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Mdm1 (NM_001162904) Mouse Tagged ORF Clone

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SKU
MG216494
Mdm1 (tGFP-tagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $857.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mdm1
Synonyms Arrd2; Mdm-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216494 representing NM_001162904
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGTGCGCTTCAAGGGGCTGAGTGAATACCAGAGAAACTTCCTGTGGAAAAAGTCCTATTTGTCAG
AGTCTTATAATCCCTCAGTGGGACAAAAGTACTCATGGGCAGGACTTAGATCGGATCAGTTGGGGATCAC
GAAAGAACCAGGTTTTATTTCAAAAAGAAGAGTTCCCTACCATGACCCTCAGATTTCAAAATACCTGGAG
TGGAACGGAACCGTCAGAAAGAAGGATACGCTTGTCCCACCAGAACCCCAGGCCTTTGGAACGCCAAAGC
CACAAGAGGCTGAGCAAGGAGAAGATGCCAATCAAGAAGCAGTTCTCTCACTAGAGGCCTCCAGGGTTCC
CAAGAGAACTCGGTCTCATTCTGCGGACTCGAGAGCTGAAGGGGTTTCAGACACTGTGGAAAAGCACCAG
GGTGTCACGAGAAGCCATGCGCCAGTTAGCGCGGATGTGGAGCTGAGACCTTCCAGCAAACAACCTCTCT
CCCAGAGCATAGATCCCAGGTTGGATAGGCATCTTCGTAAGAAAGCTGGATTGGCCGTTGTTCCCACGAA
TAATGCCTTGAGAAATTCTGAATACCAAAGGCAGTTTGTTTGGAAGACTTCTAAAGAAAGCGCTCCAGTG
TTTGCATCCAATCAGGTTTTCCGTAATAAAAGCCAAATTATTCCACAGTTCCAAGGCAATACATTCACCC
ACGAGACTGAATACAAGCGAAATTTCAAGGGTTTAACTCCAGTGAAGGAACCAAAGTCAAGAGAGTATTT
GAAAGGAAACAGCAGTCTGGAGATGCTGACTCCAGTAAAGAAGGCAGATGAGCCTTTAGACTTAGAAGTA
GACATGGCGTCGGAAGACTCAGACCAGTCTGTAAAGAAGCCTGCTTCATGGAGACACCAAAGGCTTGGAA
AAGTGAATTCTGAATATAGAGCAAAGTTCCTGAGCCCAGCCCAGTATTTCTATAAAGCTGGAGCTTGGAC
CCGGGTGAAGGAGAACCTGTCAAACCAGGGTTCTCTAAATGCCATGTGGTATGCAGAGGTTAAGGAGCTC
CGAGAAAAGGCCGAATCTTACAGGAAGCGAGTTCAGGGGACACATTTTTCTCGGGACCACCTGAACCAGA
TTATGTCGGACAGCAACTGCTGTTGGGACGTCTCCTCAGTCACAAGCTCGGAAGGCACCGTCAGTAGCAA
CATCCGAGCACTGGATCTTGCTGGAGACCTTACAAACCACAGGACCCCCCAGAAACACCCTCCTACCAAA
CTAGAAGAAAGAAAAGTTGCCTCGGGAGAGCAGCCCCTGAAAAACTCCACCAGGAGACTGGAGATGCCAG
AGCCTGCCGCCTTGGTCAGGAGGAAGCTGGCTTGGGATGCTGAGGAGAGCACGAAGGAAGACACCCAGGA
GGAGCCCAGGGCGGAGGAGGACGGGAGAGAGGAGAGAGGACAGGGCAAGCAGACCTGTGCGGTAGAGCTG
GAGAAACCGGACACACAGACACCCAAGGCAGACAGACTGACAGAAGGGTCAGAGACATCTTCTGTTTCCT
CAGGGAAGGGAGGCAGGCTTCCTACACCGAGGCTGAGAGAACTCGGTATCCAGCGGACGCACCATGATCT
CACGACGCCAGCTGTTGGTGGCGCAGTCTTAGTGTCTCCATCTAAAGTGAAGCCACCAGGCCTCGAGCAG
AGGAGGAGAGCGTCCTCCCAAGATGGCTTAGAAACTCTGAAGAAAGACATTACTAAGAAAGGAAAACCCC
GTCCCATGTCTCTGTTGACTTCTCCGGCTGCTGGCATGAAGACAGTTGATCCCCTGCCTCTGCGAGAAGA
