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Kndc1 (NM_177261) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216490
Kndc1 (tGFP-tagged) - Mouse kinase non-catalytic C-lobe domain (KIND) containing 1 (Kndc1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,665.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Kndc1 or NM_177261"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kndc1
Synonyms 2410012C07Rik; B830014K08Rik; very-kind; VKIND
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216490 representing NM_177261
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCCATGGACCCAGCCTCCCGGGGCTTCTACGAGGAAGATGGCAAGGACTTGGACTTCTACGACT
TCGAGCCGCTCCCCACCCTGCCGGAGGATGAGGAAAATGTGTCTCTGGCTGACATCCTCTCCCTGCGGGA
CAGAGGCCTCAGTGAGCAGGAGGCCTGGGCTGTGTGCCTAGAGTGCAGCCTGTCCATGCGGAGTGTCGCC
CACGCAGCCATCTTCCAGACCCTGTGCATCACACCAGACACCTTGGCCTTCAACACCAGTGGAAACGTGT
GCTTCATGGAACAGCTCAGTGATGACCCCGAGGGTGCCTTTGTACCCCCAGAGTTTGACCTGACAGGAAA
CACCTTTGAGGCTCACATCTACTCCCTGGGGGCCACACTGAAGGCTGCCCTGGAGTATGTCCCAGAACCA
GAGCTGGAGCCCAAGCTGAGCACAGACCTGGAGGGACTGCTGAGCCAGATGCAGGCAGAAGACCCCAGAG
AGCGGCCGGACCTGGCGAGCATCATAGCACTGTGTGAGGAGAAGATGCAGCCTGTGTCCTCATGCCGCCT
GTGCCGCAGTCTCTCAGCCATTGGAAGAAGGGTGCTCTCCATTGAGTCCTTCGGAGCATTCCAGGAGCTC
AGCGAGAACACATGGAGGGGAAGGCCTGCTCCAAGAAACGTGGGGCCCAAGAAGATGCCAGGAGACCTCA
GCACTGACCCAGAGGCACTGTTCCCATCAAAGGGCCTACTCCAGCCTCCTGCTTCCAGGGATGCCGAGCA
GGAGGCTGGGCAGAGGCCCAGAGCCCCATCCCCCAAACCACTGCTGTCAGCCCCTGTGAGAAATGGTGAG
AACCCAGGACAGGAAGGGCTGGCTGACCTTGTCCTGGATGCCAGGTGCCCCCTGGGCGAGCTGGACAGAG
ACAACCTCAGGCGAAGCCGGCTGAAAAAGGCTCAGACGTTTCCCAGGCTACTGCAGGAAAGCACAGAGAC
CAGCACCCTCTGCCTGTCACTGAACGGCTCGAGAAACCAGCTGGCCATATCTGAGTTCTTTCCCCCAGAC
CCTAGGAAGCTCTTCCTGGAAGGGAAAAATGGCCTTTCTGGTTTCAAAACACAGTCCAAAAGCAGACTGT
GGCCAGAGCAGGAGCCTGGAGTCCAGTTGGATAAGACACCAGGTGCAGGCCGCAACCCACACAGGAGTCC
AGGGGCCTCGGGGCAACTAGAGGCTTCATCTCCTAGCCAGGGATCTGTAGAGTACAAACCTTCACCCAGC
CCTGTAGACGCTGGAGATTTAGACCATGAAGGCCACATACCTAGGAGTGAAGAGAAGATTCCAGAAGAAT
CCAGACAACCTGGAAGCACAGCCACTGAACAGAGCCTGTCCCTGAAGGACCTTCTGTCTAAGCTAGGCCG
GCCCTTCAGGGAGTACGAGCTGTGGGCACTGTGCCTCTCCTGCCTTAGCACTCTGCAGACACACAAGGAG
CACCCAGCTCACCTGTGCCTGGACAACGTGTTGGTGGCCGAGGACGGGACAGTGTTCTTCGGACCGCCCC
