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Atp2a3 (NM_001163337) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216250
Atp2a3 (tGFP-tagged) - Mouse ATPase Ca++ transporting ubiquitous (Atp2a3) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,093.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp2a3
Synonyms Serca3; SERCA3b
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216250 representing NM_001163337
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGCGCACCTGCTCTCCGCTGCCGACGTGCTGCGTCGCTTCTCGGTGACAGCCGAGGGCGGCT
TGAGCCTGGAGCAGGTGACCGACGCGCGGGAGCGTTACGGCCCCAACGAGCTCCCGACCGAGGAAGGGAA
ATCCCTGTGGGAGCTGGTGGTGGAGCAGTTTGAGGACCTCTTGGTGCGCATCCTGCTGCTGGCAGCTCTG
GTTTCCTTTGTCCTGGCCTGGTTCGAGGAGGGTGAGGAGACCACAACGGCCTTCGTGGAGCCCCTGGTCA
TCATGCTAATTCTCGTGGCCAATGCGATCGTGGGAGTGTGGCAGGAACGCAACGCAGAGAGTGCAATTGA
GGCCTTGAAGGAGTATGAGCCTGAGATGGGTAAGGTGATCCGGTCTGACCGCAAGGGTGTACAGAGGATC
CGAGCCAGGGACATCGTCCCTGGGGACATTGTGGAAGTGGCAGTGGGAGACAAGGTACCCGCTGACCTCC
GTCTCATCGAGATCAAGTCCACCACACTTCGAGTGGACCAGTCCATCCTGACAGGGGAATCCGTGTCTGT
GACGAAGCACACAGATGCCATTCCAGACCCCCGAGCCGTGAACCAGGACAAGAAGAACATGCTATTTTCT
GGCACCAATATTGCATCAGGCAAAGCCCTGGGTGTGGCTGTGGCTACAGGCCTGCAGACAGAGCTGGGCA
AGATTCGGAGCCAGATGGCGGCTGTGGAGCCTGAGCGGACGCCATTGCAGCGCAAGCTGGATGAATTTGG
GAGGCAGCTGTCCCATGCCATCTCTGTGATCTGTGTGGCTGTGTGGGTCATCAACATCGGCCACTTTGCT
GACCCGGCCCACGGTGGATCCTGGCTCCGTGGTGCAGTCTACTACTTCAAGATCGCTGTGGCCCTGGCTG
TGGCTGCTATCCCCGAGGGTCTCCCAGCAGTCATCACTACATGCCTGGCCCTGGGCACAAGACGTATGGC
ACGTAAGAATGCCATTGTGCGGAGCCTGCCTTCTGTGGAGACGCTGGGCTGCACCTCGGTCATCTGCTCT
GACAAGACGGGGACGCTCACCACCAATCAGATGTCTGTCTGCAGGATGTTCGTGGTGGCTGAAGCAGAGG
CAGGCACCTGTCGTTTGCATGAATTCACCATTTCGGGTACCACGTACACCCCAGAGGGCGAAGTGAGGCA
AGGGGAGCAGCCTGTGCGCTGTGGGCAGTTTGATGGGCTAGTGGAGCTCGCCACCATCTGTGCCCTCTGC
AATGATTCTGCGCTGGACTACAATGAGGCCAAGGGTGTGTATGAGAAGGTAGGAGAGGCCACAGAGACCG
CCCTGACTTGCCTGGTAGAGAAGATGAATGTGTTTGACACAGACCTGAAAGGACTGTCGCGGGTGGAGCG
TGCTGGCGCCTGCAACTCAGTCATCAAGCAACTCATGCGGAAAGAGTTCACCCTGGAGTTCTCCCGGGAC
CGGAAGTCTATGTCCGTGTACTGCACACCTACTCGTGCTGACCCCAAGGTCCAGGGCAGCAAGATGTTTG
TGAAGGGAGCTCCTGAGAGTGTAATTGAGCGCTGTAGCTCAGTCCGAGTGGGCAGCCGAACAGCACCCCT
GAGCACCACTTCCAGAGAGCATATCCTGGCCAAGATCCGGGACTGGGGCTCAGGCTCTGACACACTGCGC
TGCTTAGCCTTGGCCACCAGGGACACACCCCCAAGGAAGGAGGACATGCATCTAGATGACTGCAGCCGGT
TTGTACAGTACGAGACAGACCTGACCTTCGTGGGCTGCGTGGGCATGTTAGACCCACCGAGACCAGAGGT
GGCTGCTTGTATCACACGCTGTTCCCGGGCTGGCATTCGAGTGGTCATGATCACAGGGGACAACAAAGGG
ACAGCTGTGGCCATCTGCCGCCGACTTGGCATCTTTGGGGACACGGAGGATGTGTTGGGCAAGGCCTACA
CGGGCCGTGAGTTTGATGACCTCAGCCCAGAGCAGCAGCGCCAGGCTTGTCGCACTGCCCGGTGCTTTGC
CCGAGTGGAACCCGCACATAAGTCTCGAATCGTGGAGAACCTACAGTCCTTTAATGAGATCACTGCCATG
ACTGGCGATGGGGTGAATGATGCACCAGCCCTGAAGAAGGCAGAGATCGGCATCGCCATGGGCTCAGGCA
