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D630045J12Rik (NM_194061) Mouse Tagged ORF Clone

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SKU
MG216488
D630045J12Rik (tGFP-tagged) - Mouse RIKEN cDNA D630045J12 gene (D630045J12Rik), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,830.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "D630045J12Rik or NM_194061"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D630045J12Rik
Synonyms Kiaa1549; mKIAA1549
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216488 representing NM_194061
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGGAGCCCCGCGCCAGGGTTGCACTGGTGCCGGAGCGGTGCGGCCGCGGGCCTTCCTCCCGCC
ACCGCCGCCCGGGGCTGCTGCTCCCCGGCCTCTGGCTGCTGCTGCTCGCCGGGCCGGCCTCGTGCGCCCC
AGATGACCTCTCCCTGGCTCAGCACAGCCATCCTGTACGCCCCTCGGACTTCCTGCCAGAGAGAAGCATC
CTGCACAGTGCTGCACAAGTGACCTTATCAGAGACTGTTCCCAGGTCCCAACCAAGCATCTCTGCCCTTG
TCCTCTCCTCTCCGTCAGCGACAGCTTTTGATACAGCCTTTTTAAGCCAGAGACAACAGACTCAAAGTAC
AGCGGAGCCCAGTTTCTTTGAGGCAAATTACGGATCCGTGACGAGTAATGAGGTGGCCCTCGATGATGAG
GAGATGGATAACTTTTTGCCAGATGCTCATTGGACCTCTTCAAGGGGGGTTTCCCCAATGCGGTATATCA
CACCGAGTCCACCAGAGCCACCCCAGGAAATGCTGGAGCCTGGCACGACCCCATCACTTCCCACCATTTC
TTTGCCGGATGAAGTGCTGTCCGGATGTCAGAACACAGTGCAACAGGCAACTGTATATGTGGAGCCGTCC
ACGTACTTCGGCACTTCTTGGTCAGCTTTTCTCACGTCTGAGGGCATCATTCCAACTCCTAGTAGGAATT
CGGTGCTTCATCCTATAGAAATTCACAGCCAGTTATCAAGCAAGGCTTTGCCAGAGACAGTAGCTTCTGT
AACAGAGGGCGCAGAAAACCTCCTTTTTAGCTCCCGGATTTCAGTGTCTCAGCCATCAGGCAATGGCATG
ACTCAACAGCCATCTGTCCCCCTGTGGGAGGTCTCACAGCCTCTAGTGGGTGTCCTGGCCACAAGCTCGG
ACAGATACCCTAATGAGACCACTGCTTGGATCGAACACCCAGAGGAAGCCATCGCTCTAAGAGCACACCC
TGGCATAACCACGTCACCCACAGACCCCACTTTTAGCAGTCAGCCTTCTGCTCTATTTTCAACCCCACTT
TCATCTGTGTCTTTTGCTACCCAATTACCTGGTGTTTCGGAGGACTCCTTCCTCTCTAGTGAGGCCAGGG
GAGCCTTAGAGTCATGGCATAGCTCCCCAGTCCCCAGTTTTACTGACCATCCTTATGTCCTGAGCCCCGA
GTCTTCCCTGAGACCACACACAGGGTGTGTGTCTTGCGTTGTCTCATCATTTCAACAAGAACTGGCCAGG
AGCCTAACAGAGAAGGACATGGGGTCAGGCGACAGGCTTGAGACTTTGTCTACTGCCTCGGTAGAGGCAA
GTCATGTCTCTCCATTGAGCAGTGTGGGCACAGACCTCTCTGAACTTGAAGAGCCTCAAGAATTTAATAC
GCTTTTCCCTTCCAGACCTGTGTTTTCCTTTTCTTCCAGACCTGTGGGGCTGTGGAAAGCCAGCATGGAT
GTGTCACCCGAGGTGGATGTGAGTGGCATTGCCATCACTCAGGTGTACCCTTCCCATGGCCGCCTCTCCA
CACCAAGCTCACTGGATTCGACTTTTGGCTTCTCTGTCACCAGTGATCTAGTGATGTCATCCAGTATGAT
CCATCTGTTGTCCTCTGTCATCCCCAGCACCCATTTTGACTCATCTTTCTCCTTGACTGCAAATCAAAAC