CTGCCAATGGCGCCTACAACTCACTCTTCTTGGCTCCCGAGGTGTCAGAAGAGAAGCTGGTGACAGAGAA
GGCCTCTGTGTACTGTGTGGCTGCGGTTCTGTGGACAGCAGCCAAGTTCAGCGTACCCCGAGACCACAAA
CTGGCCCTGCCACGTAGACTCAAAACACTCCTTCTGGACATGGCCAGACGCCATGCCTCAGAACGGCCAT
CTGCAGCCGAGGCCATCAAGGTGTGCAGCAGCTACCTCCTTCAGCGAGGCATGGACAGCAGCAAGATTCT
AGCCCACCTGCGGGCATCCACCTGCAAGGTTCACCCAGAGGAGGAGACCATCGGCCTCCAGAACGCCTTC
TCAGTGGTTGAACTGAAATCCACCACGGCACCTGCTCCTGAGAGTAGTCCCGGCTTCCTGCAGGTCAGCA
ATGATACCAAGCTGGTCGCTGTCCCAGGGCCTGTGCCTGGTCTGCCCCCCTGCTGCAAAGAAGCCTGTGA
GCTGCCAGCAGCCTTCACCTCTGAGGCCACTCACTTTAAGCCCATAGTCCTGGCTCAGGACGCAAGTGTT
ACCAGAGACCAGCTTGCCTTGCCCTCAGAGTCAAATGAGAAACCCAAAGAGGGAAGTGGTCATCTGGACA
GGGAGGGAACAAGAAAGCAGGCAGCCCTGGAGCTTGTTGAGGCCACTGACCTAAAGATGTCCAACCAGCT
GTCACCTGGTCCAGAGCTGCAGGGAGCAACTCCAGACCCTGATGGTGACTCAGGGTCCCCCAGCTCAGCC
ACGGAATGCTCCTGCCCCCATGGCCCTGTTCTGGTCACCCAGCAAAAAGGAACATCAGGGACACCCAGCT
CTCCTGCCTCTTCCCTGCCCCCTGAGCACAGGCCAGATGGTGAGGGACCACTAGACACCACTGTGCTCCC
AGGGCCCACCTCAGCCAGCCAGGGCTCTCGACACCCATGTAAGCCACCCAGGGGTAAGGCTGCAGCGAGT
CCAAGCAGCCCTAGGGGCTCGGATGGTCATCCTGAGAAGCCTCGGCCAGCAGACCGCAAGCTCTGTCCAT
CCAGTGTAGATACCTCATCCCCCCCTAAGATGACAGCCTGCCCCTCGCTGCAGGAAGCCATGCGCCTCAT
CCAGGAGGAATTTGCCTTTGATGGCTACATGGACAACGGGCTAGAGGCACTGATCATGGGGGAGTATATT
TACGCCTTGAAAGACCTCACCTTCGCCACCTTCTGTGGTGCCATATCTGAGAAGTTCTGTGACCTATACT
GGGATGAGCAGCTGCTAAAGAACCTCTTCAAGGTGGTCAATGGGCCAGCCTCACCCTCTGAGAGCACTTC
TGAGGAGCCTGGATCCCAGCCAGAACACTCACCCAGCAGATGCTCACTGTCCAGCAAAAGGCCTTCCCTG
CATGGACTCGGCAAGGAGAAGCCAGCCACGACCTGGGGCAGCGGGGGACCCTGCTCCCCCACAGCACTAT
CAGATATAGACTCCGACACGCTCTCTCAGGGAAACTTTGAGGTTGGATTTCGGTCTCAGAAGTCAATAAA
AGTCACGCGAGAGCAACAACCAGAGGCCGAAGTGGGTGGGCAGCCAGGTCCAAGCCAGGACTCAACCAGC
CATGCCTCGGACACAGTGGCCCGGCTAGCTAGATCTGAGGACGGTGGCCCAGCTGGGTCTCCAGGGGCAT
CTGATTTCCAGAACTGCAGCCCTGGCTGGTCCAGTGCCTTCTATGAGGCTGACTGCTTTGGAGCCGATGT
CTACAACTACGTGAAGGATCTGGAGAGGCAGAAGGCCAACGGGCACACAGAACTGGAAGCCCAGAGTCCA
GAGCTGGAGCAGCAGCTCATGATAGAGAAGAGAAACTATAGGAAGACCCTGAAGTTTTACCAGAAACTCT
TACAGAAGGAAAAACGGAACAAAGGCTCTGAGGTCAGGACCATGTTGTCTAAACTTCGAGGACAGCTGGA
TGAAATGAAGTCCAAGGTGCAGTTCCTGAGCCTGGTCAAGAAGTACCTCCAGGTCATGTATGCAGAACGC
TGGGGGCTGGAGCCCTGTGCTCTGCCGGTGATTGTGAACATCGCAGCAGCCCCCTGTGACACACTGGATT
TCAGCCCCCTGGATGAGTCTTCTTCCCTCATCTTCTACAATGTCAACAAGCACCCAGGCAGCGGCCGACA
