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Zfp827 (NM_178267) Mouse Tagged ORF Clone

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SKU
MG216523
Zfp827 (tGFP-tagged) - Mouse zinc finger protein 827 (Zfp827), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,139.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp827
Synonyms 2810449M09Rik; D630040G17Rik; Znf827
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216523 representing NM_178267
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAGGAGGAAGCAGGAGCAGCCCAAGCGCCTTCCCTCACATGTTAGTAGGCAGGACGAGGCGGAGG
GAGATTTCAGCGAAGGAGAGCAGTGGTACGGAAACTCCTCTGAGACTCCGTCAGAAGCGTCCTATGGGGA
AGTCCAAGAGAACTATAAGCTGTCCCTGGAGGACCGGATCCAAGAGCAGTCCACATCCCCCGACACCTCC
CTGGGCAGTGCCACACCCAGCAGCCACACGCTGGAGTTGGTGGCACTTGATGGTGAGGTTCTCCGAGACT
CGCTGCAGTGTCAAGGTCACCTTTCACCCGGAGTGTCCTCTGTGTGCGATGATGACCCACCTAGTTCCAA
TAAACCTCTGAGCAGCAATTTGAGGCGGCTGCTGGAGGCCGGGTCCCTCAAACTCGATGGCACAGCCAAT
GGCAGGGTGGAGTCTCCCGTGAATGTTGGCCCCAGTCTGTCATTTTCCCCTCCGTCCCACCATGCCCAGC
AACTCAGTGTCCTGGCCAGGAAGCTGGCGGAGAAGCAGGACCAGAGTGACCAATTCACCCCAAGTAACCG
TTTCATATGGAATCAAGGTAAGTGGTTGCCGAACTCAACCACCACCTGTGGCCTGTCCCCTGACTCCGCC
ATCCTCAAACTGAAAGCCGCAGCCAATGCTGTTCTGCAGGACAAATCTCTGTCCCGGACTGAGGAGAGCC
TGAGGTTTGAGTCTTTTTCCTCCCCGTTCAGCTCGCAGTCTGCCAGCTCCACCTTGGCGGCCTTGTCTAA
GAAAGTGAGCGAGAGAAGTCTGACTCCGGGTCAGGAGCACCCGCCTCCTGCCAGCTCTTTCCTGTCATTG
GCCTCCATGACCTCCTCCGCAGCCCTGCTGAAAGAAGTGGCTGCCAGGGCCGCGGGTAGCCTTCTGGCTG
AGAAGTCATCTTTATTGCCTGATGACCCTCTACCACTGCCGTCTTCTGAGAAGAAGCCAGAAAAAGTCAC
CCCGCCCCCTCCCCCACCTCCTCCGACAGCGCAGCCGCCTCAGTCCTTGGAATTATTGCTACTTCCAGTT
TCCAAGGGAAGAGCCTCCAAGCCCTCCAATTCAGCCCCAGAAGAAGAAAGCGGGAAGCCATTCCAATGTC
CAATATGTGGTTTGGTTATAAAAAGGAAGAGTTACTGGAAGCGGCACATGGTGATTCACACGGGCTTAAA
GAGTCATCAGTGTCCACTCTGTCCATTCCGGTGTGCTCGCAAGGACAATCTCAAATCCCACATGAAGGTT
CATCAGCATCAGGACCGGGGAGAGACTTTTCAGTGCCAGCTGTGCCCTTTCACATCCTCACGCCACTTCA
GCCTGAAACTGCACATGCGTTGCCATCAGCACTTCCTGAGGACAGAGGCCAAGGTGAAGGAGGAGATCCC
AGACCCAGATGTCAAGGGATCTCCCCACCTCAGTGACAGTGGCTGCCTGGGGCAGCAGAGGGAAGGAGGA
GGGACAGAGCTAGTGGGGACCGTGATGACGTCTAACACGCCAGAGAGGACTGGCCAGGGTGGGGCTGGCG
TTGCACCTTTGCTGGTGAAGGAGGAGCCCAAGGAAGATAACGGCCTGCCCACCTCCTTTACCTTGAATGC
TGCCGACAGGCCCGCCAACCACACAAAGCTGAAAGACCCCTCCGAGTATGTGTCCAACAGTGCAGCAGTA
TTGTTCAGCCAGGACATCTCGGTTAAGATGGCCTCCGATTTTCTCATGAAGCTGTCAGCTGCAAACCAGA
AGGAGCCTATGAATCTTAATTTTAAAGTGAAGGAGGAGCCCAAGGAAGAGGAGTCCCTGAGCATGCCTTT
GCCTCGCTCCAGCTACGTGTTCAGCCCGGAACCGGAAGTGTCTACCCCAAGCGTCTCCGAGGACCCACTT
ACACCCCAGGAAGGCAAGGGGAGTGTGTTAAGGCGGGACATGTCGGCCAAGGCTGCCTCAGAACTTCTCA
TGAAACTCTCAGCGGAAAGCTACAAGGAGACGCAGGCGGTGACGGTTAAAGAAGAACCCATGGAGGTGGA
CATCCAGGACTCCCCAGCCTCCATATCACCCAGCCGGAACATTGGCTACAGCACTTTAATGGGGCGAGAG
AAAACTGAACCCTTACAGAAGCTGCCAGAAGGTCGAGTTCCCCCAGAGAGAAATCTCTTCAGCCAGGACA
TCTCTGTGAAGATGGCTTCTGAGCTGCTTTTTCAGCTCTCGGAAAAAGTGAGCAAAGAACACAATCACAC
GAAAGAAAACGCAATGCGGACAACCACCAGCCCTTTCTTTTCAGAAGACACATTTAGACAATCACCATTC
ACCTCCAATTCAAAAGATCTGCTGCCTGGTGAATCTGTGCTGCACGGAAGAGTGTCTGCTCCAGAGACCG
