Fbxo44 (NM_173401) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216410
Fbxo44 (tGFP-tagged) - Mouse F-box protein 44 (Fbxo44) transcript variant 1, (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxo44
Synonyms 5730411K09; AV001623; FBG3; Fbx6a; FBX30; Fbxo6a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216410 representing NM_173401
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTAGGCAACATCAACGAGCTGCCAGAGAACATCCTGCTGGAACTGTTCATCCACATCCCAGCTC
GCCAACTGCTGCTGCGCTGCCGACCGGTCTGCAGCCTCTGGCGAGACCTCATTGACCTGGTTACACTCTG
GAAGCGCAAGTGCCTTCAAGAGGGCTTCATCACCGAGGACTGGGACCAGCCTGTGGCTGACTGGAAGATC
TTCTACTTCCTGCGCAGCCTCCAGAGGAACCTCCTTCACAACCCTTGTGCTGAAGAGGGATTTGAGTTCT
GGAGCCTGGACGTGAACGGAGGCGATGAATGGAAGGTAGAGGATCTCTCTAAAGACCAGCGAAAGGAATT
CCCCAATGACCAGGTCAAGAAATACTTCGTGACTTCCTATTACACCTGCCTCAAGTCCCAGGTGGTGGAC
CTCAAGGCTGAAGGGTATTGGGAGGAGCTGATGGACACCACCCGGCCGGACATCGAGGTCAAGGACTGGT
TTGCAGCCAGGCCAGACTGCGGGTCCAAGTACCAGCTGTGCGTCCAACTCCTGTCGTCAGCCCACGCACC
ACTGGGAACCTTCCAGCCGGACCCGGTGATGATTCAGCAGAAAAGCGATGCCAAGTGGAGGGAGGTTTCA
CACACATTCTCCAACTATCCTCCCGGCGTCCGCTACATCTGGTTTCAGCACGGAGGCGTGGACACTCACT
ACTGGGCCGGCTGGTACGGCCCGAGAGTCACAAACAGCAGCATCACCATCGGGCCCCCGCTGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216410 representing NM_173401
Red=Cloning site Green=Tags(s)

MAVGNINELPENILLELFIHIPARQLLLRCRPVCSLWRDLIDLVTLWKRKCLQEGFITEDWDQPVADWKI
FYFLRSLQRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLSKDQRKEFPNDQVKKYFVTSYYTCLKSQVVD
LKAEGYWEELMDTTRPDIEVKDWFAARPDCGSKYQLCVQLLSSAHAPLGTFQPDPVMIQQKSDAKWREVS
HTFSNYPPGVRYIWFQHGGVDTHYWAGWYGPRVTNSSITIGPPLP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173401
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173401.5, NP_775577.2
RefSeq Size 1813 bp
RefSeq ORF 768 bp
Locus ID 230903
UniProt ID Q8BK26
Cytogenetics 4 E2
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173401" in other vectors (4)
SKU Description Size Price
MC212907 Fbxo44 (untagged) - Mouse F-box protein 44 (Fbxo44), transcript variant 1, (10ug) 10 ug
$330.00
MR216410 Fbxo44 (Myc-DDK-tagged) - Mouse F-box protein 44 (Fbxo44), transcript variant 1 10 ug
$289.00 $300.00
MR216410L3 Lenti ORF clone of Fbxo44 (Myc-DDK-tagged) - Mouse F-box protein 44 (Fbxo44), transcript variant 1 10 ug
$600.00
MR216410L4 Lenti ORF clone of Fbxo44 (mGFP-tagged) - Mouse F-box protein 44 (Fbxo44), transcript variant 1 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.