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Tmtc3 (NM_001110013) Mouse Tagged ORF Clone

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SKU
MG216360
Tmtc3 (tGFP-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,042.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmtc3
Synonyms 9130014E20Rik; B130008E12Rik; mSmile
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216360 representing NM_001110013
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGAAGGGAAGATGGCTGATATTAACTTCAAAGAAGTGACTTTAATAGTCAGCGTGGTTGCCGCCT
GCTACTGGAACAGCCTGTTCTGTGGGTTTGTTTTTGATGATGTTTCAGCAATATTGGATAATAAAGACCT
GCATCCATCTACACCTTTAAAAACTTTATTTCAGAATGACTTCTGGGGAACTCCTATGTCTGAGGAGAGA
AGCCACAAGTCATACAGGCCCTTAACGGTGTTGACATTTCGCTTAAATTATCTCCTCAGTGAGCTGAAAC
CCATGTCTTACCACCTCCTGAACACAGTGTTCCACGCTGTCGTCAGCGTGATCTTCCTTAAGGTCTGCAG
ACTCTTTCTGGATAAGAGGAGCAGCATGATTGCGGCTCTGCTCTTCGCAGTGCACCCAATCCATACCGAG
GCAGTCACAGGTGTTGTAGGAAGAGCGGAACTCTTATCATCTGTCTTTTTCCTAGCTGCCTTTCTCTCGT
ATACTAAGTCAAAAGGACCAGATAATTCCATAGTGTGGACCCCGATTGTATTAACCGTGTTTTTAGTGGC
TGTTGCAACATTGTGTAAGGAACAAGGAATAACGGTTGTCGGAATTTGTTGTGTATATGAAGTATTTGTG
GCCCAAGGGTACACCTTGCCGATGTTATGCACCGTTGCCGGGCAGTTTCTGCGCGGGAAGGGCAGCATTC
CGTTATCTATGCTGCAGACACTGGTGAAGCTCATTGTCCTGATGCTCAGTACCTTGCTACTCGTTGTGGT
CAGAGTCCAAGTTATTCAGTCACAACTTCCAGTGTTTACCAGGTTTGATAATCCAGCTGCTGTAAGCCCA
ACCCCGACACGACAGCTGACTTTTAACTATCTCCTTCCTGTGAATGCCTGGCTTCTGCTGAATCCTTCGG
AGCTCTGCTGTGATTGGACCATGGGGACAATACCACTGATAGAATCATTTCTAGATGTTCGAAATCTCGC
CACTTTTGCTTTCTTTTGCTTTCTGGGGGCTTTGGGAATATTCAGTCTCAGATACCCTGGTGACTCCTCA
AAGACTGTCCTAATGGCGCTTTGTTTAATGGCGTTACCATTTATTCCCGCATCAAACCTGTTCTTTCCTG
TTGGATTTGTGGTTGCTGAGCGAGTACTATATGTTCCTAGCATGGGGTTCTGTATTTTAGTAGCCCATGG
ATGGCAAAAAATTTCAAACAAAAGTGTGCTGAAAAAGCTCTCGTGGGTTTGTCTGTCCATGGTGATACTA
ACCCATGCCTTGAAAACACTTCATAGAAATTGGGACTGGGAGTCAGAATATACATTGTTTATGTCAGCCC
TAAAGGTGAATAAAAACAATGCCAAATTATGGAATAATGTGGGTCATGCTCTGGAGAATGAGAAGAACTT
TGAGAAAGCTTTGAAATACTTCTTGCAGGCTACCCATGTTCAGCCAGATGACATCGGTGCCCACATGAAT
GTAGGAAGAACTTATAAAAACTTAAACAGAACTAGAGAAGCTGAAGCGTCTTACATGCTGGCTAAATCAC
TGATGCCTCAGATTATCCCTGGTAAAAAATATGCAGCCAGAATTGCCCCTAATCACCTAAACGTTTATAT
CAATCTGGCCAACCTTATTCGAGCAAATGAGTCCCGCCTGGAGGAAGCGGACCAGCTGTACCGACAGGCC
ATCAGCATGAGGCCAGACTTCAAACAGGCTTACATTAGCAGGGGAGAGTTGCTTTTAAAAATGAATAAGC
CTCTCAAAGCAAAGGAAGCATATCTTAAAGCACTAGAGCTGGACAGAAATAATGCAGATCTCTGGTACAA
CTTGGCAATTGTTTATATTGAACTTAAAGAACCAAATGAAGCTCTGAAGAACTTTAATCGAGCTTTGGAA
CTGAACCCTAAACATAAGCTAGCACTGTTCAATTCTGCTATTTTAATGCAGGAATCAGGTGAAGTTAAAC
TCAGACCTGAAGCTAGAAAACGGCTTCTAAATTATGTAAATGAAGAGCCACAGGATGCTAATGGCTATTT
CAATTTGGGGATGCTCGCCATGGATGACAAAAAGGATTCAGAAGCTGAGTCTTGGATGAAGAAAGCTATC
AAATTACAACCTGACTTCAGAAGCGCTTTGTTTAACCTGGCACTCCTGTATTCGCAGACTGCTAAGGAGT
TAAAGGCATTGCCAATTTTAGAAGAGCTGCTTAAATACTATCCTGACCATACCAAGGGTCTCATTTTAAA
AGGAGACATTCTGATGAACCAAAAGAAGGATATACCTGGAGCTAAAAAGTGTTTTGAAAAGATCCTGGAA
ATGGATCCAAGCAATGTGCAGGGGAAGCACAACCTGTGTGTTGTGTACTTTGAAGAGAAGGAGTTACTAA
AAGCTGAGAGGTGTCTTGTGGAAACATTGGCATTAGCACCTCATGAGGAGTATATTCAACGCCATTTGAG
TATAGTCAGGGATAGAATCTCCTCTTCTGGTATTGTGGAGCAGCCACTGGCCCCAGCTGATAAGACTCCA
GGTACAGAGGAGAGAGAGGAAATCCCTTCTGAGGATGTAAAAGAAATCAGCAGTGAATCCAGACCACCAC
AAATCTTAAAAACAAACAATAACAGAAACTCTAAATCCAACAAGCAATCAACAGAAAATGCAGACCAAGA
TGCCCCCCATAAAACAACAAAAGACATCAAAGAAATTGAGAAGAAAAGAGTTGCTGCTTTGAAAAGGCTA
GAAGAGATTGAACGTATTTTAAATGGAGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216360 representing NM_001110013
Red=Cloning site Green=Tags(s)

