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Atp8a1 (NM_001038999) Mouse Tagged ORF Clone

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SKU
MG216348
Atp8a1 (tGFP-tagged) - Mouse ATPase aminophospholipid transporter (APLT) class I type 8A member 1 (Atp8a1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,213.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Atp8a1 or NM_001038999"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp8a1
Synonyms AI481521; AI853962; APLT; Atp3a2; AW743152; AW822227; B230107D19Rik; ClassI
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216348 representing NM_001038999
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGACCATGCGGAGGACAGTGTCGGAGATCCGCTCGCGCGCGGAAGGTTATGAGAAGACAGATGATG
TTTCAGAGAAGACCTCGCTGGCAGATCAGGAGGAAGTGAGAACCATCTTCATCAACCAGCCTCAGCTGAC
AAAATTCTGCAACAACCACGTCAGTACTGCAAAATACAACGTGATCACATTCCTTCCGAGGTTCCTCTAT
TCTCAGTTCCGAAGAGCGGCTAATTCGTTCTTTCTCTTTATTGCCCTGCTCCAGCAAATTCCTGATGTGT
CGCCGACCGGTCGTTACACAACACTGGTTCCTCTCTTATTTATTTTAGCTGTGGCTGCTATTAAAGAGAT
AATAGAAGATATTAAACGACACAAGGCTGATAATGCTGTGAACAAGAAACAGACACAAGTTTTGAGAAAT
GGTGCATGGGAAATTGTTCACTGGGAAAAGGTGGCAGTGGGGGAGATAGTGAAGGTGACCAATGGGGAAC
ACCTCCCGGCAGATCTCCTCAGTCTGTCCTCAAGTGAGCCCCAGGCCATGTGCTACATCGAGACATCCAA
CTTAGATGGTGAAACAAACTTGAAAATTAGACAAGGCTTACCGGCAACATCGGATATCAAAGACATTGAC
AGTTTGATGAGAATTTCTGGCAGAATCGAGTGTGAAAGTCCAAACCGACATCTCTACGATTTTGTTGGGA
ACATAAGGCTTGATGGCCATGGCACCGTTCCCCTGGGGGCAGACCAGATCCTTCTCCGAGGAGCTCAGTT
AAGAAATACCCAGTGGGTTCATGGGATAGTTGTCTACACTGGCCACGACACCAAGCTGATGCAGAATTCC
ACAAGCCCACCACTTAAACTCTCCAATGTGGAACGGATTACAAATGTACAAATTCTGATTTTATTTTGCA
TCTTAATTGCCATGTCTCTTGTGTGTTCTGTGGGCTCAGCCATTTGGAACCGAAGGCATTCCGGGAAAGA
CTGGTACCTCCATCTACACTATGGTGGCGCTAGTAACTTTGGACTGAACTTCCTGACTTTCATTATCCTT
TTCAACAACCTCATTCCCATCAGCTTGCTGGTCACATTAGAAGTGGTGAAGTTTACTCAGGCATACTTCA
TAAATTGGGATCTTGACATGCATTATGAGCCCACAGACACCGCAGCAATGGCTCGGACATCTAATCTGAA
TGAGGAACTTGGCCAGGTTAAATACATATTTTCTGACAAAACTGGGACCCTGACATGCAATGTGATGCAG
TTCAAGAAGTGCACCATCGCGGGCGTGGCCTATGGCCATGTCCCTGAACCTGAGGATTATGGGTGCTCGC
CCGATGAATGGCAGAGCTCACAGTTTGGAGATGAAAAAACCTTTAATGACCCGTCGTTGCTGGACAATCT
CCAGAATAACCACCCAACCGCACCTATCATCTGTGAATTTCTCACAATGATGGCCGTCTGCCACACAGCT
GTACCGGAGAGAGAAGGGGACAAGATCATTTATCAGGCTGCATCGCCAGATGAGGGTGCACTGGTCAGAG
CCGCCAAGCAGTTGAATTTTGTCTTCACTGGAAGAACTCCTGACTCTGTCATCATAGATTCACTGGGGCA
GGAAGAAAGATATGAATTGCTCAATGTCCTAGAGTTCACCAGTGCTAGGAAGAGGATGTCGGTGGTGGTT
CGCACTCCATCCGGGAAGTTACGGCTCTACTGCAAAGGAGCTGACACAGTAATTTATGAACGACTTGCAG
AGACCTCAAAGTACAAAGAAATCACCCTAAAACACTTGGAACAGTTTGCTACAGAAGGGCTGAGGACTTT
GTGTTTTGCTGTGGCTGAGATTTCTGAGAGCGACTTCGAGGAGTGGCGGGCCGTCTACCACCGCGCATCC
ACGTCGGTACAGAACAGGCTGCTGAAGCTGGAGGAGAGCTACGAACTAATTGAAAAGAATCTTCAGCTAC
TTGGAGCTACAGCCATTGAGGATAAATTGCAGGACCAAGTGCCTGAAACCATAGAAACGCTAATGAAGGC
TGACATCAAAATATGGATCCTTACTGGGGACAAGCAAGAAACTGCCATTAATATTGGACACTCCTGTAGA
CTCCTCAAGAGGAACATGGGAATGATCGTGATAAACGAGGGCTCTCTTGACGGCACGAGGGAAACTCTCA
GCCGCCACTGCACCACCCTGGGAGATGCTCTTCGGAAGGAAAATGATTTTGCTCTTATAATTGATGGGAA
GACCCTCAAATATGCCTTAACCTTTGGCGTCCGGCAGTATTTCCTGGACTTAGCTCTGTCCTGCAAAGCT
GTCATTTGCTGCAGGGTTTCTCCTCTTCAGAAGTCCGAGGTTGTCGAGATGGTTAAGAAACAAGTCAAAG
TCATCACACTTGCCATCGGGGATGGAGCAAATGACGTCAGCATGATCCAGACGGCCCATGTGGGTGTTGG
GATAAGCGGCAATGAAGGTTTGCAGGCAGCCAACTCTTCAGATTACTCCATCGCTCAGTTCAAATATTTG
AAGAATTTGTTGATGGTTCACGGTGCCTGGAATTACAATAGAGTCTCCAAGTGTATCCTGTACTGCTTCT
ACAAGAACATCGTGCTCTACATCATCGAGATCTGGTTTGCCTTTGTCAACGGCTTTTCTGGACAGATCCT
CTTTGAAAGATGGTGTATAGGGCTTTATAATGTGATGTTCACAGCGATGCCTCCCTTGACACTTGGAATA
TTTGAGAGATCGTGCAGAAAGGAGAACATGTTGAAGTATCCTGAGTTGTACAAAACATCCCAGAATGCTC
TGGACTTCAATACCAAGGTTTTCTGGGTTCATTGTTTGAATGGCCTCTTCCACTCCGTTATTCTGTTTTG
GTTCCCACTGAAAGCCCTGCAGTATGGCACGGTATTTGGAAATGGGAAAACCTCAGATTACCTGCTTCTG
GGAAACTTTGTTTATACTTTTGTAGTGATAACTGTGTGCTTGAAAGCTGGACTGGAAACCTCCTATTGGA
CATGGTTCAGCCACATCGCCATCTGGGGCAGCATCGCGCTCTGGGTGGTGTTCTTCGGGATCTACTCATC
TCTGTGGCCTGCCGTGCCCATGGCCCCTGACATGTCCGGAGAGGCAGCCATGCTCTTCAGCTCTGGAGTC
TTCTGGGTGGGCTTGCTCTCCATCCCTGTGGCGTCCTTGCTTCTGGATGTGCTGTACAAAGTCATCAAGA
GGACGGCCTTTAAAACCTTAGTGGATGAAGTTCAGGAGCTTGAGGCGAAATCTCAAGACCCGGGCGCAGT
CGTACTTGGAAAGAGCCTCACGGAGAGAGCGCAGCTGCTCAAGAACGTCTTTAAGAAGAACCACGTGAAT
CTGTACCGCTCCGAGTCCCTGCAGCAGAACCTGCTTCACGGCTATGCTTTCTCTCAAGATGAAAACGGCA
TCGTCTCACAGTCTGAAGTCATCAGAGCCTATGACACCACGAAACAGAGGCCCGATGAGTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216348 representing NM_001038999
Red=Cloning site Green=Tags(s)

MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQPQLTKFCNNHVSTAKYNVITFLPRFLY
SQFRRAANSFFLFIALLQQIPDVSPTGRYTTLVPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRN
GAWEIVHWEKVAVGEIVKVTNGEHLPADLLSLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDID
SLMRISGRIECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKLMQNS
TSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLHLHYGGASNFGLNFLTFIIL
FNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMARTSNLNEELGQVKYIFSDKTGTLTCNVMQ
FKKCTIAGVAYGHVPEPEDYGCSPDEWQSSQFGDEKTFNDPSLLDNLQNNHPTAPIICEFLTMMAVCHTA
VPEREGDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVVV
RTPSGKLRLYCKGADTVIYERLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFEEWRAVYHRAS
TSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIWILTGDKQETAINIGHSCR
LLKRNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDFALIIDGKTLKYALTFGVRQYFLDLALSCKA
VICCRVSPLQKSEVVEMVKKQVKVITLAIGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYL
KNLLMVHGAWNYNRVSKCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGI
FERSCRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTVFGNGKTSDYLLL
GNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWPAVPMAPDMSGEAAMLFSSGV
FWVGLLSIPVASLLLDVLYKVIKRTAFKTLVDEVQELEAKSQDPGAVVLGKSLTERAQLLKNVFKKNHVN
LYRSESLQQNLLHGYAFSQDENGIVSQSEVIRAYDTTKQRPDEW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001038999
ORF Size 3492 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001038999.2, NP_001034088.1
RefSeq Size 8175 bp
RefSeq ORF 3495 bp
Locus ID 11980
UniProt ID P70704
Cytogenetics 5 C3.1
Summary Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells; the activity is associated with hippocampus-dependent learning.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001038999" in other vectors (4)
SKU Description Size Price
MC223789 Atp8a1 (untagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 1, (10ug) 10 ug
$1,115.00
MR216348 Atp8a1 (Myc-DDK-tagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 1 10 ug
$589.00 $1,013.00
MR216348L3 Lenti ORF clone of Atp8a1 (Myc-DDK-tagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 1 10 ug
$1,313.00
MR216348L4 Lenti ORF clone of Atp8a1 (mGFP-tagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 1 10 ug
$1,313.00

file-alt Citations

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