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Adgrg5 (NM_001033468) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216358
Gpr114 (tGFP-tagged) - Mouse G protein-coupled receptor 114 (Gpr114) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $689.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adgrg5
Synonyms Gm1109; Gpr114; PGR27
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216358 representing NM_001033468
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCACACGGCGCCCTTTTCTTCTACCTGTGCCTTCTGGCTGCTCAAGTTGTCCTAGTAGAAACCC
TGTCAGACCTACTTGTCTTGATGAAGCGCCTTGAGCAGCCCGTAGGCCGGGGCTTGTCTTCCAGAGCGAG
GCATATCCACAGCCTGGAGCAGAAGCTGCTCAATGCCAGCTTCGGCGGGCACAACTTGACCTTGCAGACA
AACTCCATCCAGTCTCTTGTCTTCAAGCTGAGCTGCGACTTTCCTGGTCTTTCCCTGTCCAGCACCACAC
TGACAAATGTCTCCCAGGTGCGAGCCCCACATGCCATGCAATTCCCTGCTGAGCTGACCAAGGGTGCCTG
TGTGACCTCCCGGCCTGCTGAGCTTCGACTCATCTGCATCTATTTCTTCACTGCACACCTCTTTCAGGAT
GACCGGAACTCATCACTGCTCAATAACTATGTCCTGGGGGCCCAGCTGGATCACAGGCCTGTGAACAACC
TTCAGAAGCCAGTCAACATCAGCTTCTGGCATAATCGGAGTCTGGAAGGGTATACAGTATCCTGTGTTTT
CTGGAAGGAGGGAGCCAGCAAGAGCAGCTGGGGGGCCTGGAGCCCTGAGGGCTGTTATACAGAGCAGCCC
TCAGCCACCCAGGTTCTCTGCCATTGCAACCATCTCACCTACTTCGCTGTGCTTATGCAGCTCTCTGGAG
ACCCCGTGCCCGCTGAGCTGCAGGTGCCTCTTGAGTACATCTCCTTTGTGGGTTGCAGCATCTCCATCGT
GGCCTCGCTGCTCACCATACTGCTGTACGCTCAGTCCAGGAAGCAAAGCGATTCCACCACACGTATCCAC
ATGAACCTGAATGGCTCTGTTCTGCTCCTGAACGTCACCTTCCTTCTGAGCTCCCAGATGACCCTGCCCA
CCATGCCCAGGCCCGTCTGCAAGGTGCTGGCTGCTGTCCTACACTACGCACTGCTCAGCAGCCTTACCTG
GATGGCCATCGAAGGCTTCAACCTCTACCTTTTCCTGGGGCGTGTCTACAACGCCTACATTCGCCGATAC
TTGCTCAAGCTCTGCATGCTGGGCTGGGGGTTTCCAGCCCTCTTGGTGCTGCTGCTTCTGATGATCAAGA
GCTCAGTGTATGGACCCTGTGTGACCTCACTCTCCAAAAGCCAGGAAAATGGCACAGGCTTCCAGAATGT
GTCCATGTGCTGGATCCGAAGTCCCATGGTACACAGCATCCTGGTCATGGGCTATGGTGGCTTCACATCT
CTGTTCAACCTGGTGGTGCTGGCCTGGGCTCTGTGGATCTTGTGCAGGCTTCGGGCACGGGAGAAGGCAC
TGAGTCCCTGGGCCTACCGGGACACTGCCATGGTGTTGGGTCTCACTGTGCTGCTTGGCACCACCTGGAC
CCTAGCCTTCTTCTCCTTCGGTGTGTTCCTGCTGCCTCAGCTCTTCCTCTTTACCATCTTCAACTCGCTC
TATGGTTTCTTCCTCTTCCTGTGGTTCTGCTCACAGAAGCGTTACTCGGATGCCGAAGCCAAGGCAGAGA
TGGAGGCAGTCAGCTCCTCCCAGATGACACAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216358 representing NM_001033468
Red=Cloning site Green=Tags(s)

MDPHGALFFYLCLLAAQVVLVETLSDLLVLMKRLEQPVGRGLSSRARHIHSLEQKLLNASFGGHNLTLQT
NSIQSLVFKLSCDFPGLSLSSTTLTNVSQVRAPHAMQFPAELTKGACVTSRPAELRLICIYFFTAHLFQD
DRNSSLLNNYVLGAQLDHRPVNNLQKPVNISFWHNRSLEGYTVSCVFWKEGASKSSWGAWSPEGCYTEQP
SATQVLCHCNHLTYFAVLMQLSGDPVPAELQVPLEYISFVGCSISIVASLLTILLYAQSRKQSDSTTRIH
MNLNGSVLLLNVTFLLSSQMTLPTMPRPVCKVLAAVLHYALLSSLTWMAIEGFNLYLFLGRVYNAYIRRY
LLKLCMLGWGFPALLVLLLLMIKSSVYGPCVTSLSKSQENGTGFQNVSMCWIRSPMVHSILVMGYGGFTS
LFNLVVLAWALWILCRLRAREKALSPWAYRDTAMVLGLTVLLGTTWTLAFFSFGVFLLPQLFLFTIFNSL
YGFFLFLWFCSQKRYSDAEAKAEMEAVSSSQMTH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033468
ORF Size 1572 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033468.3, NP_001028640.2
RefSeq Size 2812 bp
RefSeq ORF 1572 bp
Locus ID 382045
UniProt ID Q3V3Z3
Cytogenetics 8 C5
Summary Adhesion G protein-coupled receptor (GPCR). Transduces intracellular signals through coupling to guanine nucleotide-binding protein G(s) subunit alpha and activation of adenylate cyclase pathway (PubMed:22575658). Isoform 1, but not isoform 2, is constitutively active, as evidenced by elevated basal cAMP levels, and responds to mechanical activation (shaking) (PubMed:26499266).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033468" in other vectors (4)
SKU Description Size Price
MC217540 Gpr114 (untagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2, (10ug) 10 ug
$537.00
MR216358 Gpr114 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 10 ug
$289.00 $489.00
MR216358L3 Lenti ORF clone of Gpr114 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 10 ug
$789.00
MR216358L4 Lenti ORF clone of Gpr114 (mGFP-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 10 ug
$789.00

file-alt Citations

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