Nek9 (NM_145138) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG216306
Nek9 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug)
$1,551.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nek9 |
| Synonyms | C130021H08Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_145138 |
| ORF Size | 2952 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_145138.2, NP_660120.2 |
| RefSeq Size | 5403 bp |
| RefSeq ORF | 2955 bp |
| Locus ID | 217718 |
| UniProt ID | Q8K1R7 |
| Cytogenetics | 12 D1 |
| Summary | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_145138" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC222965 | Nek9 (untagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9), (10ug) | 10 ug |
$902.00
|
|
| MR216306 | Nek9 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9) | 10 ug |
$1,351.00
|
|
| MR216306L3 | Lenti ORF clone of Nek9 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9) | 10 ug |
$1,651.00
|
|
| MR216306L4 | Lenti ORF clone of Nek9 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 9 (Nek9) | 10 ug |
$1,651.00
|
|
Plasmid Map for MG216306
Download sequence for
Cloning scheme for MG216306
Circular map for MG216306
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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