AGTCCATCCTTTCCAGCTGGAAAGCCAAGCCTTCTCACCTCTCCCAGTCTGGTCCCCTCAGCACAGTCTA
GCGCTTTCTCTCACGGAGCACCTACCAGCTCTCTGGAACTCCAGTCTGGAAGTCGTTTGGATTTCACATC
CGGCTTTTACTCCACTCCGCCCCTGGACTTCAGCACTCCAGCCCCTTCACGGTCAGACGAGCTTGCTTTC
CCATCTTTGATGTCAAGCGATCCATCAACCTTCTTTTCTCAAACTTTTTCCACCATGGCTGAGACATTTT
CACTGTCCAACTCTATGAATTTGCAATCACCTCAGCTTTCTGTTCTTAATCCCACAAGTCTAGAGCCGTC
TCAGCCACAGTCAAGTGCAGACCTTCTTTTGAACACAGTCACTGTTCTTCCTAGTCCCCCCGAGAGGCCC
CCACTTTCAAGCTCCCCCTCTGACTCTCTCGAGTTCACCGAAGTATCCAGAGTTTCACTGAGAGAATCCC
ATGTTCATCTTACCTCAGCTTTCTCTGAAACTACCTCTGCCTTTGAATTTTCACCCATTCCCCATGAGTC
CACTATCAGCACACTGGTGCCCTCCAGCTCTGAGCCCTCTCTTGGCATCTACGCAGCTGGGACTCACCTC
ACATCACTGCCAACAACTGTTTTCCATTTGACACCCATTTTAACCGAGTCATCTCCATTCTCGACTCTGA
CACCTTCCGATGGCAGTGTGCGTGTGACAGATCATCACACACCAGTCTTACCCACACCCTCCAGTGTCAT
TCCAAGCAGCACAGAGTCGATCTCTGACCCGTACCTGTCAGCCTCCTCATCACTTGTTTCTGAAGTAAGC
CCTTCACCTCCGCCCACAAAGCCAGTCATGGGCTCATCATTGACATCCACAGATTTCCCGCCCAGTACTT
CCCCAGAACTAAGTACATCCCTGGAACTGAGCATGCCCAGTGCCAGCACTGCCCCTGGTGATACTGTGGA
CTCTGCTCTGAACAGCGAGCCTATGAGTCAGAACCCTCAGGGGAACAGTGTACCCCCTCCCCAACCCTCC
CTCGGTCCTGCTACCACCTCCACGCTTGAAGCCACAGTTGGTACACCAGCACTGGCCACAGCCAAGCCGC
CATATGTGTGTGATATTACGGTTCCCGATGTCTATTTGATCACAACTGTGCTGGCCAGACGAGCTGTGCA
GGAGTACATCATTACATCAATTAAAGAAGTGCTGAGAATCCACTTCAACCGTGCGGTGGAGCTAAAGGTT
TATGAACTGTTCACAGACTTCACATTTCTGGTAACGTCTGGTCCTTTTGTTTACACGGCGATATCAGTCA
TCAACGTCCTCATCAATAGCAAACTTGTGCGTGGCCAGACGCCTTTAATCCTGTCTGTGAAGCCATCTTT
TCTCGTGCCAGATCCCAGGTTTCAAGTTCAAACAGTACTGCAGTTCGTGCCTCCGAGTGTCGATACTGGC
TTCTGCAACTTCACTCAGAGCATTGAGAAAGGCCTGGTGACAGCTCTCTTTGAAGTGAGGAAACACCAGG
GAACATATAACCTCACAGTGCAGATTGTGAATGTCACCATTGCTTCTTCGAGGGTGGCTCCCCGGAGGGG
CCCAGTGAATATTATCTTTGCTGTTAAAGGCTCGCAAGGTTTTCTGAATGGGTCGGAGGTGAGCGATCTG
CTGAGGAACCTGACGGTGGTGGAGTTCAGCTTCTACCTGGGGTACCCGGTGCTACAGATCGCAGAGCCCT
TCCAATACCCGCAGCTCAATTTATCTCAGTTACTGAAGTCCTCTTGGGTCAGAACAGTCCTCCTGGGTGT
AGTGGAAAAACAGCTCCACAATGAAGTGTTTCCAGCCGAGATGGAGCGCAAACTGGCTCAGCTGCTCAGC
GAGGTGCCCACCCGAAGACGCGTGTGGCGGAGAGCCACTGTAGCTGCTGGGAACAGCGTGGTACAGGTGG
TGAACGTGTCCAGGCTGGAGGGAGATGACAACCCGGTGCAGCTCATCTACTTTGTGGAGAATCAAGATGG
AGAGAGACTCAGTGCAGTCAAGTCTTCAGATCTGATCAACAAGGTAGACCTCCAGAGGGCAGCCATCATC
CTGGGGTACCGGATTCAAGGCGTCATTGCCCAGCCTGTGGACAGGGTGAAGAGGCCATCCCCAGAGTCAC
AGAGCAACAACCTCTGGGTCGTGGTTGGCGTGGTCATTCCCGTGCTGGTGGTCACCGTAATTGTTGTTAT
CCTCTACTGGAAGTTGTGCCGCACGGACAAGCTAGACTTCCAGCCAGACACGGTGGCCAACATACAGCAG