MLEGKMADINFKEVTLIVSVVAACYWNSLFCGFVFDDVSAILDNKDLHPSTPLKTLFQNDFWGTPMSEER
SHKSYRPLTVLTFRLNYLLSELKPMSYHLLNTVFHAVVSVIFLKVCRLFLDKRSSMIAALLFAVHPIHTE
AVTGVVGRAELLSSVFFLAAFLSYTKSKGPDNSIVWTPIVLTVFLVAVATLCKEQGITVVGICCVYEVFV
AQGYTLPMLCTVAGQFLRGKGSIPLSMLQTLVKLIVLMLSTLLLVVVRVQVIQSQLPVFTRFDNPAAVSP
TPTRQLTFNYLLPVNAWLLLNPSELCCDWTMGTIPLIESFLDVRNLATFAFFCFLGALGIFSLRYPGDSS
KTVLMALCLMALPFIPASNLFFPVGFVVAERVLYVPSMGFCILVAHGWQKISNKSVLKKLSWVCLSMVIL
THALKTLHRNWDWESEYTLFMSALKVNKNNAKLWNNVGHALENEKNFEKALKYFLQATHVQPDDIGAHMN
VGRTYKNLNRTREAEASYMLAKSLMPQIIPGKKYAARIAPNHLNVYINLANLIRANESRLEEADQLYRQA
ISMRPDFKQAYISRGELLLKMNKPLKAKEAYLKALELDRNNADLWYNLAIVYIELKEPNEALKNFNRALE
LNPKHKLALFNSAILMQESGEVKLRPEARKRLLNYVNEEPQDANGYFNLGMLAMDDKKDSEAESWMKKAI
KLQPDFRSALFNLALLYSQTAKELKALPILEELLKYYPDHTKGLILKGDILMNQKKDIPGAKKCFEKILE
MDPSNVQGKHNLCVVYFEEKELLKAERCLVETLALAPHEEYIQRHLSIVRDRISSSGIVEQPLAPADKTP
GTEEREEIPSEDVKEISSESRPPQILKTNNNRNSKSNKQSTENADQDAPHKTTKDIKEIEKKRVAALKRL
EEIERILNGE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001110013
ORF Size 2760 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001110013.1, NP_001103483.1
RefSeq Size 5990 bp
RefSeq ORF 2763 bp
Locus ID 237500
UniProt ID Q8BRH0
Cytogenetics 10 D1
Summary Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3. Involved in the positive regulation of proteasomal protein degradation in the endoplasmic reticulum (ER), and the control of ER stress response.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001110013" in other vectors (4)
SKU Description Size Price
MC222668 Tmtc3 (untagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3), transcript variant 1, (10ug) 10 ug
$927.00
MR216360 Tmtc3 (Myc-DDK-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3), transcript variant 1 10 ug
$589.00 $842.00
MR216360L3 Lenti ORF clone of Tmtc3 (Myc-DDK-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3), transcript variant 1 10 ug
$1,142.00
MR216360L4 Lenti ORF clone of Tmtc3 (mGFP-tagged) - Mouse transmembrane and tetratricopeptide repeat containing 3 (Tmtc3), transcript variant 1 10 ug
$1,142.00

file-alt Citations

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