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Chrdl1 (NM_001114385) Mouse Tagged ORF Clone

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SKU
MG216466
Chrdl1 (tGFP-tagged) - Mouse chordin-like 1 (Chrdl1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Chrdl1 or NM_001114385"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chrdl1
Synonyms CHL; CHL1; Nrln1; VOPT
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216466 representing NM_001114385
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGCATGAAATACATCATTTCCTTATTTTTCATCTTTGTTTTCCTAGAAGGAAGCAAAACAGAAC
AAGTAAAACACTCAGACACATATTGCGTGTTTCAAGACAAGAAGTATAGAGTGGGTGAGAAATGGCATCC
CTACCTGGAACCGTATGGACTGGTTTACTGTGTGAACTGCATCTGCTCTGAGAATGGGAATGTGCTTTGC
AGCCGAGTCAGATGTCCAAGTCTTCATTGCCTTTCACCCGTGCATATTCCTCATCTCTGTTGCCCCCGCT
GCCCAGACTCCTTACCACCAGTGAACAATAAGGTGACCAGCAAGTCATGCGAATACAATGGAACCACTTA
CCAACATGGAGAACTGTTCATAGCTGAAGGGCTCTTTCAGAACCGGCAACCCAATCAGTGCAGTCAGTGT
AGCTGCTCGGAGGGGAATGTATACTGTGGTCTCAAGACTTGCCCCAAACTGACCTGTGCATTCCCAGTCT
CTGTTCCAGATTCTTGCTGCCGAGTATGCAGAGGGGATGCAGAATTATCGTGGGAACATGCGGATGGTGA
TATCTTCCGGCAACCTGCCAACAGAGAAGCAAGACATTCTTACCTCCGTTCCCCCTACGATCCTCCACCA
AACAGACAAGCTGGAGGTCTTCCCCGCTTTCCTGGGAGCAGAAGTCACCGGGGAGCTGTTATAGATTCCC
AGCAAGCATCCGGGACCATCGTGCAGATTGTCATCAATAACAAGCACAAACATGGACAAGTGTGTGTTTC
CAATGGAAAGACCTACTCTCATGGAGAGTCCTGGCACCCAAATCTACGAGCATTTGGCATTGTGGAATGT
GTACTATGCACTTGTAATGTCACCAAGCAAGAATGTAAGAAAATCCACTGCCCCAATCGATACCCCTGCA
AGTATCCTCAAAAAATAGATGGAAAGTGCTGCAAGGTGTGCCCAGAAGAACCTCCAAGCCAAAACTTTGA
CAGCAAAGGTTCCTTTTGTGGAGAAGAAACCATGCCTGTATATGAGTCTGTGTTCATGGAGGATGGAGAG
ACAACCAGAAAAGTAGCACTGGAGACCGAGAGACCACCTCAAGTAGAGGTCCACGTTTGGACTATTCAAA
AGGGCATTCTCCAGCACTTCCACATTGAGAAGATTTCCAAGAGGATGTTTGGGGAGCTCCATCATTTCAA
GCTAGTTACTCGGACCACCTTGAACCAGTGGAAGCTCTTCACTGAAGGAGAAGCTCAGCTCAGCCAGATG
TGCTCAAGTCAGGTGTGCAGAACAGAGCTGGAAGATTTAGTCCAGGTTTTGTACCTGGGGAGACCTGAAA
AGGACCACTGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216466 representing NM_001114385
Red=Cloning site Green=Tags(s)

MDGMKYIISLFFIFVFLEGSKTEQVKHSDTYCVFQDKKYRVGEKWHPYLEPYGLVYCVNCICSENGNVLC
SRVRCPSLHCLSPVHIPHLCCPRCPDSLPPVNNKVTSKSCEYNGTTYQHGELFIAEGLFQNRQPNQCSQC
SCSEGNVYCGLKTCPKLTCAFPVSVPDSCCRVCRGDAELSWEHADGDIFRQPANREARHSYLRSPYDPPP
NRQAGGLPRFPGSRSHRGAVIDSQQASGTIVQIVINNKHKHGQVCVSNGKTYSHGESWHPNLRAFGIVEC
VLCTCNVTKQECKKIHCPNRYPCKYPQKIDGKCCKVCPEEPPSQNFDSKGSFCGEETMPVYESVFMEDGE
TTRKVALETERPPQVEVHVWTIQKGILQHFHIEKISKRMFGELHHFKLVTRTTLNQWKLFTEGEAQLSQM
CSSQVCRTELEDLVQVLYLGRPEKDHC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001114385
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001114385.1, NP_001107857.1
RefSeq Size 4060 bp
RefSeq ORF 1344 bp
Locus ID 83453
UniProt ID Q920C1
Cytogenetics X F2
Summary Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001114385" in other vectors (4)
SKU Description Size Price
MC216023 Chrdl1 (untagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 1, (10ug) 10 ug
$503.00
MR216466 Chrdl1 (Myc-DDK-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 1 10 ug
$289.00 $457.00
MR216466L3 Lenti ORF clone of Chrdl1 (Myc-DDK-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 1 10 ug
$757.00
MR216466L4 Lenti ORF clone of Chrdl1 (mGFP-tagged) - Mouse chordin-like 1 (Chrdl1), transcript variant 1 10 ug
$757.00

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