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Mpzl1 (NM_001083897) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216313
Mpzl1 (tGFP-tagged) - Mouse myelin protein zero-like 1 (Mpzl1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mpzl1
Synonyms 1110007A10Rik; PZR
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216313 representing NM_001083897
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGGCCGTCGGAGCCGTGGCACTGATTGCGGCCCCGGCCCGCCGGCGCTGGCTGTGGTCGGTGC
TAGCCGCGATGCTCGGGCTGTTGACAGCTAGAATATCAGCCTTGGAGGTCCATACTCCTAAAGAAATCTT
TGTGGTGAATGGGACACAAGGGAAGCTGACTTGCACATTCGATTCTCCTAACACAACTGGATGGTTGACC
ACGGTCTCCTGGAGCTTCCAGCCAGACGGCACCGACAGCGCCGTGTCGTTTTTCCACTACTCACAAGGAC
AGGTGTACATTGGGGATTATCCACCATTTAAAGACCGAGTCACCTGGGCTGGGGACCTTGACAAGAAAGA
TGCATCAATCAATATAGAAAATATTCAGGCTGTTCACAATGGCACCTATATCTGCGATGTCAAAAATCCT
CCTGACATTGTTGTGCGGCCTGGACACATTAGGCTCCACGTGGTGGAAATAGACAACCTGTTGGTGTTCC
TGGTTTGGGTGGTGGTGGGCACTGTCACTGCTGTGGTCCTTGGCCTCACTCTGCTCATCAGCTTGGTCCT
GGTCGTCCTCTACAGAAGGAAACATTCGAAGCGGGATTATACCGGCTGCAGTACCTCAGAGCGTTTGTCA
CCAGTTAAGCAGGCTCCACGGAAGTGTCCCTCCGACACAGAGGGTCTAGTAAAGAGTCCGCCTTCCGCCG
GATCTCACCAGGGCCCAGTCATTTACGCACAGTTAGACCACTCTGGCGGACACCACAGCGGCAAGATTAA
TAAGTCAGAGTCGGTTGTGTATGCGGACATCCGGAAAGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216313 representing NM_001083897
Red=Cloning site Green=Tags(s)

MAEAVGAVALIAAPARRRWLWSVLAAMLGLLTARISALEVHTPKEIFVVNGTQGKLTCTFDSPNTTGWLT
TVSWSFQPDGTDSAVSFFHYSQGQVYIGDYPPFKDRVTWAGDLDKKDASINIENIQAVHNGTYICDVKNP
PDIVVRPGHIRLHVVEIDNLLVFLVWVVVGTVTAVVLGLTLLISLVLVVLYRRKHSKRDYTGCSTSERLS
PVKQAPRKCPSDTEGLVKSPPSAGSHQGPVIYAQLDHSGGHHSGKINKSESVVYADIRKD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001083897
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001083897.1, NP_001077366.1
RefSeq Size 2356 bp
RefSeq ORF 813 bp
Locus ID 68481
UniProt ID Q3TEW6
Cytogenetics 1 H2.3
Summary Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 2 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2, may be involved in regulation of integrin-mediated cell motility (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001083897" in other vectors (3)
SKU Description Size Price
MR216313 Mpzl1 (Myc-DDK-tagged) - Mouse myelin protein zero-like 1 (Mpzl1), transcript variant 1 10 ug
$289.00 $330.00
MR216313L3 Lenti ORF clone of Mpzl1 (Myc-DDK-tagged) - Mouse myelin protein zero-like 1 (Mpzl1), transcript variant 1 10 ug
$630.00
MR216313L4 Lenti ORF clone of Mpzl1 (mGFP-tagged) - Mouse myelin protein zero-like 1 (Mpzl1), transcript variant 1 10 ug
$630.00

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