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Atp13a1 (NM_133224) Mouse Tagged ORF Clone

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SKU
MG216408
Atp13a1 (tGFP-tagged) - Mouse ATPase type 13A1 (Atp13a1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,245.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp13a1
Synonyms Atp13a; catp; Cgi152
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216408 representing NM_133224
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGGTGGGCAACGCGGTTCCTTGCGGAGCCCGGCCTGGCGGGGCCCGGGACAACGGGTCTCCTC
AGCCTGGGTCGCGATTGCGGCCGGGCCTAGCTGCCGGGCCAGCACTCATAGCGAACGGCGACGAACTGGT
GGCCGCCGTGTGGCCGTACCGGCGGCTGGCGCTTCTGCGGCGCCTCACCGTGCTGCCGTTTGCCGGGCTG
CTGTACCCAGCCTGGCTGGGCGCCGCCGCCAGCGGTTGCTGGGGATGGGGCAGCAGCTGGACCCAGATAC
CCGAGGCCGCGCTGCTGGCGCTCGCCACCATCTGCCTCGCGCACGCACTCACTGTCCTGTCGGGGCATTG
GTCTGTGCACGCGCACTGCGCGCTCACCTGCACCCCGGAGTATGATCCCAACAAAGTAACCTTTGTGAAG
GTGGTGCCAACCCCCAACAATGGCTCCACTGAGCTTGTGGCCCTGCACCGTGACAAGGGTGAAGATGGGC
TGGAGGTGCTGTCATTTGAATTCCAGAAGATCAAATATTCCTATGATGCCCTGGAGAAAAAGCAGTTCCT
TCCGGTGGCCTTCCCAGTGGGGAATGCCTTCTCTTACTATCAAAGCAACAGAGGCTTCCAGGAAGACTCA
GAGATCCGAGCTGCAGAGAAGAAGTTTGGAAGCAACAAGGCTGAGATGGTAGTGCCCGACTTCTCTGAGC
TTTTCAAGGAGAGAGCCACAGCCCCTTTCTTCGTGTTTCAGGTATTCTGCGTTGGGCTCTGGTGCCTGGA
CGAGTATTGGTACTACAGTGTCTTCACTCTGTCCATGCTGGTGGCTTTCGAAGCTTCCCTAGTGCAGCAG
CAGATGAGGAACATGTCAGAGATCCGAAAGATGGGCAACAAGCCCCATATGATACAGGTCTACCGCAGCC
GAAAGTGGAGGCCTGTCGCCAGTGATGACATTGTTCCTGGGGACATCGTGTCTATCGGCCGCTCGCCCCA
GGAGAACTTGGTGCCCTGTGATGTACTGCTGCTACGGGGCCGCTGCATTGTAGACGAGGCCATGCTCACT
GGGGAATCTGTGCCACAAATGAAGGAGCCCATTGAAGACCTGAGCCCAGACCGTGTCCTAGACCTGCAGG
CCGATGCTCGGCTGCATGTCATCTTTGGGGGCACCAAGGTGGTACAGCACATTCCCCCGCAGAAGGCCAC
CTCGGGCCTGAAGCCGGTCGACAATGGATGTGTGGCCTTTGTCCTGAGAACTGGGTTCAACACATCCCAG
GGCAGACTACTGCGTACAATTCTCTTTGGGGTAAAGAGGGTCACTGCAAACAACTTGGAGACCTTTATCT
TCATCCTCTTCCTCCTGGTCTTCGCCATTGCTGCAGCTGCGTATGTGTGGGTTGAAGGGACCAAGGACCC
CAGCCGGAACCGTTACAAGCTCTTTCTGGAGTGCACCCTCATCCTCACCTCTGTTGTACCCCCTGAACTG
CCCATTGAACTGTCTCTGGCTGTCAACACCTCCCTCATCGCCTTGGCCAAGCTCTACATGTACTGCACAG
AGCCTTTCCGGATCCCCTTCGCTGGCAAGGTTGAAGTGTGTTGCTTTGACAAGACGGGTACCTTGACCAG
