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Tnrc6b (NM_144812) Mouse Tagged ORF Clone

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SKU
MG216215
Tnrc6b (tGFP-tagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,721.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tnrc6b or NM_144812"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tnrc6b
Synonyms 2700090M07Rik; A730065C02Rik; AI848765; D230019K20Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216215 representing NM_144812
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAACCAATGAGGGAGAAGTAGAGGAAGAAAGCAGCTCCCAGGTGGAACAGGAGGATTTTGTAATGG
AAGGGCATGGCAAGACTCCACCTCCTGGAGAAGAAAGCAAACAAGAGAAGGAGCAAGAAAGGGAAGAACA
GTTAATGGAAGACAAGAAAAGGAAGAAAGAGGATAAAAAGAAAAAAGAAGCCACCCAGAAGGTCACGGAA
CAAAAAACCAAAGTGCCCGAAGTGACGAAACCAAGTTTAAGCCAACCAACGGCCGCCAGCCCAATTGGCA
GCTCTCCATCGCCACCAGTCAATGGTGGCAACAATGCCAAAAGGGTGGCAGTGCCGAACGGACAACCGCC
AAGCGCCGCCCGCTACATGCCTCGGGAGGTGCCACCGCGATTCCGTTGCCAGCAGGACCATAAAGTGTTA
CTAAAACGTGGGCAGCCCCCTCCACCGTCCTGCATGCTCCTCGGGGGTGGGGCGGGGCCTCCTCCCTGCA
CAGCACCTGGAGCAAACCCCAACAACAACGCACAAGTGACAGGAGCGCTGCTGCAGAGTGAGAGTGGGAC
TGCACCAGAGTCAACCCTTGGAGGTGCTGCTGCTTCAAATTATGCAAATTCCACTTGGGGCCCGGGAGCC
TCCTCCAACAGCGGCGCCTCCCCCAACCCAATTCACATCTGGGACAAGGTGATTGTAGACGGGTCTGACA
TGGAAGAGTGGCCTTGTATTGCCAGCAAAGACACTGAATCTTCTTCCGAAAACACCACCGATAACAACAG
TGCCTCGAACCCTGGCTCTGAGAAGAGCAGTCTGCCAGGAAGCACCACTAGTAACAAAGGCAAAGGGAGC
CAATGCCAGGCTGCAAGTTCTGGGAACGAATGTAATCTTGGGGTCTGGAAATCTGACCCCAAGGCTAAGT
CTGTTCAACCTCCCAACTCTACTTCAGATAGCAACAATGGACTAGGAAACTGGAGGAGCACGAGTGGTCA
GGATAGAATTGGACCTGGCTCTGGCTTCAGCAACTTTAACCCAAATAGCAACCCATCTGCCTGGCCAGCA
TTGGTCCAAGAAGGGACTTCTAGAAAAGGGGCATTGGAAACAGAAAGTAGTAGTTCCAGTGCACAAGTTA
GCACAGTAGGTCAGGCATCCAGGGAACAGCAGTCAAAGATGGAAAATGCGGGTGTTAATTTTGTTGTCTC
TGGCAGAGAACAGGCTCAAATTCATAACACTGATGGACCAAAAAATGGAAACACTAACTCCTTGAACTTA
AGTTCACCAAACCCCATGGAAAATAAGGGAATGCCCTTTGGAATGGGCTTGGGGAACACCTCCAGGAGCA
CTGATGCCCCTTCACAAAGCACTGGAGATCGAAAGACTGGGAGTGTTGGGTCTTGGGGTGCAGCTAGGGG
GCCTTCTGGAACTGACACAGTCTCTGGACAAAGCAATTCTGGAAATAATGGGAACAATGGAAAAGACAGA
GAGGACTCCTGGAAAGGAGCTTCTGTTCCAAAACCAACTGGGTCAAAAAGTGACTCTTGGGACAACAATA
ACAGGTCTACGGGTGGGTCCTGGAACTTTGGCCCTCAGGACAATAATGACAACAAATGGGGTGAAGGGAA
