Arap1 (NM_001040111) Mouse Tagged ORF Clone

CAT#: MG216506

Arap1 (tGFP-tagged) - Mouse ArfGAP with RhoGAP domain ankyrin repeat and PH domain 1 (Arap1) transcript variant 3, (10ug)

Product Images

Circular map for MG216506

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arap1
• Synonyms: 2410002L19Rik; Centd2; mKIAA0782
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216506 representing NM_001040111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGGTTACGATGCCGCACTGTCTGTAGCTGAGTGGCTGCGGGCACTTCACCTGGAGCAGTACA
  CGGCACTCTTTGAGCAGCACGGACTGGTGTGGGCCACCGAGTGCCAAGGCCTCAGCGATGCTGGCCTGCT
  GGACATGGGCATGCACCTCCCCGGCCACCGCCGCCGCATCCTGGCTGGTCTTCACCGCGCCCATGCACCC
  CCGGTCCCTCTGCCTCGCCCCGCCCCACGGCCCGTGCCGATGAAGCGACACATCTTCCGTTCACCACCTG
  TGCCCGTCACTCCACCAGAGCCGCCGCCCACAGCTGGAGAGGATGAGGGTCTGCCTGCCGCTCCACCTAT
  CCCACCCCGCAGGAGCTGCCTCCCACCTGCCTGCTTCACTCCGACATCCACAGCCGCCCCAGATCCTGTG
  CTGCCCCCACTGCCTGCCAAGAGACACTTGGTAGAATCCAGCGTTCCTCCTGTCCCTCCTCGAACCGGAC
  CCCCCTACCCACAGGCCAGCCTTCTTGCTAAGGAGGAGTTACTGCTGCCATCAGTGTCACCCCGGTCCCA
  ACCAGAGCCTGCAGAGACTCCGTCCACCCTCCTGCCTGCCTTTCCCCAGGGGCCCCTGCAGCCTCCGTCT
  CCTCCTCCCTGTCCTCCTGTGATCCCTCCCAAGCCTCCTCGCCTGCTCCCTGAGTTTGACGACTCTGACT
  ATGATGATGTCCCGGAGGAGGGGCCAGGGGCCCCAGCCAGTGTGATGACCAAGGAGGAGCCCCTGCCGAG
  CCGAGTCCCACGGGCTGTGCGTGTGGCTAGTCTGCTGAGCGAGGGGGAGGAGCTGTCTGGGGATGATGCG
  GAAGATGACGATGACCATGCCTATGAGGGTATCCCCAATGGTGGGTGGCCAACGAGTGGCCTGAATCCAC
  CCTTACGCAGCCTGATCCCTGATCTCCCACTGCACCCCATGGATGAGTTGCCTGGGGGTCCCACCCCCAT
  CACACCTGTCATCAAGGCTGGCTGGCTGGACAAGAACCCACCACAGGGGTCTTATATCTATCAGAAGCGA
  TGGGTGAGACTGGACGCTGATTACCTGCGATACTTTGACAGTAACAAGGACGCCTACTCTAAGCGCTTTG
  TTCCTGTGGCCTGCATCTGCCGAGTAGCTCCTATTGGAGACCAGAAGTTTGAAGTGATCACAAATAATCG
  GACCTTCGCCTTCCGGGCAGAGAGTGATGTGGAGCGGAACGAGTGGATGCAGGCCCTGCAGCAGGCAGTA
  GTTGAGCATCGAGCCCGTTTTCGGCTTTCTAGTGCTTCTGTGTTGGGAGTCCGAGGCTCCGAGCAGCCTG
  ACCGTGCTGGCAGCCTGGAGCTACGAGGCTTCAAGAATAAGCTTTACGTGGCTGTGACTGGGGACAAAGT
  ACAGCTCTATAAGAATCTGGAGGAATTCCATCTGGGCATTGGTATCACCTTTATCGACATGAATGTGGGC
  AATGTCAAGGAAGTGGATCGGCGCAGCTTTGACCTCACTACCCCCTACCGCATCTTCAGCTTCTCGGCTG
  ATTCAGAGTTAGAGAAGGAACAGTGGCTGGAGGCCATGCAGGGAGCCATCGCAGAGGCCCTATCTACCTC
  AGAGGTGGCTGAGCGCATCTGGGCCGCAGCCCCCAACAGGTTCTGTGCCGACTGTGGGGCCGCCCAGCCT
  GACTGGGCCTCCATCAACCTCTGCGTTGTCATCTGCAAGCGCTGTGCAGGGGAGCACCGCGGCCTGGGTG
  CAGGAGTGTCCAAGGTGCGGAGTTTGAAGATGGACAGGAAGGTGTGGACAGAAGCACTCATCCAGCTCTT
  CTTACATCTGGGCAATGGCCCCGGGAACCACTTCTGGGCTGCTAATGTGCCTCCTAGTGAGGCCCTGGAG
  CCCAGCAGCAGCCCTGGTGCCCGGCGGTATCACCTGGAGGCCAAGTACAGAGAGGGCAAGTACCGCCGCT
  ACCATCCGCTCTTTGGCAACCAAGAGGAACTGGACAAGGCCCTGTGTGCTGCAGTTACTACCACTGACCT
