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Syt15 (NM_181529) Mouse Tagged ORF Clone

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SKU
MG216214
Syt15 (tGFP-tagged) - Mouse synaptotagmin XV (Syt15) transcript variant a, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Syt15
Synonyms CHR10SYT; E230025K04Rik; sytXV
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216214 representing NM_181529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGCAGCTGGCCTTCCTGATAGGAGGCATCATTGGAGGGCTGCTTCTGCTGATCGGAGTGAGCT
GCTGTCTGTGGAGGAGGTTTTGCGCCACCTTCACCTATGAGGAACTGCCAGAGACGTCAGACCCGGCCAC
CATCTCTTACTTCAGCAGAAAAGAGGACAGGCTCTACCAGTATTCTGGGACCCCACCTGGCAGGCTACCA
AGTGTACCATTTGTGGTACCCCCTTCTCACCAAGGTCGAGACTGGGTGCCCCTACATGGTGGTGATTGGG
CTGTGGCCCCACAGGACCCCTGCCCTGTCCCAGAGCACATGGCCTGCACCTCCAGTGCCAAACCTGGAGA
TGCATGTGAGATGGGGAGCATCAACCCAGAGCTGTACAAGTCCCCGGAGGACACGAGTGAGACAGGCTTC
CCTGATGGCTGCCTGGGCCGGCTGTGGTTCTCGGTGGAGTACCAACAGGAGTCTGAGAGGCTGCTGGTGG
GCCTGATCAAGGCCCAGCAACTGCAAGTCCCCTCTGAGACCTGCAGCACCTTGGTGAAGCTCCACCTGCT
GCCTGATGAACGGCGCTTCCTCCAGTCCAAGACCAAACACAAAATTTGTAACCCACAGTTTGATGAGAAC
TTCATCTTCCAGGTATCCAGCAAAAGTGTCACCCAGAGAGTGCTGAAGTTCTCCGTGTACCATGTAAACA
AGAAGAGGAAGCACCAGCTCCTGGGACAGGTGCTATTCCCATTGAAGAATGAGACTCTGGCAGGGGACCA
CCACCGTATCATCTGGAGAGACCTGGAAGCTAAGAACCTGGAGCCCCCCTCGGAGTTTGGTGATATCCAG
TTCTGCCTCAGCTACAACGACTACCTGAGCCGCCTGACTGTGGTCGTGCTCCGAGCCAAGGGTCTCCAGC
TCCAGGAGGACAGAAGTGTTGTCAGTGTGTTTGTCAAGGTGTCTCTGATGAATCACAACAAATTCGTCAA
GTGCAAGAGGACTTCGGCTGTACTGGGCTCCGTCAACCCTGTGTACAATGAAACCTTCAGCTTCAAGGTT
GACACCAATGAGCTGGACACCGCCAGCCTTAGCCTGGTGGTGCTGCAGACCACAGAAGGGAACAAGAGCT
CCCCCCTGGGCCGCGTGGTAGTGGGCCCCTACATGTACACCCGTGGCAAAGAGCTGGAACACTGGGGGGA
GATGCTTCGCAAGCCGAAGGAGCTGGTGAAGCGCTGGCACGCACTCTGCCGCCCCACGGAGCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216214 representing NM_181529
Red=Cloning site Green=Tags(s)

MAEQLAFLIGGIIGGLLLLIGVSCCLWRRFCATFTYEELPETSDPATISYFSRKEDRLYQYSGTPPGRLP
SVPFVVPPSHQGRDWVPLHGGDWAVAPQDPCPVPEHMACTSSAKPGDACEMGSINPELYKSPEDTSETGF
PDGCLGRLWFSVEYQQESERLLVGLIKAQQLQVPSETCSTLVKLHLLPDERRFLQSKTKHKICNPQFDEN
FIFQVSSKSVTQRVLKFSVYHVNKKRKHQLLGQVLFPLKNETLAGDHHRIIWRDLEAKNLEPPSEFGDIQ
FCLSYNDYLSRLTVVVLRAKGLQLQEDRSVVSVFVKVSLMNHNKFVKCKRTSAVLGSVNPVYNETFSFKV
DTNELDTASLSLVVLQTTEGNKSSPLGRVVVGPYMYTRGKELEHWGEMLRKPKELVKRWHALCRPTEP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181529
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181529.4, NP_852682.1
RefSeq Size 3625 bp
RefSeq ORF 1257 bp
Locus ID 319508
UniProt ID Q8C6N3
Cytogenetics 14 B
Summary May be involved in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181529" in other vectors (4)
SKU Description Size Price
MC214412 Syt15 (untagged) - Mouse synaptotagmin XV (Syt15), transcript variant a, (10ug) 10 ug
$503.00
MR216214 Syt15 (Myc-DDK-tagged) - Mouse synaptotagmin XV (Syt15), transcript variant a 10 ug
$289.00 $457.00
MR216214L3 Lenti ORF clone of Syt15 (Myc-DDK-tagged) - Mouse synaptotagmin XV (Syt15), transcript variant a 10 ug
$757.00
MR216214L4 Lenti ORF clone of Syt15 (mGFP-tagged) - Mouse synaptotagmin XV (Syt15), transcript variant a 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.