AGAAGATTGTCCTTGAGGCAGGAAACGGACTGCCATCCTGGAAATTCAATGACCAGCTCTTCCCTTGCGA
TGTGTGTGGGAAAGTGTTCGGACGACAGCAGACATTGTCTCGACACCTCTCATTGCACACAGAGGAAAGA
AAATACAAATGCCACTTGTGCCCCTATGCTGCTAAGTGCCGTGCGAACCTGAACCAGCATCTGACCGTGC
ACTCTGTGAAGCTCGTGAGTACCGACACCGAGGACATAGTCAGTGCTGTCACTTCTGAGGGCAGTGATGG
GAAGAAACACCCGTATTACTACAGTTGCCACGTGTGTGGATTTGAGACGGAGCTCAATGTTCAGTTTGTA
AGCCACATGTCTCTCCATGTGGACAAGGAACAGTGGATGTTTTCCATCTGCTGCACGGCCTGCGACTTTG
TCACCATGGAGGAAGCAGAGATAAAGACTCACATTGGCACCAAGCACACAGGGGATGATAGGAAGACCCC
CAGTGAATCGAACAGCCCCTCTTCATCCTCCCTGTCAACTCTGAGTGATTCAGCCAATGGCAAAGATGAC
TCAGACAGCTCCCAGAAAAACAAGGGCGGGAACAACTTGCTGGTCATCTCTGTGGTGCCCGGGAGCCAGC
CCTCACTGAACAACGAGGAAAAGCCAGAGAAAGGGTTCGAATGCGTTTTTTGCAACTTTGTCTGCAAGAC
GAAGAATATGTTTGAGCGCCATCTGCAAATCCACCTCATCACCCGGATGTTTGAGTGTGATGTGTGCCAC
AAGTTCATGAAGACTCCCGAACAGCTGCTGGAGCACAAGAAATGCCACACTGTCCCCACCGGTGGGCTCA
ACTCAGGACAGTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216523 representing NM_178267
Red=Cloning site Green=Tags(s)

MPRRKQEQPKRLPSHVSRQDEAEGDFSEGEQWYGNSSETPSEASYGEVQENYKLSLEDRIQEQSTSPDTS
LGSATPSSHTLELVALDGEVLRDSLQCQGHLSPGVSSVCDDDPPSSNKPLSSNLRRLLEAGSLKLDGTAN
GRVESPVNVGPSLSFSPPSHHAQQLSVLARKLAEKQDQSDQFTPSNRFIWNQGKWLPNSTTTCGLSPDSA
ILKLKAAANAVLQDKSLSRTEESLRFESFSSPFSSQSASSTLAALSKKVSERSLTPGQEHPPPASSFLSL
ASMTSSAALLKEVAARAAGSLLAEKSSLLPDDPLPLPSSEKKPEKVTPPPPPPPPTAQPPQSLELLLLPV
SKGRASKPSNSAPEEESGKPFQCPICGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKV
HQHQDRGETFQCQLCPFTSSRHFSLKLHMRCHQHFLRTEAKVKEEIPDPDVKGSPHLSDSGCLGQQREGG
GTELVGTVMTSNTPERTGQGGAGVAPLLVKEEPKEDNGLPTSFTLNAADRPANHTKLKDPSEYVSNSAAV
LFSQDISVKMASDFLMKLSAANQKEPMNLNFKVKEEPKEEESLSMPLPRSSYVFSPEPEVSTPSVSEDPL
TPQEGKGSVLRRDMSAKAASELLMKLSAESYKETQAVTVKEEPMEVDIQDSPASISPSRNIGYSTLMGRE
KTEPLQKLPEGRVPPERNLFSQDISVKMASELLFQLSEKVSKEHNHTKENAMRTTTSPFFSEDTFRQSPF
TSNSKDLLPGESVLHGRVSAPETEKIVLEAGNGLPSWKFNDQLFPCDVCGKVFGRQQTLSRHLSLHTEER
KYKCHLCPYAAKCRANLNQHLTVHSVKLVSTDTEDIVSAVTSEGSDGKKHPYYYSCHVCGFETELNVQFV
SHMSLHVDKEQWMFSICCTACDFVTMEEAEIKTHIGTKHTGDDRKTPSESNSPSSSSLSTLSDSANGKDD
SDSSQKNKGGNNLLVISVVPGSQPSLNNEEKPEKGFECVFCNFVCKTKNMFERHLQIHLITRMFECDVCH
KFMKTPEQLLEHKKCHTVPTGGLNSGQW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178267
ORF Size 3234 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178267.3, NP_839998.2
RefSeq Size 7625 bp
RefSeq ORF 3237 bp
Locus ID 622675
UniProt ID Q505G8
Cytogenetics 8 C1
Summary May be involved in transcriptional regulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178267" in other vectors (4)
SKU Description Size Price
MC223465 Zfp827 (untagged) - Mouse zinc finger protein 827 (Zfp827), (10ug) 10 ug
$1,033.00
MR216523 Zfp827 (Myc-DDK-tagged) - Mouse zinc finger protein 827 (Zfp827) 10 ug
$589.00 $939.00
MR216523L3 Lenti ORF clone of Zfp827 (Myc-DDK-tagged) - Mouse zinc finger protein 827 (Zfp827) 10 ug
$1,239.00
MR216523L4 Lenti ORF clone of Zfp827 (mGFP-tagged) - Mouse zinc finger protein 827 (Zfp827) 10 ug
$1,239.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.