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Klhl25 (NM_182782) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216374
Klhl25 (tGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $749.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl25
Synonyms 2810402K13Rik; BC027373; Enc-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216374 representing NM_182782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGTCAGTGTTCACGAGACCCGCAAGTCGAGGAGCAGCACGGGCTCCATGAACATCTCAGTCTTCC
ACAAGGCTTCACACCCTGACTGTGTGCTGGCCCACCTCAACACTCTGCGCAAGCACTGCATGTTCACGGA
TGTCACACTCTGGGCTGGTGACCGAGCCTTTCCTTGCCATCGTGCGGTGCTGGCTGCCTCCAGCCGATAC
TTTGAGGCCATGTTCAGCCACGGCCTGCGGGAAAGCCGGGATGATACGGTCAACTTCCAGGACAACCTGC
ACCCCGAGGTGTTGGAGCTGCTGTTGGACTTTGCCTACTCTTCTCGCATCGTCATCAATGAGGAGAACGC
GGAGTCACTACTAGAGGCTGGTGACATGCTGCAGTTCCATGATGTCCGGGATGCAGCTGCTGAGTTCCTG
GAGAAGAATCTTTCCCCCTCCAACTGCCTTGGCATGATGGTGCTGTCTGATGCCCACCAGTGCCGACGGC
TCTACGAGTTCTCATGCCGAATGTCCCTGGTGCATTTTGAGACGGTGCGGCAGAGCGAGGACTTCAACAG
TCTCTCTAGGGACACGCTGTTGGACCTCATCTCCAGAGACGAGCTGGAGACTGAGGATGAGCGCGTGGTC
TTTGAGGCCATCCTGCAGTGGGTGAAGCATGACTTAGAGCAGAGGAAGGCCCACCTGCCCCTGCTCCTGC
GTAACGTGCGACTGGCCCTGCTGCCCTCAGACTGCCTGAAGAATGCCGTATCTGGAGAGGCCCTCCTCAT
GGCAGACGAGTGCACCAAGCTCATCTTAGACGAGGCCTTCCGCTGCAAGACCAAGATCTTGCTGAATGAT
GGTGTGGTCACCAGTCCCTTTGCCCGGCCTCGCAAGGCAGGTCACACACTGCTCATCTTGGGAGGCCAGA
CCTTCATGTGTGACAAGATCTACCAAGTGGACCACAAAGCCAAGGAGATTATCCCCAAGGCAGATCTGCC
AAGCCCCCGGAAGGAGTTCAGTGCTTCAGCGATTGGTTGCAAGGTCTATGTGACTGGAGGCAGGGGCTCT
GAGAATGGAGTCTCTAAGGATGTCTGGGTGTATGACACTGTCCATGAGGAATGGTCTAAGGCCGCCCCTA
TGCTGATTGCCCGCTTTGGCCATGGCTCAGCCGAGCTGGAGAACTGTCTCTATGTGGTGGGGGGGCATAC
ATCCCTAGCAGGTATTTTCCCTGCCTCCCCTTCTGTCTCCCTGAAGCAAGTAGAGAAATATGACCCTGGG
GATAATAAGTGGACTATGGTGGCCCCCATGAGAGACGGTGTCAGCAATGCTGCAGTGGTGAGTGCCAAGC
TGAAACTCTTTGTGTTTGGAGGGACCAGCATCCACCGAGATATGGTGTCCAAAGTCCAGTGTTTTGACCC
CTCGGAGAACCGGTGGACAATCAAAGCAGAGTGTCCCCAGCCCTGGCGATACACAGCCGCCGCTGTCCTG
GGCAGCCAGATCTTCATCATGGGAGGTGATACAGAGTACACAGCAGCTTCAGCCTACCGCTTCGACTGTG
AGACCAACCAGTGGACGCGGATTGGGGACATGACAGCCAAACGCATGTCCTGTCATGCCGTGGCTTCAGG
CAACAAGCTGTATGTGGTGGGAGGCTACTTTGGGACCCAGAGGTGTAAGACCCTGGACTGCTATGACCCT
ACTTCAGACACATGGAACTGCATCACCACTGTGCCCTACTCTCTCATTCCCACGGCCTTTGTTAGCACCT
GGAAGCATCTGCCTGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216374 representing NM_182782
Red=Cloning site Green=Tags(s)

MSVSVHETRKSRSSTGSMNISVFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCHRAVLAASSRY
FEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIVINEENAESLLEAGDMLQFHDVRDAAAEFL
EKNLSPSNCLGMMVLSDAHQCRRLYEFSCRMSLVHFETVRQSEDFNSLSRDTLLDLISRDELETEDERVV
FEAILQWVKHDLEQRKAHLPLLLRNVRLALLPSDCLKNAVSGEALLMADECTKLILDEAFRCKTKILLND
GVVTSPFARPRKAGHTLLILGGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGS
ENGVSKDVWVYDTVHEEWSKAAPMLIARFGHGSAELENCLYVVGGHTSLAGIFPASPSVSLKQVEKYDPG
DNKWTMVAPMRDGVSNAAVVSAKLKLFVFGGTSIHRDMVSKVQCFDPSENRWTIKAECPQPWRYTAAAVL
GSQIFIMGGDTEYTAASAYRFDCETNQWTRIGDMTAKRMSCHAVASGNKLYVVGGYFGTQRCKTLDCYDP
TSDTWNCITTVPYSLIPTAFVSTWKHLPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182782
ORF Size 1767 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182782.1
RefSeq Size 2314 bp
RefSeq ORF 1770 bp
Locus ID 207952
UniProt ID Q8R2P1
Cytogenetics 7 D1
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_182782" in other vectors (5)
SKU Description Size Price
MC202166 Klhl25 (untagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3, (10ug) 10 ug
$549.00
MG209189 Klhl25 (tGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25) 10 ug
$749.00
MR216374 Klhl25 (Myc-DDK-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 10 ug
$289.00 $549.00
MR216374L3 Lenti ORF clone of Klhl25 (Myc-DDK-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 10 ug
$849.00
MR216374L4 Lenti ORF clone of Klhl25 (mGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 10 ug
$849.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.