CAAAATGACATCTGGGGTCTCTCAGGGAGAATGGAAACAGCCGACTGGGTCTGATGAGTTGAAAATTGGA
GAATGGAGTGGTCCAAACCAACCAAATTCTAGCACTGGAGCATGGGACAATCAAAAGGGCCACCCCCTCC
CTGAAAACCAAGGCAATGCCCAGGCTCCCTGTTGGGGAAGATCTTCCAGCTCCGCAGGAAGTGAAGTTGG
AGGTCAAAGCACTGGAAGCAACCACAAAGCAGGAAGTAGTGACAGTCATAATTCTGGTCGTCGGTCATAT
AGGCCCACGCATCCTGATTGCCAGGCTGTCTTGCAGACTCTTTTGAGCCGGACTGATTTGGACCCTAGGG
TGCTCTCAAACACTGGCTGGGGCCAAACTCAAATTAAGCAGGACACAGTGTGGGATATTGAAGAGGTGCC
AAGGCCTGAGGGGAAATCTGACAAAGGAACTGAGGGGTGGGAGAGCGCTGCCACACAGACCAAGAACTCA
GGGGGCTGGGGAGATGCACCCAGCCAAAGCAATCAAATGAAGTCTGGATGGGGGGAGCTCTCAGCCTCGA
CAGAGTGGAAAGACCCCAAGAGCACAGGAGGCTGGAATGACTATAAGAACAACAACTCTTCTAACTGGGG
AGGAGGCCGAGCAGATGAAAAGACACCTTCCTCTTGGAATGAGAGTTCTTGTAAGGATCAGGGGTGGGGA
GGTGGGCGCCAACCCAACCAAGGATGGACTTCTGGGAAGAATGGTTGGGGCGAGGAGGTGGATCAAGTGA
AAAATAATAATTGGGAAAGTTCTGCCAATAAGCCTGTGTCTGGGTGGGGTGAAGGAGGACAGAATGAAAT
TGGAACTTGGGGTAATGGTGGGAATACAAACCTAGCATCAAAAGGTGGGTGGGAAGATTGCAAAAGATCC
CCTGCATGGAATGAGACAGGCAGACAGCCCAATTCCTGGAATAAACAACACCAACAGCAGCAGCAGCCAC
CGCCACCACAGCCAGAGGCTTCTGGTTCATGGGGAGGCCCACCCCCGCCACCTCAAGGCAATGTTCGACC
TTCTAATTCCAACTGGAGCAGTGGACCCCAGCCTACAACCCCTAAGGATGATGAACCCAGTGGTTGGGAG
GAGCCATCCCCACAGTCAATTAGTCGGAAAATGGACATTGATGATGGCACTTCAGCTTGGGGAGACCCTA
ACAGTTATAACTACAAGAATGTGAACCTTTGGGATAAGAATTCTCAAGGGGGCCCAGCACCTCGAGAACC
AAACCTGCCTACCCCAATGACCGGGAAATCAGCATCAGTCTGGAGCAAAAGCACACCACCTGCTCCAGAT
AATGGGACTTCAGCCTGGGGTGAGCCAAATGAAAGCAGTCCTGGGTGGGGTGAAATGGATGATGCAGGAG
CATCAACCACAGGCTGGGGAAACACACCTGCCAACGCTCCCAATGCCATGAAACCCAATTCCAAATCTAT
GCAAGACGGCTGGGGGGAGAGTGACGGACCAGTCACAGGAGCTCGCCATCCTAGCTGGGAAGAGGAGGAT
GACGGAGGAGTCTGGAACACCGCCGGCTCTCAGGGAAGCACTTCCTCCCACAACTCAGCAAGCTGGGGAC
AAGGAGGAAAGAAACAAATGAAGTGCTCACTAAAAGGAGGAAACAATGATTCATGGATGAATCCTCTTGC
CAAACAGTTTTCAAATATGGGATTGCTGAGTCAAACTGAGGATAACCCAAGCAGCAAAATGGATTTGTCT
GTAGATAAGAAATTTGATGTGGACAAGAGAACAATGAATCTCGGGGATTTTAATGATATCATGAGGAAGG
ATCGACCTGGGTTCCGTCCACCTAATTCCAAAGACTTGGGAACCACAGACAGTGGGCCTTATTTTGAGAA
GGGCGGCAGTCATGGTTTGTTTGGAAACAGCACAGCACAATCGAGAGGCCTGCACACTCCAGTGCAGCCA
CTAAGTTCTTCTCCTGGTCTCCGGGCGCAAGTGCCTCCCCAGTTTATCTCCCCCCAGGTTTCTGCCTCAA
TGCTCAAGCAGTTCCCCAACAGTGGCCTGAATCCAGGTCTCTTTAATGTGGGGCCCCAGCTATCTCCTCA