TGACAGCCTGGTGGTGCGAGGCGTGGCAGGGCTGAGAGATGGAAAAGAGGTGACCCCAGTGTCCAGCATT
CCCATAGAAACACACCGGGCCCTGGCCTCCTGCCACTCGCTCATGCAGCTAGATGACGGCACCCTAGTGG
GTGACCCTCTGGAAAAGGCCATGCTGACAGCTGTGGACTGGACACTGACCAAAGATGAGAAAGTATTCCC
CCGAAGTATTAAAACTCAGGGGCTGAAAATTCACCAGCGCTTTCATTTTGCCAGTGCCCTAAAGCGAATG
TCTGTGCTCGCCTCCTACGAGAAGCTTGGCTCCACTGACCTCTGCTACATCGCGGCTGTGAAGGGGGCCC
CCGAAACCCTGCACTCTATGTTTTCTCAGTGCCCACCTGACTACCACCACATCCACACTGAGATCTCTCG
GGAAGGAGCCCGTGTCCTGGCTCTGGGATACAAGGAGCTAGGACATCTCACACATCAGCAGGCCCGGGAG
ATCAAGCGGGAGGCACTGGAGTGCAGCCTCAAGTTTGTGGGCTTCATCGTGGTCTCCTGTCCCCTCAAAG
CTGACTCCAAGGCTGTGATCCGAGAGATCCAGAATGCATCCCACAGGGTGGTCATGATCACAGGAGACAA
CCCCCTCACTGCATGCCATGTAGCTCAGGAGCTGCACTTCATTGACAAAGCCCACACGCTCATCTTGCAT
CCTCCCTCGGAGAAAGGCCAGCCATGTGAGTGGCGCTCCATTGATAGCAGCATCGTTCTGCCTCTCACCC
TGGGCTCCCCCAAGGCACTGGCCCTGGAGCACGCGCTATGCCTCACAGGCGATGGCTTGGCTCACCTGCA
GGCTGTGGACCCCCAGCAGCTGCTCTGCCTCATCCCTCATGTGCAGGTGTTTGCCCGTGTAGCCCCTAAG
CAGAAGGAATTTGTCATCACTAGCCTGAAGGAGCTGGGTTATGTGACCCTTATGTGTGGAGATGGCACCA
ACGATGTGGGCGCACTGAAGCACGCTGATGTGGGTGTAGCACTCCTGGCCAATGCCCCTGAGAGGGTTGT
AGAACGGCGGCGACGGCCAAGGGACAGCCCTGTCCTGAGCAACAGTGGCCCCAGAGTCTCCAGGTCCACC
AAACAGAAGTCGGCACTCCTCTCTCCTGAGGAGCCACCAGCTTCCCACAGGGACCGCCTGAGCCAGGTGC
TGCGGGACCTGGAGGAGGAGAGCACCCCCATCGTGAAGCTGGGGGATGCCAGCATCGCAGCACCCTTCAC
CTCCAAGCTGTCCTCCATCCAGTGCATCTGCCACGTGATCAAGCAGGGCCGGTGCACATTGGTGACGACG
CTGCAGATGTTCAAGATCCTGGCCCTCAATGCCCTCATCCTGGCATACAGCCAAAGTGTCCTCTACCTGG
AGGGTGTCAAGTTCAGTGACTTCCAGGCCACACTGCAGGGGCTGCTGCTGGCCGGCTGCTTCCTCTTCAT
CTCCCGCTCCAAGCCTCTCAAGACTCTTTCCCGAGAGCGGCCCCTACCCAACATCTTCAACCTGTACACC
ATCCTTACGGTGATGCTGCAATTCTCTGTCCACTTCCTGAGCCTGGTCTACCTGTACCGTGAAGCCCAGG
CACGCAGCCCTGAGAAGCAGGAGCAGTTTGTAGACCTGTACAAGGAGTTTGAGCCGAGCCTGGTCAACAG
CACTGTGTACATCATGGCCATGGCCATGCAGATGGCCACCTTCGCCATCAACTACAAGGGCCCACCCTTC
ATGGAGAGCCTGCCTGAGAACAAGCCCCTGGTGTGGAGTCTGGCAGTGTCACTGCTGGCCATCATTGGCT
TGCTCCTTGGTTCCTCACCTGACTTCAACAGCCAGTTTGGCCTTGTGGACATCCCTGTAGAGTTCAAGCT
GGTCATCGGCCAGGTCCTGGCCCTGGACTTCTGCCTGGCACTCCTGGCTGACCGTGTCCTACAGTTCTTT
TTGGGGACTCCGAAGCTGAGAGTGCCTTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216408 representing NM_133224
