGAATGACACCCAGGTCAAAATCTGGTTCCAGAACCGT
AGGATGAAGCAGAAGAAGAGGGAACGAGAGGGGCTTCTGGCCACAGCTGCCTCTGTGGCCTCGATTAAGC
TTCCCCGGTCAGAAACAAGTCCCATCAAATCTGGCCGGAATCTAGGAAGCCCTTCTCAGGCTCAAGAGCC
TTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216599 representing NM_010467
Red=Cloning site Green=Tags(s)

MSSYLEYVSCAAGGGSGGVGGDVLGFAPKFCRADARPVALQPAFPLGSGDGAFVSCLPLATARPTPSPPA
GPAQSPVPQPAAPRYAPCTLEGAYERGAAPASAAEYGFLGSGPAFDFPGALGRAADEGGAHVHYATSAVF
SGGGSFLLSGQVDFAAFGEPGPFPACLKEPADGHPGPFQTVSPAPGACPKPASPTSSLPAAHSTFEWMKV
KRNAPKKSKLSEYGATSPPSAIRTNFSTKQLTELEKEFHFNKYLTRARRIEIANCLQLNDTQVKIWFQNR
RMKQKKREREGLLATAASVASIKLPRSETSPIKSGRNLGSPSQAQEPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010467
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010467.2, NP_034597.2
RefSeq Size 1878 bp
RefSeq ORF 987 bp
Locus ID 15429
UniProt ID Q01822
Cytogenetics 2 44.13 cM
Summary Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010467" in other vectors (4)
SKU Description Size Price
MC208696 Hoxd1 (untagged) - Mouse homeobox D1 (Hoxd1), (10ug) 10 ug
$330.00
MR216599 Hoxd1 (Myc-DDK-tagged) - Mouse homeobox D1 (Hoxd1) 10 ug
$289.00 $300.00
MR216599L3 Lenti ORF clone of Hoxd1 (Myc-DDK-tagged) - Mouse homeobox D1 (Hoxd1) 10 ug
$600.00
MR216599L4 Lenti ORF clone of Hoxd1 (mGFP-tagged) - Mouse homeobox D1 (Hoxd1) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.