ACAGATTGCCATGCTGAGCCAGCTTCCACAGATACCCCAGTTTCAGTTGGCATGTCAGCTTCTCCTGCAG
CAGCAGCAGCAACAGCAGCAGTTGCTACAGAACCAGAGAAAGATTTCTCAAGCAGTGCGCCAGCAGCAAG
AACAGCAGCTGGCAAGAATGGTGAGTGCTCTCCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGAGGCA
GCCTAGCATGAAGCATTCACCTTCTCATCCTGTTGGGCCTAAGCCACATTTGGACAACATGGTACCCAAT
GCGCTGAATGTGGGGCTCCCAGACCTTCCAACCAAAGGGCCAATTCCTGGATATGGTTCTGGCTTCAGCT
CTGGTGGCATGGACTATGGTATGGTTGGTGGCAAGGAGGCTGGGACCGAGTCTCGCTTCAAACAGTGGAC
CTCAATGATGGAAGGGCTGCCATCTGTGGCCACACAAGAAGCCACCATGCACAAAAACGGCGCTATAGTG
GCCCCTGGTAAGACCCGAGGAGGTTCACCATACAACCAGTTTGATATAATCCCAGGTGACACACTGGGTG
GCCATACGGGTCCTGCTGGTGATAGCTGGTTACCTGCCAAATCTCCACCGACTAATAAAATCGGAAGTAA
ATCCAGCAATGCCAGTTGGCCTCCAGAATTCCAACCAGGAGTGCCATGGAAAGGTATCCAAAATATTGAC
CCTGAATCTGACCCCTATGTCACCCCAGGAAGTGTGCTAGGGGGTACAACCACATCTCCCATTGTAGATA
CTGACCACCAACTGCTGCGGGATAACACCACAGGGTCTAATTCCTCCCTCAACACCTCGCTGCCTTCACC
TGGTGCCTGGCCCTACAGTGCCTCTGACAACTCCTTTACCAACGTTCATAGCACTTCAGCAAAGTTCCCT
GATTATAAATCCACGTGGTCCCCAGATCCCATAGGACACAACCCCACTCATCTCTCCAATAAGATGTGGA
AAAATCACATCTCCTCCAGGAACACTACACCGCTGACCCGCCCACCTCCTGGCCTGACCAACCCCAAACC
GGCATCTCCTTGGAGCAGCACAGCACCGCGATCGGTCAGGGGGTGGGGGACACAGGACTCCCGGATTGCC
TCGGCCTCTACCTGGAGTGATGGTGGCTCTGTCCGTCCTAGTTACTGGCTGGTTCTCCACAATCTCACCC
CACAGATTGATGGGTCAACCCTGAGAACGATCTGCATGCAGCATGGCCCACTGCTGACATTCCATCTGAA
CCTAACCCAGGGCACTGCCCTGATCCGATACAGCACCAAACAAGAGGCAGCCAAGGCCCAGACTGCACTG
CACATGTGTGTGTTGGGAAACACTACCATCCTGGCCGAGTTTGCCACTGAGGATGAAGTCAGCCGCTTTC
TGGCTCAAGCTCAGCCCCCTACGCCTGCAGCCACCCCGAGTGCACCTGCCACAGGGTGGCAGTCCCTGGA
GACCAGCCAGAACCAAGCAGACCCCGTTGGACCTGCGCTGAACCTTTTCGGTGGGTCCACCGGGCTCGGG
CAGTGGAGCAGCAGCGCTGGTGGCAGCAGTGGGGCCGATCTTGCCGGCACTTCCTTGTGGGGCCCCCCAA
ACTATTCTTCTAGCTTGTGGGGAGTCCCGACGGTGGAAGATCCCCATAGGATGGGCAGCCCTGCTCCTTT
ACTACCTGGTGACCTTCTGGGAGGAGGGTCGGATTCAATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216215 representing NM_144812
Red=Cloning site Green=Tags(s)

MQTNEGEVEEESSSQVEQEDFVMEGHGKTPPPGEESKQEKEQEREEQLMEDKKRKKEDKKKKEATQKVTE
QKTKVPEVTKPSLSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVL
LKRGQPPPPSCMLLGGGAGPPPCTAPGANPNNNAQVTGALLQSESGTAPESTLGGAAASNYANSTWGPGA
SSNSGASPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASNPGSEKSSLPGSTTSNKGKGS
