Naaa (NM_001163687) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216501
Naaa (tGFP-tagged) - Mouse N-acylethanolamine acid amidase (Naaa) transcript variant 2, (10ug)
  $703.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Naaa
Synonyms 2210023K21Rik; 3830414F09Rik; Asahl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216501 representing NM_001163687
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCCTAGCCACCAGGGCCGCGTGCCATGGGGCACACCTGGCCCTGGCTCTGTTGCTGCTACTGT
CGCTGTCCGGTCCCTGGCTGTCGGCTGTCGTCCCCGGGACTCCGCCTCTCTTCAACGTCAGTCTGGACGT
GGCCCCGGAGCAGCGCTGGCTGCCGATGCTGCGGCACTACGACCCAGACTTCTTGCGCACCGCGGTGGCG
CAGGTCATTGGCGACAGGGTTCCCCAGTGGGTACTCGGGATGGTCGGAGAAATTGTGTCGAAGGTGGAGA
GCTTCCTGCCTCAGCCCTTCACCGACGAGATCCGCAGCATCTGTGACTCGCTCAACCTCAGCCTGGCTGA
CGGTATCTTGGTCAACCTGGCCTACGAGGCTTCCGCATTCTGCACCAGTATTGTGGCCCAAGACTCCCAA
GGCCGTATTTACCACGGGCGGAACCTGGACTATCCTTTTGGAAAAATCTTACGAAAGCTGACAGCGGACG
TGCAGTTTATAAAGAATGGGCAGATTGCATTCACTGGGACCACTTTTGTTGGCTATGTAGGACTGTGGAC
AGGCCAAAGTCCACACAAGTTTACAATTTCTGGTGATGAACGAGATAAAGGCTGGTGGTGGGAGAATATG
ATCGCGGCGCTTTCTCTGGGACACTCCCCCATCAGCTGGCTTATCCGCAAAACCCTGAGTGAGTCAGAAA
GCTTCGAAGCAGCTGTCTACACGCTGGCCAAGACTCCCCTCATTGCTGACGTTTATTACATTGTTGGGGG
TACATCGCCCAAGGAGGGAGTAGTCATCACCAGGGACCGAGGTGGCCCGGCAGACATTTGGCCTCTTGAC
CCTCTGAATGGAGAGTGGTTCCGAGTTGAGACAAATTATGACCATTGGAAGCCTGCACCCAAGGTGGATG
ACCGAAGAACACCAGCCATCAAAGCCCTAAATGCTACAGGGCAAGCTCACCTCAATCTGGAGACCCTCTT
CCAGGTTTTATCCCTGTTTCCTGTTTATAACAACTACACAATTTATACTACAGTGATGAGCGCTGCCGAG
CCCGACAAGTACCTGACCATGATCAGAAACCCGAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216501 representing NM_001163687
Red=Cloning site Green=Tags(s)

MGTLATRAACHGAHLALALLLLLSLSGPWLSAVVPGTPPLFNVSLDVAPEQRWLPMLRHYDPDFLRTAVA
QVIGDRVPQWVLGMVGEIVSKVESFLPQPFTDEIRSICDSLNLSLADGILVNLAYEASAFCTSIVAQDSQ
GRIYHGRNLDYPFGKILRKLTADVQFIKNGQIAFTGTTFVGYVGLWTGQSPHKFTISGDERDKGWWWENM
IAALSLGHSPISWLIRKTLSESESFEAAVYTLAKTPLIADVYYIVGGTSPKEGVVITRDRGGPADIWPLD
PLNGEWFRVETNYDHWKPAPKVDDRRTPAIKALNATGQAHLNLETLFQVLSLFPVYNNYTIYTTVMSAAE
PDKYLTMIRNPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163687
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163687.1, NP_001157159.1
RefSeq Size 2418 bp
RefSeq ORF 1083 bp
Locus ID 67111
UniProt ID Q9D7V9
Cytogenetics 5 E2
Summary Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-stearoylethanolamine > N-oleoylethanolamine > N-linoleoylethanolamine > N-arachidonoylethanolamine.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163687" in other vectors (4)
SKU Description Size Price
MC210488 Naaa (untagged) - Mouse N-acylethanolamine acid amidase (Naaa), transcript variant 2, (10ug) 10 ug
$503.00
MR216501 Naaa (Myc-DDK-tagged) - Mouse N-acylethanolamine acid amidase (Naaa), transcript variant 2 10 ug
$503.00
MR216501L3 Lenti ORF clone of Naaa (Myc-DDK-tagged) - Mouse N-acylethanolamine acid amidase (Naaa), transcript variant 2 10 ug
$803.00
MR216501L4 Lenti ORF clone of Naaa (mGFP-tagged) - Mouse N-acylethanolamine acid amidase (Naaa), transcript variant 2 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.