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Agap3 (NM_139153) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216384
Agap3 (tGFP-tagged) - Mouse ArfGAP with GTPase domain ankyrin repeat and PH domain 3 (Agap3), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,033.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Agap3
Synonyms AGAP-3; AV028425; Centg3; cnt-g3; Crag; Crag.; MRIP-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216384 representing NM_139153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTCCAGGCAGGCGGGGGCCAGAGTCCTCAGCAGCAGCAGAGCCTGGCGGCTCCGGGGACCGGCG
GCGGCGGCGGCGGCGGCGCGGGGGGCGGCGGGCAGTTCGGCGGCGCGGGGCCCGGAGCCGGGGGTGGCGG
CCCCTCGCAGCAGCTGGCTGGCGGCCCCCCCCAGCAGTTCGCGCTCTCCAACTCGGCCGCTATCCGAGCC
GAGATCCAACGCTTCGAGTCGGTACACCCCAACATCTACGCCATCTACGACCTGATCGAGCGAATCGAGG
ATTTGGCGCTGCAGAACCAGATCCGGGAGCACGTCATCTCCATCGAGGACTCATTTGTGAACAGCCAGGA
GTGGACCCTGAGCCGCTCTGTGCCAGAGCTTAAAGTGGGCATAGTGGGCAACCTGTCTAGTGGGAAATCA
GCCTTGGTTCACCGATATCTGACAGGGACTTATGTCCAGGAAGAGTCCCCTGAAGGGGGTCGATTTAAAA
AGGAGATTGTAGTGGATGGCCAGAGTTACCTGCTGCTGATCCGGGATGAAGGAGGCCCCCCTGAACTCCA
GTTTGCTGCCTGGGTGGATGCGGTGGTGTTTGTGTTCAGCCTGGAGGATGAAATCAGCTTCCAGACGGTG
TACAACTACTTCCTACGCCTCTGCAGCTTTCGAAATGCCAGCGAGGTGCCCATGGTGCTGGTGGGCACAC
AGGATGCCATCAGTGCCGCCAATCCCCGGGTCATTGATGACAGCAGGGCCCGCAAACTGTCCACAGACCT
GAAGCGCTGTACCTACTATGAAACATGCGCAACCTATGGGCTCAATGTGGAACGAGTCTTCCAGGACGTG
GCCCAGAAGGTGGTAGCCTTGAGGAAGAAGCAGCAGCTGGCGATCGGGCCCTGCAAGTCCCTGCCCAACT
CACCCAGCCACTCGGCCGTGTCAGCTGCCTCCATCCCAGCTGTGCACATCAATCAGGCCACCAACGGAGG
CAGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCTAGCATCAGCCAGCGGGAGCTGCGC
ATCGAGACCATCGCTGCCTCCTCCACCCCCACGCCCATCCGCAAGCAGTCCAAGCGGCGTTCTAACATCT
TCACGTCTCGAAAAGGTGCTGACCTGGACAGGGAGAAGAAAGCTGCGGAGTGCAGAGTGGACAGCATCGG
GAGTGGCCGGGCCATCCCCATCAAGCAGGGGATCCTGCTGAAAAGAAGCGGCAAATCCTTGAACAAGGAG
TGGAAGAAGAAGTATGTGACGCTGTGTGACAACGGGTTGCTTACTTACCACCCTAGCCTGCACGATTACA
TGCAAAACATCCACGGCAAAGAGATCGACCTCCTACGGACTACGGTGAAAGTGCCAGGGAAGCGTCTGCC
CCGAGCCACACCCACTACAGCCCCAGGCACCAGTCCCCGTGCCAACGGGTTGGCAATGGAGCGGAGTAAC
ACACAGCTGGGTGGGGCCACAGGTGCCCCCCACTCGGCCAGCAGCACATCCCTGCATTCTGAGCGCCCTC
ACAGCTCAGCCTGGGCTGGCTCACGCCCCGGCCCCGAGGGGCTGCACCAGCGCTCCTGTTCGGTTTCCAG
CACTGACCAGTGGAGTGAGGCAGCTGCCCTGCCTGCCAGCATGCAGCACCCTGCCAGTGGCCCAGCTGAG
TCACTCAGTTCCAGCCCCAAGCTGGAGCCTCCCCCATCTCCCCACTCCAACCGGAAGAAGCACCGCGGGA
AAAAGAGCACCGGGACCCCCCGACCAGACGGCCCCAGCAGTGCTGCTGAAGAGGCAGAGGAGTCCTTTGA
GTTTGTAGTGGTGTCCCTCACTGGTCAGACCTGGCATTTTGAGGCCTCAACAGCAGAAGAGCGGGAACTA
TGGGTGCAGAGCGTGCAGGCCCAAATCCTCGCCAGCCTACAAGGATGTCGCAGCGCCAAGGACAAGACTC
GGCTGGGGAACCAGAACACAGCCCTGGCGGTGCAGGCTGTCCGCACGGTCCGCGGCAACAGCCTCTGTAT
