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Znrf1 (NM_133206) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216517
Znrf1 (tGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Znrf1
Synonyms B830022L21Rik; nin283; Rnf42; Zrfp1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216517 representing NM_133206
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGGCAAGCAGAGCACGGCGGCCCGCTCTCGGGGCCCCTTCCCGGGGGTCTCTACGGATGACAGCG
CCGTGCCGCCGCCGGGAGGGGCGCCCCACTTTGGGCACTACCGGACGGGCGGCGGGGCGATGGGGCTGCG
CAGCCGCTCGGTCAGCTCGGTGGCGGGCATGGGCATGGACCCCAGCACGGCCGGAGGGGTGCCCTTTAGT
CTCTACACCCCCGCCTCCCGCGGGACCGGCGACTCCGAGAGGGCGCCGGGCGGCGGAGGGTCCACGTCGG
ACTCCACCTATGCCCACGGCAATGGTTACCAAGAGACCGGCGGCGGTCACCATAGAGACGGGATGCTGTA
CCTGGGCTCCCGAGCCTCGCTGGCGGATGCTCTACCTCTGCACATCGCACCCAGGTGGTTCAGCTCGCAC
AGTGGTTTCAAGTGCCCCATTTGTTCCAAGTCTGTGGCTTCCGATGAGATGGAAATGCACTTTATAATGT
GTCTGAGCAAGCCTCGCCTGTCCTACAATGATGATGTGCTGACTAAAGATGCGGGTGAGTGTGTGATCTG
CCTGGAGGAGCTGCTTCAGGGGGACACGATAGCCAGGCTGCCTTGCCTGTGCATCTATCACAAAAGCTGC
ATAGACTCATGGTTTGAAGTGAACAGATCTTGTCCAGAGCACCCTGCTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216517 representing NM_133206
Red=Cloning site Green=Tags(s)

MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMDPSTAGGVPFS
LYTPASRGTGDSERAPGGGGSTSDSTYAHGNGYQETGGGHHRDGMLYLGSRASLADALPLHIAPRWFSSH
SGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSC
IDSWFEVNRSCPEHPAD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133206
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133206.4
RefSeq Size 5562 bp
RefSeq ORF 684 bp
Locus ID 170737
UniProt ID Q91V17
Cytogenetics 8 E1
Summary E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes Wallerian degeneration, a neurodegeneration disorder, by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133206" in other vectors (6)
SKU Description Size Price
MC200768 Znrf1 (untagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1, (10ug) 10 ug
$300.00
MC218756 Znrf1 (untagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1, (10ug) 10 ug
$330.00
MG202714 Znrf1 (tGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1) 10 ug
$500.00
MR216517 Znrf1 (Myc-DDK-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 10 ug
$289.00 $300.00
MR216517L3 Lenti ORF clone of Znrf1 (Myc-DDK-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 10 ug
$600.00
MR216517L4 Lenti ORF clone of Znrf1 (mGFP-tagged) - Mouse zinc and ring finger 1 (Znrf1), transcript variant 1 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.