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Snx27 (NM_001082484) Mouse Tagged ORF Clone

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SKU
MG216284
Snx27 (tGFP-tagged) - Mouse sorting nexin family member 27 (Snx27) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $702.00
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Other products for "Snx27 or NM_001082484"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Snx27
Synonyms 5730552M22Rik; ESTM45; ESTM47; R75405
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001082484
ORF Size 1617 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001082484.1, NP_001075953.1
RefSeq Size 2321 bp
RefSeq ORF 1620 bp
Locus ID 76742
UniProt ID Q3UHD6
Cytogenetics 3 F2.1
Summary Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.UniProtKB/Swiss-Prot Function
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"NM_001082484" in other vectors (4)
SKU Description Size Price
MC218486 Snx27 (untagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 1, (10ug) 10 ug
$553.00
MR216284 Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 1 10 ug
$289.00 $502.00
MR216284L3 Lenti ORF clone of Snx27 (Myc-DDK-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 1 10 ug
$802.00
MR216284L4 Lenti ORF clone of Snx27 (mGFP-tagged) - Mouse sorting nexin family member 27 (Snx27), transcript variant 1 10 ug
$802.00

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