TGACTGTGAAGCCCCCAATCCAGACTGGGCCAGCCTGAATCTGGGTGCCCTGATGTGTATCGAGTGCTCA
GGGATCCACCGACACCTGGGAGCTCACCTGTCCCGAGTCCGCTCCTTGGACCTTGACGACTGGCCACCTG
AACTGCTGGCCGTCATGACTGCAATGGGCAACGCCTTGGCTAACAGTGTCTGGGAGGGGGCTCTGGATGG
TTACTCTAAGCCAGGCCCCGAAGCCTGCAGAGAGGAGAAAGAGCGCTGGATCCGAGCCAAGTATGAGCAG
AAGCTCTTCTTGGCCCCGCTGCCAAGCTCAGATGTGCCGCTGGGACAGCAGTTACTCCGGGCTGTGGTGG
AGGATGACCTGCGGCTGCTGGTGATGCTGCTGGCCCACGGGTCCAAGGAGGAGGTGAACGAGACATACGG
GGACGGTGACGGGAGGACAGCCCTGCACCTGTCCAGTGCCATGGCCAATGTGGTGTTCACACAGCTGCTC
ATCTGGTACGGGGTGGACGTGAGGAGCCGTGATGCCCGAGGACTGACCCCACTGGCCTATGCCCGTCGTG
CCGGCAGCCAGGAGTGTGCAGACATCTTGATCCAGCATGGGTGTCCCGGGGAAGGCTGTGGCTTAGCACC
CACTCCCAACAGAGAGCCTGCCAATGGCACCAACCCCTCCGCAGAACTGCACCGCAGCCCTAGTATCCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216384 representing NM_139153
Red=Cloning site Green=Tags(s)

MNFQAGGGQSPQQQQSLAAPGTGGGGGGGAGGGGQFGGAGPGAGGGGPSQQLAGGPPQQFALSNSAAIRA
EIQRFESVHPNIYAIYDLIERIEDLALQNQIREHVISIEDSFVNSQEWTLSRSVPELKVGIVGNLSSGKS
ALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPELQFAAWVDAVVFVFSLEDEISFQTV
YNYFLRLCSFRNASEVPMVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQDV
AQKVVALRKKQQLAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGSSAFSDYSSSVPSTPSISQRELR
IETIAASSTPTPIRKQSKRRSNIFTSRKGADLDREKKAAECRVDSIGSGRAIPIKQGILLKRSGKSLNKE
WKKKYVTLCDNGLLTYHPSLHDYMQNIHGKEIDLLRTTVKVPGKRLPRATPTTAPGTSPRANGLAMERSN
TQLGGATGAPHSASSTSLHSERPHSSAWAGSRPGPEGLHQRSCSVSSTDQWSEAAALPASMQHPASGPAE
SLSSSPKLEPPPSPHSNRKKHRGKKSTGTPRPDGPSSAAEEAEESFEFVVVSLTGQTWHFEASTAEEREL
WVQSVQAQILASLQGCRSAKDKTRLGNQNTALAVQAVRTVRGNSLCIDCEAPNPDWASLNLGALMCIECS
GIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGALDGYSKPGPEACREEKERWIRAKYEQ
KLFLAPLPSSDVPLGQQLLRAVVEDDLRLLVMLLAHGSKEEVNETYGDGDGRTALHLSSAMANVVFTQLL
IWYGVDVRSRDARGLTPLAYARRAGSQECADILIQHGCPGEGCGLAPTPNREPANGTNPSAELHRSPSIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139153
ORF Size 2730 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139153.2, NP_631892.2
RefSeq Size 3207 bp
RefSeq ORF 2733 bp
Locus ID 213990
Cytogenetics 5 A3
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Documents
Resources
"NM_139153" in other vectors (4)
SKU Description Size Price
MC222551 Agap3 (untagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (Agap3), (10ug) 10 ug
$917.00
MR216384 Agap3 (Myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (Agap3) 10 ug
$589.00 $833.00
MR216384L3 Lenti ORF clone of Agap3 (Myc-DDK-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (Agap3) 10 ug
$1,133.00
MR216384L4 Lenti ORF clone of Agap3 (mGFP-tagged) - Mouse ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (Agap3) 10 ug
$1,133.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.