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Klhl6 (NM_183390) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216248
Klhl6 (tGFP-tagged) - Mouse kelch-like 6 (Drosophila) (Klhl6), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $776.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl6
Synonyms MGC118659
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216248 representing NM_183390
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGACAAAGAGGCGCCTGCACCATGAGCGATGTGGTAGAGAGGAGCTTGGAAGGACCCTTAGCAC
TGACTACAGACAAGTCCTCCCAGAAAAGGGGAGACTTGGTGGAAATATTAACTGGGGAGAAGGTAAAATT
TGACGACACGAGACTTTCTTTAATTCTTCAGAACGGCCTGGAGACGCTGAGAGAGGAAAATGCTTTGACA
GACGTCATCTTGTGTGTGGATGTTCAGGAATTCTCTTGCCATCGAGTGGTGCTTGCTGCAGCCAGCAACT
ATTTCAGGGCCATGTTCTGCAACGACTTAAAAGAAAAGTATGAGAAGAGGATCATTATTAAAGGGGTGGA
TGCTGAGACCATGCACACTCTCCTGGTCTACACATACACAAGCAAGGCTCTGATCACCAAGCAGAATGTT
CAGCGGGTTCTGGAGGCTGCCAACCTTTTCCAGTTTTTACAGCTGGTGGATGCCTGTGCCAGCTTTCTCA
CAGAAGCATTGAACCCGGAAAATTGTATTGGAATACTGAGGCTGGCAGACACACATTCTTTGGACAGTTT
AAAGATGCAAGTCCAAAGTTACATCATTCAAAACTTTGTTCAGATTCTGAATTCCGAGGAGTTCCTTGAA
CTTCCTGTGGACACTTTGTACCACATCTTGAGGAGTGACGACCTGTGTGTGACTGAAGAGGCTCAGGTGT
TTGAGACTGTGATGAGCTGGGTCCGGCACAAACAATCAGAACGACTCTGCCTGCTCCCTTGTGTCCTCGA
GAATGTGCGTTTACCACTTCTGGATCCGTGGTACTTCGTGGAGATGGTTGAAGCAGACCCTCTTATTAGA
CAGTGCCCTGAAGTCTTCCCGCTGCTTCAGGAAGCCAGGATGTACCACCTTTCTGGCAATGAGATCATTT
CGGAGCGCACCAAGCCCAGGATGCATGAGTTCCAGTCTGAGGTGTTCATGATTATTGGCGGCTGTACAAA
GGATGAACGATTTGTGGCAGAGGTAACCTGCCTGGACCCCCTGAGGCGCAGTCGCCTGGAGGTGGCCAAG
CTCCCACTGACAGAGCATGAACTGGAGAGTGAGAATAAGAAGTGGGTGGAGTTTGCATGTGTGACATTAA
AAAATGAGGTCTACATATCAGGTGGCAAAGAAACACAGCATGATGTTTGGAAGTACAATTCCTCCATCAA
CAAATGGATTCAGATCGAATATTTAAACATAGGTCGCTGGAGGCATAAGATGGTCGTATTGGGTGGCAAA
GTCTATGTACTTGGGGGTTTTGATGGCTTACAAAGAATCAACAATGTGGAGACATATGACCCCTTCCACA
ACTGCTGGTCAGAGGCTGCACCCCTCCATGTCCATGTCAGTTCTTTTGCAGCTACCAGCCACAAGAAGAA
GTTGTATGTCATTGGAGGAGGACCCAATGGAAAGCTTGCCACAGACAAGACTCAGTGTTATGATCCTTCC
ACCAATAAATGGGTTTTGAAGTCAGCCATGCCAGTAGAGGCTAAATGCATCAATGCAGTGAGTTTCCAGG
ATCACATCTATGTTGTTGGTGGTGCCATGAGAGCACTCTACGCTTATAGCCCTCTGGAAGACAGCTGGTG
TCTGGTGACACAGCTCAGCCATGAGCGAGCCAGCTGTGGCATCGCACCCTGCAACAACAAGCTGTATATC
ACGGGTGGGAGGGACGAGAAGAATGAGGTCATTGCCACTGTGCTCTGCTGGGACCCTGAGGCTCAGAAGC
TGACTGAGGAATGTGTGCTGCCACGAGGAGTATCACATCATGGCAGTGTTACCATCAGGAAGTCATATAC
TCACATCCGGAGGGTTGTCCCTGGAGCAGTGTCTGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216248 representing NM_183390
Red=Cloning site Green=Tags(s)

MAGQRGACTMSDVVERSLEGPLALTTDKSSQKRGDLVEILTGEKVKFDDTRLSLILQNGLETLREENALT
DVILCVDVQEFSCHRVVLAAASNYFRAMFCNDLKEKYEKRIIIKGVDAETMHTLLVYTYTSKALITKQNV
QRVLEAANLFQFLQLVDACASFLTEALNPENCIGILRLADTHSLDSLKMQVQSYIIQNFVQILNSEEFLE
LPVDTLYHILRSDDLCVTEEAQVFETVMSWVRHKQSERLCLLPCVLENVRLPLLDPWYFVEMVEADPLIR
QCPEVFPLLQEARMYHLSGNEIISERTKPRMHEFQSEVFMIIGGCTKDERFVAEVTCLDPLRRSRLEVAK
LPLTEHELESENKKWVEFACVTLKNEVYISGGKETQHDVWKYNSSINKWIQIEYLNIGRWRHKMVVLGGK
VYVLGGFDGLQRINNVETYDPFHNCWSEAAPLHVHVSSFAATSHKKKLYVIGGGPNGKLATDKTQCYDPS
TNKWVLKSAMPVEAKCINAVSFQDHIYVVGGAMRALYAYSPLEDSWCLVTQLSHERASCGIAPCNNKLYI
TGGRDEKNEVIATVLCWDPEAQKLTEECVLPRGVSHHGSVTIRKSYTHIRRVVPGAVSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183390
ORF Size 1857 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183390.3, NP_899246.2
RefSeq Size 2405 bp
RefSeq ORF 1860 bp
Locus ID 239743
UniProt ID Q6V595
Cytogenetics 16 A3
Summary Involved in B-lymphocyte antigen receptor signaling and germinal center formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183390" in other vectors (4)
SKU Description Size Price
MC219768 Klhl6 (untagged) - Mouse kelch-like 6 (Drosophila) (Klhl6), (10ug) 10 ug
$635.00
MR216248 Klhl6 (Myc-DDK-tagged) - Mouse kelch-like 6 (Drosophila) (Klhl6) 10 ug
$289.00 $576.00
MR216248L3 Lenti ORF clone of Klhl6 (Myc-DDK-tagged) - Mouse kelch-like 6 (Drosophila) (Klhl6) 10 ug
$876.00
MR216248L4 Lenti ORF clone of Klhl6 (mGFP-tagged) - Mouse kelch-like 6 (Drosophila) (Klhl6) 10 ug
$876.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.