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Zfp574 (NM_175477) Mouse Tagged ORF Clone

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SKU
MG216571
Zfp574 (tGFP-tagged) - Mouse zinc finger protein 574 (Zfp574) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
  $1,025.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp574
Synonyms A630056B21Rik; Znf574
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216571 representing NM_175477
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGAGGAGAGTGAAGAGACGGTCCTGTACATTGAGCACCGCTATGTCTGCTCTGAGTGCAACCAGC
TCTATGGATCCTTGGAGGAGGTGCTTGTGCACCAGAACTCCCACGTGCCCCAGCAGCACTTTGAGCTGGT
GGGCGTGGCTGACCCTGGAGTCACAGTGGCCACAGAGGCTGCTTCAGGCACAGGCCTATATCAGACCCTC
ATCCAAGAGAGCCAGTACCAGTGTCTTGAGTGTGGGCAACTGCTCCTGTCCCCCAGCCAGCTCCTGGAGC
ACCAGGAGCTACACCTGAAGATGATGGCACCGCAGGAGGCAGTGCCAGCCAAGCCACCACCCAAGGTGCC
CCCCTTGGGCTCCAGCGCCATCCACTATGAGTGTGTGGACTGTAAGGCTCTGTTCGCCAGCCAAGAGATG
TGGCTTAGCCATCGCCAGACGCATCTCAGAGCCACGCCCAACAAGGCTCCTGCTCCTGTCGTCTTGGGGT
CCCCAGTTGTTTTGGGCCCTCCTGTGGGCCAGGCTCGAGTGGCTGTGGAACATTCATACCGAAAAGCAGA
AGAGGGTGGGGAGGGGGCGGCCGTTCCGTCTGTGGCCGCCACCACCGAGATGGTGACAGAAGTGGAGCTG
CTCCTCTACAAGTGCTCCGAGTGCTCCCAGCTCTTCCAGATGCCCGCTGACTTCCTGGAGCACCAGGCCA
CCCACTTCCCTGCTCCTGTCCCGGAGGCTGCGGAGCCTGCCACACAGCAGGAAACCCAGGTCCCCTCACC
CACTGAGGCAGCAGTGTCTCAGCCCGAACCCCTGCCAGCCTCTGATCACAGTTATGAGCTGCGGAATGAG
CTGCGAAATGGGGAGGCCATTGGGCGAGACCGCAGGGGACGGAAGCCAAGGAGGAACAACAGTGGAGAGT
CAGGTGGGGCGGCCACCCAGGAACTATTTTGCTCTGCCTGTGACCAGCTCTTTCTCTCACCCCACCAGCT
ACAGCAGCACCTTCGGAGTCACCGGGAGGGTGTTTTTAAGTGTCCCCTGTGCAGTCGTGTCTTCCCTAGC
CCTTCTAGTCTGGACCAGCACCTTGGTGACCATAGCAGTGAGTCTCACTTCCTGTGTGTAGACTGTGGCC
TAGCCTTTGGCACCGAAGCCCTCCTCCTGGCCCACCGGCGAGCCCATACCCCGAATCCTCTGCATTCGTG
TCCCTGTGGGAAGACCTTTGTTAACCTCACCAAGTTCCTTTATCACCGGCGTACCCACGGAGCAGGAGGT
GTCCCTTTGCCCACAACACCAGTTCCACCAGAGGAGCCTGCTATTAGCTTTCCTGAGCCAGCCCCTGCAG
AGACCGGAGAGCTAGAGGCCCCAGAGCTCCCCGTATGTGAGGAGAGTTCAGCCGAGCCTGCAGCTCCAGG
CTCTTACCGCTGCCTCCTGTGTAGCCGGGAGTTTGGTAAGGCGTTGCAGCTGACCCGGCACCAGCGTTTT
GTGCACCGGCTAGAGCGGCGCCATAAGTGCAGCATTTGTGGCAAGATGTTCAAGAAGAAGTCTCATGTGC
GGAACCATCTGCGTACACACACTGGGGAGCGGCCCTTCCCCTGCCCTGACTGCTCTAAGCCTTTCAACTC
ACCAGCCAACCTGGCCCGCCACCGGCTCACACACACGGGGGAGCGGCCCTACCGGTGTGGGGACTGCGGC
AAGGCCTTCACGCAGAGCTCCACTCTGAGGCAGCATCGCCTGGTGCATGCCCAGCACTTCCCCTACCGCT
GCCAGGAGTGCGGAGTCCGTTTTCACCGCCCTTATCGCCTGCTCATGCACCGCTACCACCACACAGGCGA
GTACCCCTACAAGTGCCGTGAGTGCCCCCGTTCCTTCTTGCTACGCCGGCTGCTAGAGGTCCACCAGCTT
GTGATCCATGCTGGGCGCCAGCCCTACCGCTGCTCATCCTGTGGGGCCGCCTTCCCCTCGTCACTGCGGC
TCCGTGAGCATCGCTGCGCGGCCGCTGCTGCCCAGGCCCCACGGCGCTTTGAGTGTGGGACCTGTGGCAA
GAAAGTGGGCTCTGCTGCTCGCCTGCAGGCACATGAAGCCGCCCATGCTGCTGCGGGTCCTGGAGAGGTC
TTAGCCAAGGAGCCTCCTGCTCCCCGGGCCTCGAGAGCCACTCGCACACCAGTCGCCCCCTCCCCGACAG
CCCTCAGTGGTACAACCTCTGCTGCCCCTGCAGCCCCTGCCCGGCGTCGGGGTCCGGAGTGCAGTGAGTG
CAAGAAGCTCTTCAGCACAGAGACGTCACTACAGGTCCATCGGCGCATCCACACAGGTGAGCGGCCATAC
CCCTGTCCAGACTGTGGCAAGGCCTTCCGCCAGAGTACCCATCTGAAAGACCACAGACGCCTACACACAG
GCGAGCGGCCCTTTGCGTGTGAAGTGTGTGGCAAGGCCTTTGCCATCTCCATGCGTCTGGCAGAACATCG
CCGCATCCACACGGGTGAGCGACCCTACTCCTGTCCTGACTGTGGCAAGAGCTACCGTTCCTTCTCCAAT
CTCTGGAAGCACCGCAAGACCCACCAGCAGCAACACCAGGCGGCCGTCCGACAGCAGCTGGCAGAGGCGG
AGGCTGCTGTGGGCCTGGCTGTGATGGAAACTGCAGTGGAGGCTCTGCCCCTTGTGGAGGCCATTGAGAT
CTACCCTCTAGCTGAAGCTGATGGGGTCCAGATCAGTGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216571 representing NM_175477
Red=Cloning site Green=Tags(s)

