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Adam29 (NM_175939) Mouse Tagged ORF Clone

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SKU
MG216220
Adam29 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 29 (Adam29), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $899.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adam29
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216220 representing NM_175939
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATATGATTGAAGCATTATTATCCATGAGAGTCTTGTTCCTGACACAAGTGTTTGGGATTTTCCTGT
GTTTTCCTGGACTCACAAAGGCTGGACATCTGCACTACCACAGTTCCATAGAAGTGGTGATTCCCATGAA
GGTAACTGAGAAAACCAGAGGAATGAACCTTCCAAATTGGATCTCCTATAGCCTTAAACTTGGAGGCCAG
AGATACATCATCCACATGAAGATCAAGAATCTTTTTCTAACCAGGCACCTTCCAGTGTTCACCTACTCTG
ATCAGGACTCTCTGCTTGAAGATTACCCTTTTGTACAGGATGACTGCTACTACCAAGGTTATGTGGAGGG
TGACTCAGAATCATTAGTTTCCCTCAGTTCCTGTTTTGGAGGCTTTCATGGACTATTAGAGATAAATAAT
ATTGTTTATGAAATTATGCCCAAGAAGTTTTCTAGGAAATTTGAACATCTGGTCTATAAAGTGGACATTA
ATAAAACAGAATCAAGGGGTTCCAGCCTTATGCAAGATAACATAACATGCCAAGTAGAGTTACAAAAAAG
TGGTAATCCCATTCTCAAGCAAAGTAGTTTTGAAGACTGGTGGACCCATACTAAAATTGTTGAATTAGTA
GTGGTGGTGGATAAGACTCTATATGACCACTATGGAAATTATACAGTAATGCTGTCAGATCTGTATTCTG
TGATAAATATAGTGGATACCATTTATGAGGTAATTGGTATTAAAATATTATTGGTTGGTGTGGAGGTTTG
GAATAAGAAAAATCTTATTGTGATAGATGACGTAAGTAAATCTCTAAGACTATATTGCCGGTGGAAAGCC
TCAAACTTTCTTCATCGTTTAAAACATGATGTCTCGCATCTTTTCATATATAGGCACTTGAGAGGATTAA
GTGGCATAGGTTCCACTGGGGGGATTTGTGATCCAAAACGTAGTTGTGCAGTTGTTACTTTCATAGACAG
AACTTTGAACCTTCGTGCCATTGGAGTGGCTCATCACTTAGGTCATAATTTGGGCATGAAACATGATGAA
GATATATGTAAGTGTAGTTACAGTAAATGTATAATGCACATGGACAGCCCACCGATACCCAAATTCAGCA
ATTGTAGCTATAATTACTTTTGGTCTTACACTGTAAAGAACACAAGGTGTTTGATGGAAAACATGTACAC
AAAGGATATCTTTGACAGGACACGCTGTGGAAATGGTGTTGTTGAAGACAAAGAACAATGTGACTGTGGA
TCATTAAGGAATTGTACAAATGACCTTTGTTGCATGTCAAACTGCACTCTGAGTACTGGGTCTTCCTGTG
CCTTTGGACTTTGCTGCAAAAACTGTCAGTTTTTACCATCAGGGACTCTGTGTAGAAAAAGGGATAACAT
TTGTGACCTTCCAGAGTGGTGCAATGGGACGTCCCATGAATGTCCAGATGATGCTTATGTAGAAGATGGA
ATTCCCTGTGGGGTCTCAGCCTATTGCTATGAAAAGCAATGTAATGACCGCAATGAGCACTGTAGGCAAA
TTTTTGGCCAGAATGCAAAGACTGCAAGTGTACATTGCTACAGAGAAATAAACACTAAAGGTGATCGTTT
TGGCCATTGTGGTCTTCAGGGACCTACTTACATAAAATGTAAAAGCAATGATGCTCTTTGTGGAAGAATT
CAATGTGATAATGTGGTACAAATTCCCAATATGAAAGATCACAGTACTATTCACTTTGCTCTTGTCAAAA
ATGTATCTTGCTGGGGCACTGATTACCACACTGGGACAAGCCTAACTGATATAGGCGATGTGAAAGATGG
