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Arhgef6 (NM_152801) Mouse Tagged ORF Clone

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SKU
MG216238
Arhgef6 (tGFP-tagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $928.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgef6
Synonyms 1600028C08Rik; 1700038J06Rik; 4930592P22Rik; alpha-PIX
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216238 representing NM_152801
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAATCACGGATGTGGCTTAGTCTTCACAGCTGCCCCGAGAGCTGTTCAGAGCGTCCGAGAGCCTCAG
TCATGAATCCAGAAGAACGCCTTGTGACATGGCTTATATCTTTGGGAGTTCTAGAGTCTCCCAAAAAGAC
TGTCTGTGATCCAGAGGAGTTCTTGAAGTCCTCGCTGAAAAATGGGGTAGTGTTGTGCAAACTCATAAAC
AGACTTCTGCCTGGCTCGGTGGAGAAGTATTGTCTGGAACCCCAAACTGAGGCCGACTGCATCGACAACA
TCAATGACTTCCTGAAAGGATGTGCAACTCTTCAAGTGGAAGTGTTTGAGCCTGATGACCTCTATTCGGG
GGCCAATTTCTCCAAGGTTCTGAATACTCTTTTAGCTGTCAACAAAGCAACTGAAGATCAGCTCTCAGAA
AGACCATGTGGAAGGTCCTCTTCTCTTAGTGCTGCGACCAGCTCTCAGACAAACCCGCAGGTGGCCGTTC
CCAGCACAGCTCCAGAGCAGCACAGCGAGGAAAAGGCAGAGATGACGGAAAATGGAAGTCACCAGTTGAT
AGTAAAGGCAAGATTCAACTTCAAGCAGACGAATGAAGATGAATTGTCAGTGTGTAAAGGGGACATCATT
TATGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACTGGATGGTTCCCTA
GTAATTATGTCCGAGAAATTAAACCCAGTGAGAGACCTCTGTCCCCTAAGGCTATCAAAGGATTTGACAC
TGCTCCACTTACCAAGAATTACTACACTGTGGTGTTACAGAACATCTTGGACACTGAAAAAGAATATGCC
AAAGAACTTCAGTCTCTTCTTGTTACTTACCTAAGACCCTTGCAGTCTAATAACAACCTGAGTACTGTGG
AGTTTACATGTTTATTGGGAAACTTTGAAGAAGTATGCACATTTCAACAGACACTCTGCCAAGCCTTGGA
AGAATGTTCAAAGTTTCCAGAAAACCAACACAAAGTAGGAGGCTGCCTACTGAACCTCATGCCTCATTTC
AAATCGATGTACCTGGCTTACTGTGCAAATCATCCATCAGCTGTCAACGTGCTCACCCAGCACAGTGATG
ATCTAGAACGATTCATGGAAAATCAAGGTGCATCCAGCCCGGGTATCCTCATTCTAACAACAAGTCTCAG
CAAACCATTCATGCGACTGGAAAAGTATGTTACTCTCTTGCAAGAACTGGAACGACATATGGAGGACACT
CATCCAGACCATCAGGATATTCTGAAAGCAATCATAGCATTCAAAACACTCATGGGGCAGTGTCAAGATC
TGAGAAAGAGAAAGCAGCTGGAATTGCAGATCCTTTCAGAACCTATTCAGGCATGGGAAGGAGATGATAT
TAAGACTTTGGGAAATGTGATCTTTATGTCTCAAGTAGTGATGCAGCATGGGGCCTGTGAGGAGAAAGAG
GAACGATATTTTCTGCTGTTTTCAAGTGTCTTGATAATGTTGTCTGCAAGTCCTCGGATGAGTGGCTTTA
TGTATCAGGGAAAAATACCAATAGCAGGGATGGTAGTGAACAGATTGGATGAAATTGAAGGCAGTGACTG
TATGTTCGAAATCACAGGTAGCACGGTTGAGAGAATTGTGGTCCATTGCAACAATAACCAGGACTTCCAG
GAGTGGATGGAGCAGCTGAACAGACTGACCAAAGGACCAACCTCTTGCGGTTCATTGTCTAAGACCTCCT
CCTCATCATGTAGTACTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAGCCTCCTCA
AATTATAAAACCGTGGAGTTTAAGTTGCCTACGACCTGCACCTCCACTTAGACCATCAGCAGCACTAGGT