QCQAASSGNECNLGVWKSDPKAKSVQPPNSTSDSNNGLGNWRSTSGQDRIGPGSGFSNFNPNSNPSAWPA
LVQEGTSRKGALETESSSSSAQVSTVGQASREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNL
SSPNPMENKGMPFGMGLGNTSRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTVSGQSNSGNNGNNGKDR
EDSWKGASVPKPTGSKSDSWDNNNRSTGGSWNFGPQDNNDNKWGEGNKMTSGVSQGEWKQPTGSDELKIG
EWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSAGSEVGGQSTGSNHKAGSSDSHNSGRRSY
RPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDIEEVPRPEGKSDKGTEGWESAATQTKNS
GGWGDAPSQSNQMKSGWGELSASTEWKDPKSTGGWNDYKNNNSSNWGGGRADEKTPSSWNESSCKDQGWG
GGRQPNQGWTSGKNGWGEEVDQVKNNNWESSANKPVSGWGEGGQNEIGTWGNGGNTNLASKGGWEDCKRS
PAWNETGRQPNSWNKQHQQQQQPPPPQPEASGSWGGPPPPPQGNVRPSNSNWSSGPQPTTPKDDEPSGWE
EPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTGKSASVWSKSTPPAPD
NGTSAWGEPNESSPGWGEMDDAGASTTGWGNTPANAPNAMKPNSKSMQDGWGESDGPVTGARHPSWEEED
DGGVWNTAGSQGSTSSHNSASWGQGGKKQMKCSLKGGNNDSWMNPLAKQFSNMGLLSQTEDNPSSKMDLS
VDKKFDVDKRTMNLGDFNDIMRKDRPGFRPPNSKDLGTTDSGPYFEKGGSHGLFGNSTAQSRGLHTPVQP
LSSSPGLRAQVPPQFISPQVSASMLKQFPNSGLNPGLFNVGPQLSPQQIAMLSQLPQIPQFQLACQLLLQ
QQQQQQQLLQNQRKISQAVRQQQEQQLARMVSALQQQQQQQQQQQRQPSMKHSPSHPVGPKPHLDNMVPN
ALNVGLPDLPTKGPIPGYGSGFSSGGMDYGMVGGKEAGTESRFKQWTSMMEGLPSVATQEATMHKNGAIV
APGKTRGGSPYNQFDIIPGDTLGGHTGPAGDSWLPAKSPPTNKIGSKSSNASWPPEFQPGVPWKGIQNID
PESDPYVTPGSVLGGTTTSPIVDTDHQLLRDNTTGSNSSLNTSLPSPGAWPYSASDNSFTNVHSTSAKFP
DYKSTWSPDPIGHNPTHLSNKMWKNHISSRNTTPLTRPPPGLTNPKPASPWSSTAPRSVRGWGTQDSRIA
SASTWSDGGSVRPSYWLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTAL
HMCVLGNTTILAEFATEDEVSRFLAQAQPPTPAATPSAPATGWQSLETSQNQADPVGPALNLFGGSTGLG
QWSSSAGGSSGADLAGTSLWGPPNYSSSLWGVPTVEDPHRMGSPAPLLPGDLLGGGSDSI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144812
ORF Size 5430 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144812.3
RefSeq Size 17334 bp
RefSeq ORF 5433 bp
Locus ID 213988
UniProt ID Q8BKI2
Cytogenetics 15 E1
Summary Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144812" in other vectors (1)
SKU Description Size Price
MR216215 Tnrc6b (Myc-DDK-tagged) - Mouse trinucleotide repeat containing 6b (Tnrc6b), transcript variant 1 10 ug
$1,521.00

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