  GGCTGAGACCCAGGCACTCCTGGGCTGTGGGGCTGGGGTCAGCTGCTTCTCAGGGGACCCAGCAGCTCCC
  ACACCCCTGGCTCTTGCTGAGCAGGCCGGACAGACTCTGCAGATGGAGTTTCTACGGAATAATCAGAGTA
  CAGAGGTCCCTCGGTTGGACTCAGTGAAGCCCTTGGAAAAGCACTACTCCGTTACCCTGCCAACTGTGAG
  CCACAGCGGCTTCCTGTACAAAACTGCTTCTGCCGGCAAACCTCTGCAGGACCGCCGTGCCCGGGAAGAG
  TTCAGCCGGCGCTGGTGTGTCCTGAGTGATGGGGTCCTGAGCTACTATGAAAACGAACGGGCAGTGACAC
  CCAATGGGGAGATTCGGGCCAGCGAGATAGTATGCCTAGCAGTTTCCCCTCTGGACACCCATGGCTTTGA
  GCACACCTTTGAGGTGTACACAGAGGGAGAACGGCTGTACCTGTTTGGGCTGGAGAATGCAGAGCTGGCT
  CATGAGTGGGTCAAGTGCATTGCCAAGGCGTTCGTGCCTCCCCTGGCTGAGGACCTGCTAGCCCGGGACT
  TTGAACGTCTTGGGCGCCTACCCTGTAAAGCTGGCCTGAGCCTTCAGCAGGCTCAGGAAGGCTGGTTTGC
  CTTGACTGGCTCTGAGCTCCGGGCTGTCTTCCCAGAGGGGCCCTGGGAAGAGCCGCTGCAGCTCCGGAAA
  CTGCAAGAGCTTTCTATCCAAGGAGACAGCGAGAACCAAGTGCTGGTGCTGGTGGAGCGGAGGAGGACAC
  TGTACATCCAGGGTGAGCGGCGGCTGGACTTCATGGCTTGGCTAGGGGTCATCCAGAAAGCAGCAGCCAG
  CTTGGGAGACACACTATCAGAACAACAGCTTGGGGACTCGGACATCCCAGTGATTGTGTACCGCTGTGTG
  GACTACATCACGCAGTGTGGCTTGACCTCAGAGGGTATCTATCGAAAGTGTGGTCAGACCTCCAAGACTC
  AGAGACTGCTAGACAGCCTCCGGCAGGACGCACGCTCTGTGCACCTAAAGGAGGGAGAACAGCACGTGGA
  CGACGTCTCCTCTGCACTCAAACGCTTCCTCAGGGACCTGCCCGATGGGCTCTTCACGCGTGCGCAGCGC
  CTGGCCTGGCTGGAGGCCTCTGAGATCGAGGATGAGGAAGAAAAGATCTCCAGGTATCGAGAGCTCTTGG
  TGCATCTGCCCCCTGTCAACCGGGCCACTGTGAAGGCCCTTATCAGCCATCTGTACTGTGTACAGTGCTT
  CTCAGACACAAACCAAATGAACACACACAACCTGGCTATCGTGTTTGGGCCTACACTCTTCCAGACAGAC
  GGGCAGGACTACAAGGCCGGCAAGGTGGTGGAAGACCTCATCAACCACTACGTGGTGGTGTTCAGTGTGG
  ACGAGGAGGAGCTGAGGAAGCAGAGGGAGGAAGTCACGGCCATCGTGAAGATGCGAGTGGCTGGCACTGC
  CAGTGGGACCCAGCATGCTGGCGACTTCATCTGCACAGTCTACCTGGAGGAGAAGAAGGTGGAGACTGAA
  CAGCATGTTAAGATCCCAGCATCCATGACTGCAGAGGAGCTTACTCTGGAGATTCTGGACCGCCGCAATG
  TGAGCATCAGGGAGAAGGACTACTGGACTTGCTTTGAGGTCAACGAGAAGGAGGAGGCAGAGCGCCCGCT
  GCACTTTGCAGAGAAGGTGCTGCCCATTGTCCATGGGCTGGGCATAGACAGCCATCTGGTGGTGAAGAAG
  TACCAGTCCATGGAGGCCATGCTGTTGTACTTGGCCAGCCGTGTGGGTGACACCAAGCATGGTATGATGA
  AGTTCCGTGAAGACCGCAGCCTCCTGGGTCTGGGGCTACCTTCGGGTGGCTTCCACGATCGCTACTTCAT
  TCTCAACAGCAGCTGCCTACGGCTCTACAAGGAGGTCCGGAGTCACCGGCCTGAGAAGGAGTGGCCTGTC
  AAGAGCCTCAAAGTCTACCTGGGTGTAAAGAAGAAACTGCGGCCACCTACTTGCTGGGGCTTCACGGTGG
  TGCACGAGACAGAAAAGCACGAGAAGCAGCAGTGGTACCTCTGCTGTGACACGCAGATGGAACTTCGAGA
  GTGGTTTGCCACCTTCCTCTCTGTGCAGCACGATGGCCTGGTGTGGCCCTCCGAGCCATCTCGAGTGTCC
  CGGGCAGTGCCTGAGGTCCGGATGGGCAGTGTATCGCTGATCCCTCTACGAGGCAGTGAAAATGAAATGC
  GCCGGAGTGTGGCAGCCTTCACTGCTGACCCCCTTTCCCTTCTCCGCCATGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216506 representing NM_001040111
  Red=Cloning site Green=Tags(s)
  