TATAAAGAGAGGATGTCTTATATCTTAAAGGAATCTAGTAAGAGCCCCAAAACAATGAAGAAGTTTCTTC
ACAAAAGAAAGACGGAGAGGAAAGCATCCGAGGAAGAATACGTGATTAGGAAAAGTACTGCTGCCCTGGA
AGAGGATGCTCAGATTCTTAAGGTGATCGAAGCTTATTGCACCAGCGCCAGTTTTCAACAAGGTACTCGT
AAAGATTCTGTTCCACAAGTTCTACTCCCCGAGGAAGAGAAACTCATTATTGAAGAAACCAGAAGTAATG
GGCAGACCATCATTGAAGAGAAGAGCCTTGTTGATACTGTCTATGCCTTGAAGGATGAGGTCAAGGAACT
GAAGCAGGAGAATAAAAAAATGAAGCAATGTCTGGAAGAAGAATTGAAGTCGAGGAAGGACCTAGAGAAG
CTGGTCCGGAAGCTGTTGAAACAAACAGATGAATGCATTCGATCTGAATCCAGCAGCAAGACCTCGATTC
TCCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216238 representing NM_152801
Red=Cloning site Green=Tags(s)

MQSRMWLSLHSCPESCSERPRASVMNPEERLVTWLISLGVLESPKKTVCDPEEFLKSSLKNGVVLCKLIN
RLLPGSVEKYCLEPQTEADCIDNINDFLKGCATLQVEVFEPDDLYSGANFSKVLNTLLAVNKATEDQLSE
RPCGRSSSLSAATSSQTNPQVAVPSTAPEQHSEEKAEMTENGSHQLIVKARFNFKQTNEDELSVCKGDII
YVTRVEEGGWWEGTLNGRTGWFPSNYVREIKPSERPLSPKAIKGFDTAPLTKNYYTVVLQNILDTEKEYA
KELQSLLVTYLRPLQSNNNLSTVEFTCLLGNFEEVCTFQQTLCQALEECSKFPENQHKVGGCLLNLMPHF
KSMYLAYCANHPSAVNVLTQHSDDLERFMENQGASSPGILILTTSLSKPFMRLEKYVTLLQELERHMEDT
HPDHQDILKAIIAFKTLMGQCQDLRKRKQLELQILSEPIQAWEGDDIKTLGNVIFMSQVVMQHGACEEKE
ERYFLLFSSVLIMLSASPRMSGFMYQGKIPIAGMVVNRLDEIEGSDCMFEITGSTVERIVVHCNNNQDFQ
EWMEQLNRLTKGPTSCGSLSKTSSSSCSTHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLRPSAALG
YKERMSYILKESSKSPKTMKKFLHKRKTERKASEEEYVIRKSTAALEEDAQILKVIEAYCTSASFQQGTR
KDSVPQVLLPEEEKLIIEETRSNGQTIIEEKSLVDTVYALKDEVKELKQENKKMKQCLEEELKSRKDLEK
LVRKLLKQTDECIRSESSSKTSILQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152801
ORF Size 2385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152801.2, NP_690014.2
RefSeq Size 4580 bp
RefSeq ORF 2388 bp
Locus ID 73341
UniProt ID Q8K4I3
Cytogenetics X A6
Summary Acts as a RAC1 guanine nucleotide exchange factor (GEF).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_152801" in other vectors (4)
SKU Description Size Price
MC221714 Arhgef6 (untagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6), (10ug) 10 ug
$802.00
MR216238 Arhgef6 (Myc-DDK-tagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6) 10 ug
$589.00 $728.00
MR216238L3 Lenti ORF clone of Arhgef6 (Myc-DDK-tagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6) 10 ug
$1,028.00
MR216238L4 Lenti ORF clone of Arhgef6 (mGFP-tagged) - Mouse Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (Arhgef6) 10 ug
$1,028.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.