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Acer2 (NM_139306) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216452
Acer2 (tGFP-tagged) - Mouse alkaline ceramidase 2 (Acer2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Acer2 or NM_139306"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Acer2
Synonyms 2410116I05Rik; Asah3l; CRG-L1; maCER2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216452 representing NM_139306
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCCCCGCACTGGTGGGACCACCTGCGGGCTGGCAGTTCGGAGGTGGATTGGTGCGAGGACAACT
ACACTATCGTGCCTGCCATTGCCGAGTTCTACAACACGATCAGCAACGTCTTGTTTTTCATTTTACCTCC
CATCTGCATGTGCTTGTTCCGCCAGTACGCAACGTGCTTCAACAGCGGCATCTACTTAATATGGACGCTC
CTAGTTGTAGTGGGGATTGGATCTGTCTACTTCCATGCAACGCTGAGTTTCCTGGGTCAGATGCTTGATG
AACTTGCCATTCTGTGGGTTCTGATGTGTGCTTTGGCCATGTGGTTTCCCAGGAGGTATTTACCAAAGAT
CTTTCGGAATGACAGGGGCAGGTTCAAGGCAGTGGTGTGTGTCCTGTCTGCAATTACAACGTGCTTGGCG
TTTATCAAGCCCGCCATCAACAATATTTCCCTGATGATTCTGGGACTTCCATGCACTGCGCTGCTTGTTG
CAGAGCTGAAGAGGTGTGACAATGTGCGTGTGTTTAAGCTGGGCCTCTTCTCTGGCCTCTGGTGGACTCT
GGCTCTCTTCTGCTGGATCAGCGACCAAGCCTTCTGTGAGCTGCTCTCCTCCTTTCACTTCCCCTACCTG
CACTGTGTGTGGCATATTCTCATCTGCCTTGCTTCGTACCTGGGCTGTGTGTGCTTCGCCTACTTTGATG
CTGCCTCAGAGATACCTGAGCAAGGTCCAGTCATCAGATTCTGGCCCAGCGAGAAATGGGCTTTTATTGG
TGTCCCTTATGTGTCCCTTCTGTGTGCCCACAAGAAGTCGCCAGTCAAGATCACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216452 representing NM_139306
Red=Cloning site Green=Tags(s)

MGAPHWWDHLRAGSSEVDWCEDNYTIVPAIAEFYNTISNVLFFILPPICMCLFRQYATCFNSGIYLIWTL
LVVVGIGSVYFHATLSFLGQMLDELAILWVLMCALAMWFPRRYLPKIFRNDRGRFKAVVCVLSAITTCLA
FIKPAINNISLMILGLPCTALLVAELKRCDNVRVFKLGLFSGLWWTLALFCWISDQAFCELLSSFHFPYL
HCVWHILICLASYLGCVCFAYFDAASEIPEQGPVIRFWPSEKWAFIGVPYVSLLCAHKKSPVKIT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139306
ORF Size 825 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139306.3, NP_647467.1
RefSeq Size 4204 bp
RefSeq ORF 828 bp
Locus ID 230379
UniProt ID Q8VD53
Cytogenetics 4 C4
Summary Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:29401619). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:29401619). Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides (By similarity) (PubMed:29401619). Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates. Also hydrolyzes dihydroceramides to produce dihydrosphingosine (By similarity). It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells (PubMed:29401619). Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate. As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage. Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139306" in other vectors (4)
SKU Description Size Price
MC212879 Acer2 (untagged) - Mouse alkaline ceramidase 2 (Acer2), (10ug) 10 ug
$330.00
MR216452 Acer2 (Myc-DDK-tagged) - Mouse alkaline ceramidase 2 (Acer2) 10 ug
$289.00 $300.00
MR216452L3 Lenti ORF clone of Acer2 (Myc-DDK-tagged) - Mouse alkaline ceramidase 2 (Acer2) 10 ug
$600.00
MR216452L4 Lenti ORF clone of Acer2 (mGFP-tagged) - Mouse alkaline ceramidase 2 (Acer2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.