MTEESEETVLYIEHRYVCSECNQLYGSLEEVLVHQNSHVPQQHFELVGVADPGVTVATEAASGTGLYQTL
IQESQYQCLECGQLLLSPSQLLEHQELHLKMMAPQEAVPAKPPPKVPPLGSSAIHYECVDCKALFASQEM
WLSHRQTHLRATPNKAPAPVVLGSPVVLGPPVGQARVAVEHSYRKAEEGGEGAAVPSVAATTEMVTEVEL
LLYKCSECSQLFQMPADFLEHQATHFPAPVPEAAEPATQQETQVPSPTEAAVSQPEPLPASDHSYELRNE
LRNGEAIGRDRRGRKPRRNNSGESGGAATQELFCSACDQLFLSPHQLQQHLRSHREGVFKCPLCSRVFPS
PSSLDQHLGDHSSESHFLCVDCGLAFGTEALLLAHRRAHTPNPLHSCPCGKTFVNLTKFLYHRRTHGAGG
VPLPTTPVPPEEPAISFPEPAPAETGELEAPELPVCEESSAEPAAPGSYRCLLCSREFGKALQLTRHQRF
VHRLERRHKCSICGKMFKKKSHVRNHLRTHTGERPFPCPDCSKPFNSPANLARHRLTHTGERPYRCGDCG
KAFTQSSTLRQHRLVHAQHFPYRCQECGVRFHRPYRLLMHRYHHTGEYPYKCRECPRSFLLRRLLEVHQL
VIHAGRQPYRCSSCGAAFPSSLRLREHRCAAAAAQAPRRFECGTCGKKVGSAARLQAHEAAHAAAGPGEV
LAKEPPAPRASRATRTPVAPSPTALSGTTSAAPAAPARRRGPECSECKKLFSTETSLQVHRRIHTGERPY
PCPDCGKAFRQSTHLKDHRRLHTGERPFACEVCGKAFAISMRLAEHRRIHTGERPYSCPDCGKSYRSFSN
LWKHRKTHQQQHQAAVRQQLAEAEAAVGLAVMETAVEALPLVEAIEIYPLAEADGVQISG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175477
ORF Size 2700 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175477.5
RefSeq Size 4195 bp
RefSeq ORF 2703 bp
Locus ID 232976
UniProt ID Q8BY46
Cytogenetics 7 A3
Summary May be involved in transcriptional regulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175477" in other vectors (3)
SKU Description Size Price
MC222492 Zfp574 (untagged) - Mouse zinc finger protein 574 (Zfp574), transcript variant 1, (10ug) 10 ug
$907.00
MG211099 Zfp574 (tGFP-tagged) - Mouse zinc finger protein 574 (Zfp574) 10 ug
$1,025.00
MR216571 Zfp574 (Myc-DDK-tagged) - Mouse zinc finger protein 574 (Zfp574), transcript variant 1 10 ug
$589.00 $825.00

file-alt Citations

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