CACAGAGTGTGAGCAAAATCATATCTGTATCAATAGGCATTGTGTACATATATCTACATTAGACAGCAAC
TGTACACCTGCATTCTGTAATTACAGGGGCATCTGTAACAATAAACATCACTGCCACTGCAACTTCCACT
GGGATCCTCCTAACTGTATGATTAGAGGACATGGAGGTAGTGTAGACAGTGGCTTACCTCCTAAAACAAA
TAAAAAGAAACATTTCTTCTATCTGCTTCTATTACAGCTCATTATTTTGGCTTGCCTTTTAAGTTGTCTT
CTTTGGCTACTTTTTAATATAAAAGGAAGTAAACGAAAGCCCCAAGTTCAGCCTACACCTGTAAAAACAA
AGAAAGTTTCAAAGAAAGTTCCAAGCCAAAAACCGAGTCCAGTGCCTTCCCCGAGTCTACCTCAATTAAG
AATGCCATCACGATCTGCTTCACCAACATCATCCATAAAAAGTACCAAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216220 representing NM_175939
Red=Cloning site Green=Tags(s)

MNMIEALLSMRVLFLTQVFGIFLCFPGLTKAGHLHYHSSIEVVIPMKVTEKTRGMNLPNWISYSLKLGGQ
RYIIHMKIKNLFLTRHLPVFTYSDQDSLLEDYPFVQDDCYYQGYVEGDSESLVSLSSCFGGFHGLLEINN
IVYEIMPKKFSRKFEHLVYKVDINKTESRGSSLMQDNITCQVELQKSGNPILKQSSFEDWWTHTKIVELV
VVVDKTLYDHYGNYTVMLSDLYSVINIVDTIYEVIGIKILLVGVEVWNKKNLIVIDDVSKSLRLYCRWKA
SNFLHRLKHDVSHLFIYRHLRGLSGIGSTGGICDPKRSCAVVTFIDRTLNLRAIGVAHHLGHNLGMKHDE
DICKCSYSKCIMHMDSPPIPKFSNCSYNYFWSYTVKNTRCLMENMYTKDIFDRTRCGNGVVEDKEQCDCG
SLRNCTNDLCCMSNCTLSTGSSCAFGLCCKNCQFLPSGTLCRKRDNICDLPEWCNGTSHECPDDAYVEDG
IPCGVSAYCYEKQCNDRNEHCRQIFGQNAKTASVHCYREINTKGDRFGHCGLQGPTYIKCKSNDALCGRI
QCDNVVQIPNMKDHSTIHFALVKNVSCWGTDYHTGTSLTDIGDVKDGTECEQNHICINRHCVHISTLDSN
CTPAFCNYRGICNNKHHCHCNFHWDPPNCMIRGHGGSVDSGLPPKTNKKKHFFYLLLLQLIILACLLSCL
LWLLFNIKGSKRKPQVQPTPVKTKKVSKKVPSQKPSPVPSPSLPQLRMPSRSASPTSSIKSTN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175939
ORF Size 2289 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175939.2
RefSeq Size 2742 bp
RefSeq ORF 2292 bp
Locus ID 244486
Cytogenetics 8 B2
Summary This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. provided by RefSeq, May 2016
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Documents
Resources
"NM_175939" in other vectors (4)
SKU Description Size Price
MC221465 Adam29 (untagged) - Mouse a disintegrin and metallopeptidase domain 29 (Adam29), (10ug) 10 ug
$769.00
MR216220 Adam29 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 29 (Adam29) 10 ug
$589.00 $699.00
MR216220L3 Lenti ORF clone of Adam29 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 29 (Adam29) 10 ug
$999.00
MR216220L4 Lenti ORF clone of Adam29 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 29 (Adam29) 10 ug
$999.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.