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Poli (NM_011972) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216260
Poli (tGFP-tagged) - Mouse polymerase (DNA directed) iota (Poli) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $876.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Poli
Synonyms Rad30b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216260 representing NM_011972
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTGGAGTCGGAGGAGGAAGGCGGCCCCGCGGAGGAAGAAGACGCTCCTCGTGCCATGGAGCCCT
TGCACGCGGGGGCGGCGGGCAGCTCGCGGGCAGTTTGCAGTCAAGGGCCACCTACACAAATCTCTTCGTC
GAGAGTCATAGTCCACGTAGATCTGGATTGCTTTTATGCCCAGGTCGAAATGATCTCCAACCCAGAATTA
AAGGACAGACCTTTAGGTGTGCAACAGAAATACTTGGTGGTTACCTGCAACTATGAAGCCAGGAAACTGG
GTGTGAGGAAGCTTATGAATGTGAGAGATGCGAAGGAAAAGTGTCCTCAGCTGGTCCTCGTGAACGGAGA
AGACCTGAGCCGCTACAGAGAGATGTCCTATAAGGTCACAGAATTGTTGGAGGAATTTAGTCCAGCTGTT
GAGAGACTTGGATTTGATGAAAATTTTGTGGATCTAACAGAAATGGTGGAAAAAAGACTCCAGCAGCTTC
CAAGTGAGGAGGTTCCGTCAGTGACTGTGTTCGGCCATGTTTACAATAACCAGTCTGTGAACCTACACAA
CATCATGCATCGGAGACTTGTGGTTGGATCGCAGATTGCAGCAGAGATGCGGGAAGCCATGTATAATCAG
CTCGGGCTCACAGGCTGCGCCGGAGTGGCTCCTAATAAACTCTTGGCAAAGTTAGTGTCTGGGGTTTTTA
AACCAAATCAGCAGACGGTCTTACTACCTGAAAGTTGTCAACATCTCATTCACAGTTTGAACCACATAAA
GGAAATTCCTGGTATCGGCTATAAAACTGCCAAGCGTCTCGAAGTTCTGGGAATCAATAGTGTTCATGAT
CTCCAAACCTTTCCAATCAAAACATTAGAAAAAGAATTAGGAATTGCAATTGCTCAGCGTATCCAGCAGC
TCAGTTTTGGAGAGGACAAGTCTCCCGTCACACCGTCAGGGCCACCACAGTCCTTTAGTGAAGAAGATAC
GTTTAAAAAGTGTTCCTCAGAAGTGGAAGCTAAAGCTAAGATTGAAGAATTACTTTCCAGCCTTTTGACC
AGAGTATGCCAGGATGGAAGGAAGCCCCATACAGTAAGATTAGTCATCCGTCGGTACTCTGACAAACACT
GTAATCGAGAGAGTCGTCAGTGCCCTATCCCATCCCACGTCATTCAGAAGTTAGGGACAGGAAATCATGA
CTCCATGCCTCCCCTGATTGATATCCTTATGAAACTTTTCCGAAATATGGTGAACGTGAAGATGCCCTTT
CACCTGACTCTTATGAGCGTGTGCTTCTGCAACCTGAAAGCCCTGAGCAGTGCTAAGAAAGGGCCTATGG
ACTGCTACCTAACGTCCCTCTCAACCCCTGCCTACACCGACAAGCGCGCCTTTAAAGTGAAGGACACCCA
CACGGAAGACTCTCACAAGGAGAAAGAAGCAAACTGGGATTGTCTACCAAGTAGAAGAATCGAAAGCACA
GGAACGGGGGAGTCTCCGTTGGATGCCACCTGTTTTCCTAAAGAAAAAGACACAAGTGACTTGCCCCTCC
AGGCACTGCCAGAGGGTGTTGATCAAGAAGTCTTTAAGCAACTTCCAGCAGATATTCAAGAAGAAATCCT
TTCTGGAAAATCTAGAGAAAATCTAAAAGGGAAAGGAAGTTTAAGTTGTCCGCTGCATGCCTCTAGAGGA
GTATTGTCTTTCTTTTCTACAAAGCAAATGCAAGCCAGTCGCTTAAGCCCCAGAGATACTGCGCTCCCTA
GCAAGCGGGTATCAGCTGCATCTCCCTGTGAGCCGGGAACGTCAGGACTGAGCCCCGGGAGCACCTCCCA
TCCATCGTGCGGAAAGGACTGTTCCTATTACATAGACAGCCAGTTAAAGGATGAGCAAACGAGTCAAGGC
CCTACTGAGTCTCAAGGATGCCAGTTTTCCAGCACGAACCCTGCTGTTTCTGGTTTCCATTCCTTTCCTA
ATCTGCAGACTGAACAGCTCTTCTCCACACATCGCACTGTAGACAGCCACAAGCAGACAGCCACCGCCTC
TCACCAGGGACTAGAAAGTCACCAGGGACTAGAAAGCAGAGAGCTGGATTCTGCGGAAGAAAAGCTTCCT
TTCCCACCAGACATTGATCCGCAAGTTTTCTATGAGCTTCCAGAAGAGGTCCAAAAGGAACTGATGGCGG
AATGGGAGAGAGCTGGAGCTGCGCGCCCCTCGGCGCACAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216260 representing NM_011972
Red=Cloning site Green=Tags(s)

MGVESEEEGGPAEEEDAPRAMEPLHAGAAGSSRAVCSQGPPTQISSSRVIVHVDLDCFYAQVEMISNPEL
KDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAV
ERLGFDENFVDLTEMVEKRLQQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQ
LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINSVHD
LQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKAKIEELLSSLLT
RVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVKMPF
HLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIEST
GTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILSGKSRENLKGKGSLSCPLHASRG
VLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKDEQTSQG
PTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLESHQGLESRELDSAEEKLP
FPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011972
ORF Size 2211 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011972.2, NP_036102.2
RefSeq Size 2482 bp
RefSeq ORF 2214 bp
Locus ID 26447
Cytogenetics 18 E2
Summary Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011972" in other vectors (4)
SKU Description Size Price
MC221231 Poli (untagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2, (10ug) 10 ug
$743.00
MR216260 Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 10 ug
$589.00 $676.00
MR216260L3 Lenti ORF clone of Poli (Myc-DDK-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 10 ug
$976.00
MR216260L4 Lenti ORF clone of Poli (mGFP-tagged) - Mouse polymerase (DNA directed), iota (Poli), transcript variant 2 10 ug
$976.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.