GAAGAAGGCTCGCATCCTACAGGCTGGTACACCACTGGGGCTCATGGCCTACCTCTACTCCAGTGATGCT
TTCCTCGAGGGCTACGTGCAGCAGTTTCTCTACACTTTCCGGTACTTTTGCACACCGCACGACTTCCTGC
ACTTCCTCCTGGACCGCATCAGCAGCACTTTATCCAGGGCTCACCAGGATCCTACCTCGACTTTTACCAA
GATCTACAGGCGAAGCCTCTGCGTCCTGCAGGCCTGGGTGGAAGACTGCTACACCGTGGACTTCATAAGG
AACGCGGGGCTGCTGGGACAGCTGGAGGACTTCATCTCTTCCAAGATCCTACCTCTAGATGGCACTGCCG
AGCACCTGCTGGCCCTCTTAGAGGTGGGCACTGAGCGGCGGGCTGACAGTGCCTCTCGAGGTGCAGACCT
GGAGGACCCTAAGGAGGCAGAGGAGGACACCAGACCCTTTAATGCCCTCTGCAAGAGGTTCTCAGAGGAT
GGCATCACCCGGAAGAGCTTCTCTTGGAGACTGCCTCGGGGCAACGGGCTAGTGCTGCCACATCATAAGG
AACGCCAGTACACCATTGCATCCGCCCTGCCTAAGCCCTGCTTCTTCGAAGACTTCTATGGCCCCTATGC
CAAAGCCAGTGAGAAGGGTCCTTACTTCTTGACAGAGTACAGCACCAACCAGCTCTTCACTCAGCTAACA
CTACTGCAGCAGGAATTGTTTCAAAAGTGCCACCCTGTCCACTTCCTAAATTCACGAGCCCTGGGTGTCA
TGGACAAAAGTGCAGCCATTCCCAAAGCCAGCTCTTCTGAGTCTCTTTCTGCCAAAACCTGCAGCCTCTT
CCTACCCAATTACGTCCAGGACAAATATCTGCTACAGCTTTTAAGAAACGCAGATGATGTCAGTACCTGG
GTGGCTGCTGAAATTGTGACCAGTCATACCTCCAAGCTGCAGGTGAACTTGCTGTCCAAATTCCTGCTGA
TTGCAAAATCTTGCTACGAACAGAGGAACTTTGCAACGGCCATGCAAATCCTGGGCGGGCTGGAGCACCT
GGCTGTGAGGCAGTCCCCTGCTTGGAGAATCCTGCCTGCGAAGATTGCTGAGGTCATGGAAGAGCTGAAA
GCTGTGGAGGTATTCCTGAAGAGTGACAGCCTGTGTCTGATGGAAGGAAGGCGCTTCCGGGCCCAGCCTA
CTCTACCCTCCGCCCACCTGCTGGCCATGCACATCCAGCAGCTAGAGACAGGAGGTTTCACCATGACTAA
TGGAGCCCATAGATGGAGCAAACTGAGAAACATCGCCAAGGTGGCAAGCCAGGTGCACGCGTTCCAGGAG
AACCCGTACACATTCAGCCCGGACCCCAAGCTACAGGCCCACCTCAAGCAGAGGATCGCCCGCTTTAGCG
GCGCTGATGTCTCCATTTTAGCAGCTGATAACAGGGCCAACTTCCACCAGATCCCAGGAGAGAAACACTC
ACGGAAGATCCAAGACAAGCTGAGGAGAATGAAGGCCACATTCCAG

ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216490 representing NM_177261
Red=Cloning site Green=Tags(s)

MQAMDPASRGFYEEDGKDLDFYDFEPLPTLPEDEENVSLADILSLRDRGLSEQEAWAVCLECSLSMRSVA
HAAIFQTLCITPDTLAFNTSGNVCFMEQLSDDPEGAFVPPEFDLTGNTFEAHIYSLGATLKAALEYVPEP
ELEPKLSTDLEGLLSQMQAEDPRERPDLASIIALCEEKMQPVSSCRLCRSLSAIGRRVLSIESFGAFQEL
SENTWRGRPAPRNVGPKKMPGDLSTDPEALFPSKGLLQPPASRDAEQEAGQRPRAPSPKPLLSAPVRNGE
NPGQEGLADLVLDARCPLGELDRDNLRRSRLKKAQTFPRLLQESTETSTLCLSLNGSRNQLAISEFFPPD
PRKLFLEGKNGLSGFKTQSKSRLWPEQEPGVQLDKTPGAGRNPHRSPGASGQLEASSPSQGSVEYKPSPS
PVDAGDLDHEGHIPRSEEKIPEESRQPGSTATEQSLSLKDLLSKLGRPFREYELWALCLSCLSTLQTHKE
HPAHLCLDNVLVAEDGTVFFGPPPANGAYNSLFLAPEVSEEKLVTEKASVYCVAAVLWTAAKFSVPRDHK