CGGCAGAAGCTGCAGATCCCCAGCGTGAAGGGCTTCGATTTTGCTAAGCAGCATCTGGGCCAGCACAATA
AAGACGACATCCTGATTATTCATGAGCCAGCGCCACTGCCAGGCCCTGTGAAGGACCACACCACACCCTC
AGAAAACGGAGATGTGCCGAGCCCCAAGTCAAAGCTCCCTTCCAAGAACATACGGCTCAGAGGGAGAGTT
TCCCCCTCAGATGCTGACTCAACAGTCAGCGAGGAGTCCAGCGAGAGGGACGCGGGAGAGAAGGCGCCGG
CTGCTGCCCCTGAGAACAAGGCTCTCAGAGCTCCGCAGAGTGGCGCCCCCCTGCCCAGTTCAGGCAATGA
GCAGCATTCCTCAGCCTCCATCTTTGAGCACGTGGACAGGGTTTCGAGGACCTCAGAGGCCAGCCGCCGG
GTCCCCAGTAAGATCCAGCTGATTGCCATGCAGCCCATCCCGGCCCCTCCAGCGCAGCACCCTGTCCTGG
CCGACCGAGTGGCAGAGACCAACAAAATCAACAAGGAGATCCAGTCAGCACTGCGCCACAAGTCGGAGAT
TGAGCATCACCGCAACAAGATTCGCCTTCGTGCCAAACGCCGAGGCCACTACGAGTTCCCGGTGGTGGAT
GACCTGTCCTCTGGTGACACCAAGGAGCGACACCGAGTATACCGCAGGGCACAAATGCAGATTGACAAGA
TCCTGGACCCCGCAGCCAGTGTGCCCTCTGTGTTCATAGAGCCCAGGAAGAGCTCACGGATGAAACGTTC
TCCTAAGCCACGCCGGAAACACCAGGTCAACGGCTGTCCCGCTGATGCGGAGAAGGACCGACTCATCACC
ACAGACAGTGACGGCACATACAAGCGGCCTCCCGGTGTGCACAACTCTGCCTACATCGGATGCCCGTCTG
ATCCTGACCTCCCAGCTGATGTCCAGACACCCTCCTCTACAGAGCTGGGCAGGTATCCCGGCCTGCCCTT
CTCAGCCTCTCAGTACATCCCGCCTCAGCCGTCCATCGAGGAGGCACGCCAGACCATGCACTCCCTCCTG
GATGACGCCTTTGCCCTCGTGGCTCCCAGCAGCCAGCCCACCAATGCTATGGGAGCGGGTACTGGTGTGC
CTGCCAGCCTACCTGTGAATAGCACCCCGTCCAGGGAAGAGAGGCGAGCCACGCAGTGGGGGTCCTTCTA
CAGTCCAGCTCAGACGGCCAACAACCCTTGCAGCAGATACGAGGACTATGGGATGACTCCCCCATCGGGC
CCATTGCCAAGCAGACCTAGCTTTGGCCCAGGGTTGCTGCCGTCTTCAGAGTTGGTTCCCCCTGAACCCC
CACAGCCACAGTCATCAACTGATGCTCCATATGCTGCCCGAGGGATCTACTCAGAGGAGATGCCATCAGT
GGCCCGGCCTCGGCCTGTTGGGGGCACCACAGGCTCCCAGATCCAGCATCTGACACAGGTGGGGATTGCA
AGCAGAATCGGGGCCCAGCCAGTGGAGATTCCTGCCGGCAGAGGCAGCCAGTACGGGGGACCAGGCTGGC
CTGTGTACGGGGAGGAGGAGGCAGGGCGAAGAGAAGCCACCCACATGCTCGGACATCAAGAGTATTCTTC
TTCACCGCTGTTTCAGGTGCCAAGGACTTCGGGCAGGGAGCCCTCAGCCCCTCCCGGGAACCTCGCCCAC
CGAGGGCTGCAGGGCCCTGGGCTGGGTTACCCCACCAGCTCCACAGAGGACCTCCAGCCCGGCCACTCCT
CCGCCTCCCTCATCAAAGCGATCCGCGAGGAGCTCCTGCGGCTCTCTCAGAAACAGGGCTCAGTACAGAA
CTTCCACAGC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216488 representing NM_194061
Red=Cloning site Green=Tags(s)

MEREPRARVALVPERCGRGPSSRHRRPGLLLPGLWLLLLAGPASCAPDDLSLAQHSHPVRPSDFLPERSI
LHSAAQVTLSETVPRSQPSISALVLSSPSATAFDTAFLSQRQQTQSTAEPSFFEANYGSVTSNEVALDDE
EMDNFLPDAHWTSSRGVSPMRYITPSPPEPPQEMLEPGTTPSLPTISLPDEVLSGCQNTVQQATVYVEPS
TYFGTSWSAFLTSEGIIPTPSRNSVLHPIEIHSQLSSKALPETVASVTEGAENLLFSSRISVSQPSGNGM
TQQPSVPLWEVSQPLVGVLATSSDRYPNETTAWIEHPEEAIALRAHPGITTSPTDPTFSSQPSALFSTPL
SSVSFATQLPGVSEDSFLSSEARGALESWHSSPVPSFTDHPYVLSPESSLRPHTGCVSCVVSSFQQELAR