CTGCTGTGGCCAAGTCAGCAGCAGAGATGGTGCTGTCCGATGACAACTTTGCCTCCATTGTGGCTGCAGT
AGAAGAGGGCAGGGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTTGGC
GAGGTTGTCTGCATCTTCCTCACAGCAATTCTGGGCCTGCCCGAAGCTCTGATCCCTGTGCAGCTGCTCT
GGGTGAACCTGGTAACAGATGGCTTACCGGCCACAGCCCTTGGCTTCAACCCACCAGATCTGGATATCAT
GGAGAAGCCGCCTCGGAATCCCCGTGAGGCCCTCATCAGTGGCTGGCTTTTTTTCCGCTATTTGGCTATT
GGAGTGTATGTAGGCCTGGCTACAGTGGCTGCCGCCACCTGGTGGTTCCTGTATGACACCGAGGGACCTC
AAGTCACCTTCTATCAGCTGAGGAACTTCCTGAAGTGCTCTGAAGACAATCCACTGTTTGCTGGCATTGA
CTGCAAGGTTTTTGAGTCACGCTTCCCCACAACCATGGCCTTATCTGTGCTTGTGACCATTGAAATGTGC
AACGCCCTCAACAGCGTCTCTGAGAACCAGTCACTGCTGCGCATGCCACCCTGGCTGAACCCTTGGCTGC
TGGGGGCTGTGGTCATGTCCATGGCTCTGCATTTTCTTATCCTCCTGGTGCCACCTCTGCCTCTCATTTT
TCAGGTGACCCCACTGAGTGGTCGGCAGTGGGGGGTGGTGCTTCAGATGTCTCTGCCTGTCATCCTGCTC
GATGAGGCCCTCAAGTATCTGTCCAGGAATCACATGGATGGTGTTCTCGGGACATTTATGCAGGCTCGGA
GTAGGCAGCTGCCGACTACTTCCAGGACCCCATACCACACCGGGTTGGCTTCTTGGAAAAAAAGGACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216250 representing NM_001163337
Red=Cloning site Green=Tags(s)

MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAAL
VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI
RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFS
GTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA
DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS
DKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALC
NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRD
RKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLR
CLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKG
TAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM
TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVG
EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAI
GVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMC
NALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILL
DEALKYLSRNHMDGVLGTFMQARSRQLPTTSRTPYHTGLASWKKRT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163337
ORF Size 3078 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163337.1, NP_001156809.1
RefSeq Size 4606 bp
RefSeq ORF 3081 bp
Locus ID 53313
UniProt ID Q64518
Cytogenetics 11 B4
Summary This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001163337" in other vectors (3)
SKU Description Size Price
MR216250 Atp2a3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3 10 ug
$589.00 $893.00
MR216250L3 Lenti ORF clone of Atp2a3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3 10 ug
$1,193.00
MR216250L4 Lenti ORF clone of Atp2a3 (mGFP-tagged) - Mouse ATPase, Ca++ transporting, ubiquitous (Atp2a3), transcript variant 3 10 ug
$1,193.00

file-alt Citations

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