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Pdcd4 (NM_001168492) Mouse Tagged ORF Clone

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SKU
MG216227
Pdcd4 (tGFP-tagged) - Mouse programmed cell death 4 (Pdcd4) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pdcd4 or NM_001168492"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdcd4
Synonyms D19Ucla1; Ma3; Tis
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216227 representing NM_001168492
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATATAGAAAATGAGCAGACACTGAATGTGAACCCCACTGACCCTGACAATTTAAGCGACTCTCTCT
TTTCTGGAGATGAAGAAAATGCTGGCACTGAAGAAATAAAGAATGAAATAAATGGAAATTGGATTTCCGC
ATCTACTATTAATGAAGCTAGAATCAATGCAAAAGCCAAAAGACGACTGCGGAAAAATTCATCCCGGGAC
TCTGGCCGAGGAGACTCAGTCAGTGACAATGGAAGTGAAGCGGTTAGAAGTGGAGTTGCTGTGCCCACCA
GTCCAAAAGGAAGGTTGCTAGATAGGCGGTCCAGATCTGGGAAAGGAAGGGGGCTGCCAAAGAAAGGTGG
TGCAGGCGGCAAGGGTGTCTGGGGCACACCTGGACAGGTGTATGATGTGGAAGAGGTGGATGTGAAAGAT
CCAAACTATGATGACGACCAGGAGAACTGTGTTTATGAAACTGTAGTTTTGCCCCTGGATGAGACCGCAT
TTGAGAAGACTCTAACACCAATTATACAGGAATACTTTGAGCATGGAGATACAAATGAAGTTGCGGAGAT
GTTAAGAGACTTAAACCTTGGGGAGATGAAGAGTGGCGTGCCGGTGTTGGCAGTGTCCTTAGCCTTGGAG
GGGAAGGCCAGCCACCGGGAGATGACATCCAAGCTGCTTTCTGACCTTTGCGGGACGGTGATGAGCACAA
ATGACGTGGAAAAGTCATTTGACAAGTTGCTGAAGGATCTCCCTGAGCTAGCCTTGGACACTCCTAGGGC
ACCGCAGTTGGTGGGCCAGTTTATTGCTAGAGCTGTTGGAGATGGAATCTTATGTAATACCTATATCGAT
AGTTACAAAGGAACTGTAGATTGTGTACAGGCTCGAGCTGCTCTGGATAAGGCTACTGTGCTCCTGAGTA
TGTCCAAAGGCGGGAAGCGGAAAGACAGTGTGTGGGGATCTGGAGGCGGGCAACAGCCTGTCAATCACCT
TGTTAAAGAGATTGATATGCTGCTTAAAGAGTATTTACTCTCTGGAGATATATCTGAAGCTGAACACTGC
CTTAAGGAACTGGAAGTACCTCATTTTCACCACGAGCTTGTATATGAAGCCATTGTAATGGTTTTAGAGT
CAACTGGAGAAAGTGCATTCAAGATGATCTTAGATTTATTAAAATCCTTGTGGAAGTCTTCTACTATTAC
CATAGACCAAATGAAAAGAGGCTATGAGAGAATTTACAATGAAATCCCAGACATTAATCTGGATGTCCCA
CACTCATACTCTGTTCTTGAGAGATTTGTGGAGGAATGTTTTCAGGCTGGAATAATTTCCAAACAACTCC
GTGATCTTTGTCCATCAAGGGGAAGAAAGCGTTTTGTAAGTGAAGGAGATGGAGGCCGTCTTAAACCTGA
GAGCTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216227 representing NM_001168492
Red=Cloning site Green=Tags(s)

MDIENEQTLNVNPTDPDNLSDSLFSGDEENAGTEEIKNEINGNWISASTINEARINAKAKRRLRKNSSRD
SGRGDSVSDNGSEAVRSGVAVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKD
PNYDDDQENCVYETVVLPLDETAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALE
GKASHREMTSKLLSDLCGTVMSTNDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYID
SYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQPVNHLVKEIDMLLKEYLLSGDISEAEHC
LKELEVPHFHHELVYEAIVMVLESTGESAFKMILDLLKSLWKSSTITIDQMKRGYERIYNEIPDINLDVP
HSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001168492
ORF Size 1407 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001168492.1, NP_001161964.1
RefSeq Size 1806 bp
RefSeq ORF 1410 bp
Locus ID 18569
UniProt ID Q61823
Cytogenetics 19 48.73 cM
Summary Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001168492" in other vectors (4)
SKU Description Size Price
MC216276 Pdcd4 (untagged) - Mouse programmed cell death 4 (Pdcd4), transcript variant 3, (10ug) 10 ug
$503.00
MR216227 Pdcd4 (Myc-DDK-tagged) - Mouse programmed cell death 4 (Pdcd4), transcript variant 3 10 ug
$289.00 $457.00
MR216227L3 Lenti ORF clone of Pdcd4 (Myc-DDK-tagged) - Mouse programmed cell death 4 (Pdcd4), transcript variant 3 10 ug
$757.00
MR216227L4 Lenti ORF clone of Pdcd4 (mGFP-tagged) - Mouse programmed cell death 4 (Pdcd4), transcript variant 3 10 ug
$757.00

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