SLTEKDMGSGDRLETLSTASVEASHVSPLSSVGTDLSELEEPQEFNTLFPSRPVFSFSSRPVGLWKASMD
VSPEVDVSGIAITQVYPSHGRLSTPSSLDSTFGFSVTSDLVMSSSMIHLLSSVIPSTHFDSSFSLTANQN
SPSFPAGKPSLLTSPSLVPSAQSSAFSHGAPTSSLELQSGSRLDFTSGFYSTPPLDFSTPAPSRSDELAF
PSLMSSDPSTFFSQTFSTMAETFSLSNSMNLQSPQLSVLNPTSLEPSQPQSSADLLLNTVTVLPSPPERP
PLSSSPSDSLEFTEVSRVSLRESHVHLTSAFSETTSAFEFSPIPHESTISTLVPSSSEPSLGIYAAGTHL
TSLPTTVFHLTPILTESSPFSTLTPSDGSVRVTDHHTPVLPTPSSVIPSSTESISDPYLSASSSLVSEVS
PSPPPTKPVMGSSLTSTDFPPSTSPELSTSLELSMPSASTAPGDTVDSALNSEPMSQNPQGNSVPPPQPS
LGPATTSTLEATVGTPALATAKPPYVCDITVPDVYLITTVLARRAVQEYIITSIKEVLRIHFNRAVELKV
YELFTDFTFLVTSGPFVYTAISVINVLINSKLVRGQTPLILSVKPSFLVPDPRFQVQTVLQFVPPSVDTG
FCNFTQSIEKGLVTALFEVRKHQGTYNLTVQIVNVTIASSRVAPRRGPVNIIFAVKGSQGFLNGSEVSDL
LRNLTVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVVEKQLHNEVFPAEMERKLAQLLS
EVPTRRRVWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVENQDGERLSAVKSSDLINKVDLQRAAII
LGYRIQGVIAQPVDRVKRPSPESQSNNLWVVVGVVIPVLVVTVIVVILYWKLCRTDKLDFQPDTVANIQQ
RQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPVKDHTTPSENGDVPSPKSKLPSKNIRLRGRV
SPSDADSTVSEESSERDAGEKAPAAAPENKALRAPQSGAPLPSSGNEQHSSASIFEHVDRVSRTSEASRR
VPSKIQLIAMQPIPAPPAQHPVLADRVAETNKINKEIQSALRHKSEIEHHRNKIRLRAKRRGHYEFPVVD
DLSSGDTKERHRVYRRAQMQIDKILDPAASVPSVFIEPRKSSRMKRSPKPRRKHQVNGCPADAEKDRLIT
TDSDGTYKRPPGVHNSAYIGCPSDPDLPADVQTPSSTELGRYPGLPFSASQYIPPQPSIEEARQTMHSLL
DDAFALVAPSSQPTNAMGAGTGVPASLPVNSTPSREERRATQWGSFYSPAQTANNPCSRYEDYGMTPPSG
PLPSRPSFGPGLLPSSELVPPEPPQPQSSTDAPYAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIA
SRIGAQPVEIPAGRGSQYGGPGWPVYGEEEAGRREATHMLGHQEYSSSPLFQVPRTSGREPSAPPGNLAH
RGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQGSVQNFHS

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_194061
ORF Size 5820 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194061.3
RefSeq Size 10986 bp
RefSeq ORF 5823 bp
Locus ID 330286
UniProt ID Q68FD9
Cytogenetics 6 B1
Summary May play a role in photoreceptor function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_194061" in other vectors (2)
SKU Description Size Price
MC225067 D630045J12Rik (untagged) - Mouse RIKEN cDNA D630045J12 gene (D630045J12Rik), (10ug) 10 ug
$1,794.00
MR216488 D630045J12Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630045J12 gene (D630045J12Rik) 10 ug
$1,630.00

file-alt Citations

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