  MAEGYDAALSVAEWLRALHLEQYTALFEQHGLVWATECQGLSDAGLLDMGMHLPGHRRRILAGLHRAHAP
  PVPLPRPAPRPVPMKRHIFRSPPVPVTPPEPPPTAGEDEGLPAAPPIPPRRSCLPPACFTPTSTAAPDPV
  LPPLPAKRHLVESSVPPVPPRTGPPYPQASLLAKEELLLPSVSPRSQPEPAETPSTLLPAFPQGPLQPPS
  PPPCPPVIPPKPPRLLPEFDDSDYDDVPEEGPGAPASVMTKEEPLPSRVPRAVRVASLLSEGEELSGDDA
  EDDDDHAYEGIPNGGWPTSGLNPPLRSLIPDLPLHPMDELPGGPTPITPVIKAGWLDKNPPQGSYIYQKR
  WVRLDADYLRYFDSNKDAYSKRFVPVACICRVAPIGDQKFEVITNNRTFAFRAESDVERNEWMQALQQAV
  VEHRARFRLSSASVLGVRGSEQPDRAGSLELRGFKNKLYVAVTGDKVQLYKNLEEFHLGIGITFIDMNVG
  NVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAERIWAAAPNRFCADCGAAQP
  DWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTEALIQLFLHLGNGPGNHFWAANVPPSEALE
  PSSSPGARRYHLEAKYREGKYRRYHPLFGNQEELDKALCAAVTTTDLAETQALLGCGAGVSCFSGDPAAP
  TPLALAEQAGQTLQMEFLRNNQSTEVPRLDSVKPLEKHYSVTLPTVSHSGFLYKTASAGKPLQDRRAREE
  FSRRWCVLSDGVLSYYENERAVTPNGEIRASEIVCLAVSPLDTHGFEHTFEVYTEGERLYLFGLENAELA
  HEWVKCIAKAFVPPLAEDLLARDFERLGRLPCKAGLSLQQAQEGWFALTGSELRAVFPEGPWEEPLQLRK
  LQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMAWLGVIQKAAASLGDTLSEQQLGDSDIPVIVYRCV
  DYITQCGLTSEGIYRKCGQTSKTQRLLDSLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQR
  LAWLEASEIEDEEEKISRYRELLVHLPPVNRATVKALISHLYCVQCFSDTNQMNTHNLAIVFGPTLFQTD
  GQDYKAGKVVEDLINHYVVVFSVDEEELRKQREEVTAIVKMRVAGTASGTQHAGDFICTVYLEEKKVETE
  QHVKIPASMTAEELTLEILDRRNVSIREKDYWTCFEVNEKEEAERPLHFAEKVLPIVHGLGIDSHLVVKK
  YQSMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPV
  KSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLSVQHDGLVWPSEPSRVS
  RAVPEVRMGSVSLIPLRGSENEMRRSVAAFTADPLSLLRHV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040111
• ORF Size: 4323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040111.1, NP_001035200.1
• RefSeq Size: 5837 bp
• RefSeq ORF: 4326 bp
• Locus ID: 69710
• UniProt ID: Q4LDD4
• Cytogenetics: 7 E2
• Gene Summary: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).[UniProtKB/Swiss-Prot Function]