TTGTGAAGCCAATGTGCTCAGATTTGCTGAGGGTACTCTTCCTGTTTCGAAAATTTTGGACCGTCAGCCC
AGCACCCCTGGGCAGCTGCCTCCATGTGCCCCGCCTTACTGTCATCCGTCCAGCAGGATCCAGGGCCGTC
TTCGAGACCCTGAGTTTCAGCACAACATGGGAAAACCAAGGACGAACAATTTGCAGCTACATCCACACGA
TGCCTTTAATGATGAAGATGCAGATAGACTGTCTGAGATCTCTGCTCGCTCTGCAGTTTCCAGCCTCCGG
GCTTTCCAGACTCTAGCCCGAGCTCAGAAAAGAAAGGAGAATTTCTGGGGCAAGCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216494 representing NM_001162904
Red=Cloning site Green=Tags(s)

MPVRFKGLSEYQRNFLWKKSYLSESYNPSVGQKYSWAGLRSDQLGITKEPGFISKRRVPYHDPQISKYLE
WNGTVRKKDTLVPPEPQAFGTPKPQEAEQGEDANQEAVLSLEASRVPKRTRSHSADSRAEGVSDTVEKHQ
GVTRSHAPVSADVELRPSSKQPLSQSIDPRLDRHLRKKAGLAVVPTNNALRNSEYQRQFVWKTSKESAPV
FASNQVFRNKSQIIPQFQGNTFTHETEYKRNFKGLTPVKEPKSREYLKGNSSLEMLTPVKKADEPLDLEV
DMASEDSDQSVKKPASWRHQRLGKVNSEYRAKFLSPAQYFYKAGAWTRVKENLSNQGSLNAMWYAEVKEL
REKAESYRKRVQGTHFSRDHLNQIMSDSNCCWDVSSVTSSEGTVSSNIRALDLAGDLTNHRTPQKHPPTK
LEERKVASGEQPLKNSTRRLEMPEPAALVRRKLAWDAEESTKEDTQEEPRAEEDGREERGQGKQTCAVEL
EKPDTQTPKADRLTEGSETSSVSSGKGGRLPTPRLRELGIQRTHHDLTTPAVGGAVLVSPSKVKPPGLEQ
RRRASSQDGLETLKKDITKKGKPRPMSLLTSPAAGMKTVDPLPLREDCEANVLRFAEGTLPVSKILDRQP
STPGQLPPCAPPYCHPSSRIQGRLRDPEFQHNMGKPRTNNLQLHPHDAFNDEDADRLSEISARSAVSSLR
AFQTLARAQKRKENFWGKP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162904
ORF Size 2157 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162904.1, NP_001156376.1
RefSeq Size 3161 bp
RefSeq ORF 2157 bp
Locus ID 17245
UniProt ID Q9D067
Cytogenetics 10 66.65 cM
Summary Microtubule-binding protein that negatively regulates centriole duplication. Binds to and stabilizes microtubules.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001162904" in other vectors (4)
SKU Description Size Price
MC220992 Mdm1 (untagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1), transcript variant 3, (10ug) 10 ug
$724.00
MR216494 Mdm1 (Myc-DDK-tagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1), transcript variant 3 10 ug
$589.00 $657.00
MR216494L3 Lenti ORF clone of Mdm1 (Myc-DDK-tagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1), transcript variant 3 10 ug
$957.00
MR216494L4 Lenti ORF clone of Mdm1 (mGFP-tagged) - Mouse transformed mouse 3T3 cell double minute 1 (Mdm1), transcript variant 3 10 ug
$957.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.