LALPRRLKTLLLDMARRHASERPSAAEAIKVCSSYLLQRGMDSSKILAHLRASTCKVHPEEETIGLQNAF
SVVELKSTTAPAPESSPGFLQVSNDTKLVAVPGPVPGLPPCCKEACELPAAFTSEATHFKPIVLAQDASV
TRDQLALPSESNEKPKEGSGHLDREGTRKQAALELVEATDLKMSNQLSPGPELQGATPDPDGDSGSPSSA
TECSCPHGPVLVTQQKGTSGTPSSPASSLPPEHRPDGEGPLDTTVLPGPTSASQGSRHPCKPPRGKAAAS
PSSPRGSDGHPEKPRPADRKLCPSSVDTSSPPKMTACPSLQEAMRLIQEEFAFDGYMDNGLEALIMGEYI
YALKDLTFATFCGAISEKFCDLYWDEQLLKNLFKVVNGPASPSESTSEEPGSQPEHSPSRCSLSSKRPSL
HGLGKEKPATTWGSGGPCSPTALSDIDSDTLSQGNFEVGFRSQKSIKVTREQQPEAEVGGQPGPSQDSTS
HASDTVARLARSEDGGPAGSPGASDFQNCSPGWSSAFYEADCFGADVYNYVKDLERQKANGHTELEAQSP
ELEQQLMIEKRNYRKTLKFYQKLLQKEKRNKGSEVRTMLSKLRGQLDEMKSKVQFLSLVKKYLQVMYAER
WGLEPCALPVIVNIAAAPCDTLDFSPLDESSSLIFYNVNKHPGSGRQKKARILQAGTPLGLMAYLYSSDA
FLEGYVQQFLYTFRYFCTPHDFLHFLLDRISSTLSRAHQDPTSTFTKIYRRSLCVLQAWVEDCYTVDFIR
NAGLLGQLEDFISSKILPLDGTAEHLLALLEVGTERRADSASRGADLEDPKEAEEDTRPFNALCKRFSED
GITRKSFSWRLPRGNGLVLPHHKERQYTIASALPKPCFFEDFYGPYAKASEKGPYFLTEYSTNQLFTQLT
LLQQELFQKCHPVHFLNSRALGVMDKSAAIPKASSSESLSAKTCSLFLPNYVQDKYLLQLLRNADDVSTW
VAAEIVTSHTSKLQVNLLSKFLLIAKSCYEQRNFATAMQILGGLEHLAVRQSPAWRILPAKIAEVMEELK
AVEVFLKSDSLCLMEGRRFRAQPTLPSAHLLAMHIQQLETGGFTMTNGAHRWSKLRNIAKVASQVHAFQE
NPYTFSPDPKLQAHLKQRIARFSGADVSILAADNRANFHQIPGEKHSRKIQDKLRRMKATFQ

TRPLE - GFP Tag - V
Restriction Sites SgfI-NotI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177261
ORF Size 5226 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177261.4, NP_796235.4
RefSeq Size 7320 bp
RefSeq ORF 5229 bp
Locus ID 76484
UniProt ID Q0KK55
Cytogenetics 7 F4
Summary RAS-Guanine nucleotide exchange factor (GEF) that controls the negative regulation of neuronal dendrite growth by mediating a signaling pathway linking RAS and MAP2 (PubMed:17984326, PubMed:21385318). May be involved in cellular senescence (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177261" in other vectors (2)
SKU Description Size Price
MC224892 Kndc1 (untagged) - Mouse kinase non-catalytic C-lobe domain (KIND) containing 1 (Kndc1), (10ug) 10 ug
$1,611.00
MR216490 Kndc1 (Myc-DDK-tagged) - Mouse kinase non-catalytic C-lobe domain (KIND) containing 1 (Kndc1) 10 ug
$1,465.00

file-alt Citations

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