Red=Cloning site Green=Tags(s)

MAVVGNAVPCGARPGGARDNGSPQPGSRLRPGLAAGPALIANGDELVAAVWPYRRLALLRRLTVLPFAGL
LYPAWLGAAASGCWGWGSSWTQIPEAALLALATICLAHALTVLSGHWSVHAHCALTCTPEYDPNKVTFVK
VVPTPNNGSTELVALHRDKGEDGLEVLSFEFQKIKYSYDALEKKQFLPVAFPVGNAFSYYQSNRGFQEDS
EIRAAEKKFGSNKAEMVVPDFSELFKERATAPFFVFQVFCVGLWCLDEYWYYSVFTLSMLVAFEASLVQQ
QMRNMSEIRKMGNKPHMIQVYRSRKWRPVASDDIVPGDIVSIGRSPQENLVPCDVLLLRGRCIVDEAMLT
GESVPQMKEPIEDLSPDRVLDLQADARLHVIFGGTKVVQHIPPQKATSGLKPVDNGCVAFVLRTGFNTSQ
GRLLRTILFGVKRVTANNLETFIFILFLLVFAIAAAAYVWVEGTKDPSRNRYKLFLECTLILTSVVPPEL
PIELSLAVNTSLIALAKLYMYCTEPFRIPFAGKVEVCCFDKTGTLTSDSLVVRGVAGLRDGKEVTPVSSI
PIETHRALASCHSLMQLDDGTLVGDPLEKAMLTAVDWTLTKDEKVFPRSIKTQGLKIHQRFHFASALKRM
SVLASYEKLGSTDLCYIAAVKGAPETLHSMFSQCPPDYHHIHTEISREGARVLALGYKELGHLTHQQARE
IKREALECSLKFVGFIVVSCPLKADSKAVIREIQNASHRVVMITGDNPLTACHVAQELHFIDKAHTLILH
PPSEKGQPCEWRSIDSSIVLPLTLGSPKALALEHALCLTGDGLAHLQAVDPQQLLCLIPHVQVFARVAPK
QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPVLSNSGPRVSRST
KQKSALLSPEEPPASHRDRLSQVLRDLEEESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTT
LQMFKILALNALILAYSQSVLYLEGVKFSDFQATLQGLLLAGCFLFISRSKPLKTLSRERPLPNIFNLYT
ILTVMLQFSVHFLSLVYLYREAQARSPEKQEQFVDLYKEFEPSLVNSTVYIMAMAMQMATFAINYKGPPF
MESLPENKPLVWSLAVSLLAIIGLLLGSSPDFNSQFGLVDIPVEFKLVIGQVLALDFCLALLADRVLQFF
LGTPKLRVPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133224
ORF Size 3600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133224.2, NP_573487.2
RefSeq Size 3915 bp
RefSeq ORF 3603 bp
Locus ID 170759
UniProt ID Q9EPE9
Cytogenetics 8 B3.3
Summary Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133224" in other vectors (4)
SKU Description Size Price
MC223923 Atp13a1 (untagged) - Mouse ATPase type 13A1 (Atp13a1), (10ug) 10 ug
$1,150.00
MR216408 Atp13a1 (Myc-DDK-tagged) - Mouse ATPase type 13A1 (Atp13a1) 10 ug
$589.00 $1,045.00
MR216408L3 Lenti ORF clone of Atp13a1 (Myc-DDK-tagged) - Mouse ATPase type 13A1 (Atp13a1) 10 ug
$1,345.00
MR216408L4 Lenti ORF clone of Atp13a1 (mGFP-tagged) - Mouse ATPase type 13A1 (Atp13a1) 10 ug
$1,345.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.