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Puf60 (NM_001164600) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216560
Puf60 (tGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Puf60
Synonyms 2410104I19Rik; 2810454F19Rik; SIAHBP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216560 representing NM_001164600
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAATGGACAAAGCACAGGCACCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCC
TCCAGAAGGCCAAGAAATATGCAATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAACAGACCATCGCGCA
CCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCTCAACGGCAGCGGGCACTGGCTATCATGTGCCGG
GTCTATGTGGGCTCCATCTACTATGAGCTGGGAGAAGACACTATTCGCCAGGCCTTTGCTCCCTTTGGCC
CCATCAAGAGCATTGATATGTCCTGGGACTCCGTTACCATGAAGCATAAGGGCTTTGCCTTCGTGGAGTA
CGAGGTCCCAGAAGCTGCGCAGCTCGCTTTGGAGCAGATGAACTCTGTGATGCTTGGGGGCAGGAACATC
AAGGTGGGAAGACCCAGCAACATTGGACAAGCCCAACCCATCATAGATCAGCTGGCTGAGGAAGCTAGGG
CTTTCAACCGAATATATGTGGCCTCTGTACATCAAGACCTGTCTGATGATGACATCAAGAGTGTATTTGA
AGCCTTTGGCAAGATCAAGTCTTGTACGCTGGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTATGGT
TTTATCGAATATGAGAAGGCCCAGTCGTCCCAGGATGCTGTGTCCTCCATGAACCTCTTTGATCTGGGTG
GCCAGTACTTGAGGGTGGGCAAGGCCGTCACACCCCCCATGCCCCTGCTAACACCTGCCACGCCTGGAGG
TCTCCCGCCTGCTGCTGCTGTGGCCGCAGCTGCAGCCACGGCCAAGATTACAGCTCAGGAAGCAGTGGCT
GGAGCAGCAGTGCTGGGTACTTTAGCCACACCAGGACTAGTGTCCCCAGCACTGACACTGGCCCAGCCCT
TAGGGGCCCTACCCCAGGCTGTCATGGCTGCCCAGGCCCCTGGAGTCATCACAGGTGTGACACCAGCCCG
CCCTCCTATTCCGGTCACCATCCCCTCTGTGGGAGTAGTGAACCCCATTCTCGCCAGCCCGCCAACGTTG
GGTCTGTTGGAGCCCAAGAAGGAGAAGGAAGAGGAGGAGCTGTTTCCTGAGTCAGAACGGCCAGAGATGT
TGAGTGAGCAGGAGCACATGAGCATCTCGGGCAGCAGTGCTCGCCACATGGTCATGCAAAAGCTACTCAG
AAAACAGGAGTCTACAGTGATGGTTCTCCGAAACATGGTGGACCCCAAGGACATCGATGACGACCTGGAG
GGAGAGGTGACAGAGGAGTGTGGCAAATTTGGTGCTGTGAACCGGGTCATTATCTATCAAGAAAAACAGG
GCGAAGAGGAAGATGCAGAAATCATCGTCAAGATTTTTGTGGAATTCTCCATGGCCTCAGAGACTCACAA
GGCCATCCAGGCCCTCAATGGGCGCTGGTTTGGTGGTCGCAAGGTGGTGGCTGAAGTGTATGACCAGGAG
CGTTTTGATAACAGCGACCTCTCCGCG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216560 representing NM_001164600
Red=Cloning site Green=Tags(s)

MENGQSTGTKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCR
VYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNI
KVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYG
FIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVA
GAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTL
GLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLE
GEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQE
RFDNSDLSA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164600
ORF Size 1497 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164600.1, NP_001158072.1
RefSeq Size 1848 bp
RefSeq ORF 1500 bp
Locus ID 67959
UniProt ID Q3UEB3
Cytogenetics 15 D3
Summary DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001164600" in other vectors (4)
SKU Description Size Price
MC217008 Puf60 (untagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 3, (10ug) 10 ug
$503.00
MR216560 Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 3 10 ug
$289.00 $457.00
MR216560L3 Lenti ORF clone of Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 3 10 ug
$757.00
MR216560L4 Lenti ORF clone of Puf60 (mGFP-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 3 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.