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4931440F15Rik (NM_176829) Mouse Tagged ORF Clone

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SKU
MG216550
4931440F15Rik (tGFP-tagged) - Mouse RIKEN cDNA 4931440F15 gene (4931440F15Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $810.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 4931440F15Rik
Synonyms Fem1ab
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216550 representing NM_176829
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTGCACACAGCGGTGTACAATGCGGCGCACGACGGCAAGCTGCCTCTGCTGCAGAAACTGTTGG
CCAGCCGTGGGCGTGAGGAGCTGGAGGAGCTGCTGGGTGAGGTGGCCGGCGGTGGAACGCCACTGCTAAT
CGCAGCGCGCCGTGGACACCTAGACGTGGTGGAGTATCTGGTGGACCACTGCGGCGCCAGCGTGGAGGCG
AGTGGCTCTGTGCACTTCGATGGCGAGACCATCGAGGGCGCGCCGCCGCTCTGGGCAGCGTCGGCCGCGG
GTCACCTGGCCGTGGTGCGTAGCCTTCTGCACCGAGGCGCCTCGGTCAACCGCACCACGTGCACCAACTC
CACGCCGCTGCGCGCCGCCTGCTTCAACGGCCACCTGGACGTGGTGCGCTGCCTGGTGGGCGAGCACAAG
GCGGACCTGGAGGTGGCCAATCGCCACGGCCACACGTGCCTTATGATCTCCTGCTACAAGGGCCACCGCG
AGATCGCACGCTACCTGCTGGAGCGCGGCGCACAGGTGAACCGGCGCAGCGCCAAGGGCAACACCGCCCT
GCACGACTGTGCAGAATCTGGCAGCCTGGAGATCCTACAACTGCTGCTGAGCTGCCACGCGCGCATGGAG
CGCGACGGCTATGGCATGACGCCGCTGCTGGCCGCCAGCATCACTGGGCACACCAACATCGTGGAGTACC
TCATCCAGGAACAGCCGAGCCACGAGCAGCTCTCAGGGACAGAGCTTCCTGGAGAAGGCTCCTCCCAGAT
GGCAGGAAACCACTGCTCCACCCCAGAAGATGCAGAACAGTATGAGAGCTGCTGCCCTACCAGCCGGGAA
GCGGCTGTGGAAGCTTTGGAGCTGCTGGGTGCCACCTATGTGGATAAGAAAAGGGATCTGCTTGGAGCCC
TGAAGCACTGGAGAAGGGCGATGGAACTCCGCCACCAGGGTGGAGGCTTCCTCCCTAAGCCAGAGCCCCA
GCAACTGGTTCTGGCCTATGACTATTCCAGGGAGGTGACTACGCCTCAAGAGTTGGAAGCCCTCATTACA
GATCCTGATGAGATGCGGATGCAGGCGCTGCTGATACGGGAGAGAATCTTGGGCCCCTCACATCCTGACA
CTTCATACTACATACGGTACCGGGGTGCTGTCTATGCGGACTCCGGGAATTTCGAGCGCTGTATCCGTCT
GTGGAAGTATGCCTTGGACATGCAACAAAACAACCTGGAGCCCCTGAGCCCCATGACCGCCAGCAGCTTC
CTGTCGTTTGCAGAGCTCTTCTCCTACGTGCTGCAGGACCACTCGGCCAAGGGCAACCTAGGCATGCAGC
TTGACTTTGCCGACCTCATAGGTGTGCTCAGCAAAGGGGTTCGGGAAGTGGAACGGGCTCTGCAGCTGCC
CAAGGAACCGGATGACTCCGCACAGTTCACCAAAGCCATTGCCATCATCCTCCACCTTCTGTACCTATTG
GAGAAGGTGGAGTGCACCCCCAGGCAGGAACACCTCAAGCACCAGACAGTCTATCGCCTGCTCAAGTGTG
CCCCTCGTGGCAAGAACGGCTTCACCCCACTACACATGGCGGTGGACAAGGAGACCACCAACGTGGGCCA
GTATCATGTGGGCGTCTTCCCGTCACTGCAGGTGGTCAAGGTGCTGTTGGACTGCGGAGCAGACCCTGAC
AGCAGGGATTTCGACAACAACAGCCCACTGCACATCGCTGCCCAGAACAATTGCCCGGCCATCATGGATG
CTCTCATCGAAGCTGGGGCCCACATGGACGCCACCAATACCTTCAAAAAGACTGCCTATGAGCTGCTGGA
CTCGAAGCTGTTGGCCAAGAGCACCGTGCAGCCCTTCAATTATGTGACACTGCAGTGCCTAGCTGCCCGT
GCCCTGGACAGGAACAAGGTCCCTTATAAGGGCTTCATCCCAGAGGAGCTGGAGGCCTTCATCCAGTTGC
AC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216550 representing NM_176829
Red=Cloning site Green=Tags(s)

MDLHTAVYNAAHDGKLPLLQKLLASRGREELEELLGEVAGGGTPLLIAARRGHLDVVEYLVDHCGASVEA
SGSVHFDGETIEGAPPLWAASAAGHLAVVRSLLHRGASVNRTTCTNSTPLRAACFNGHLDVVRCLVGEHK
ADLEVANRHGHTCLMISCYKGHREIARYLLERGAQVNRRSAKGNTALHDCAESGSLEILQLLLSCHARME
RDGYGMTPLLAASITGHTNIVEYLIQEQPSHEQLSGTELPGEGSSQMAGNHCSTPEDAEQYESCCPTSRE
AAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGGFLPKPEPQQLVLAYDYSREVTTPQELEALIT
DPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQQNNLEPLSPMTASSF
LSFAELFSYVLQDHSAKGNLGMQLDFADLIGVLSKGVREVERALQLPKEPDDSAQFTKAIAIILHLLYLL
EKVECTPRQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKETTNVGQYHVGVFPSLQVVKVLLDCGADPD
SRDFDNNSPLHIAAQNNCPAIMDALIEAGAHMDATNTFKKTAYELLDSKLLAKSTVQPFNYVTLQCLAAR
ALDRNKVPYKGFIPEELEAFIQLH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_176829
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176829.2, NP_789799.1
RefSeq Size 3274 bp
RefSeq ORF 1965 bp
Locus ID 216622
UniProt ID Q8C0T1
Cytogenetics 11 A3.3
Summary Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. May participate in antiinflammatory signaling via its interaction with PTGER4 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_176829" in other vectors (4)
SKU Description Size Price
MC220200 4931440F15Rik (untagged) - Mouse RIKEN cDNA 4931440F15 gene (4931440F15Rik), (10ug) 10 ug
$671.00
MR216550 4931440F15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4931440F15 gene (4931440F15Rik) 10 ug
$289.00 $610.00
MR216550L3 Lenti ORF clone of 4931440F15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4931440F15 gene (4931440F15Rik) 10 ug
$910.00
MR216550L4 Lenti ORF clone of 4931440F15Rik (mGFP-tagged) - Mouse RIKEN cDNA 4931440F15 gene (4931440F15Rik) 10 ug
$910.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.