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Phc3 (NM_001165956) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216212
Phc3 (tGFP-tagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3) transcript variant 4, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,072.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phc3
Synonyms E030046K01Rik; Edr3; Hph3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216212 representing NM_001165956
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGCGGAGTTTAAGGACCACAGCACAGCTATGGATAGTGAGCCGAGCTCTGGAACATCCGTGT
CAACAACAGCCAGCAGTACCACCACCACCACCATCACCACATCCTCCTCCCGAATGCAGCAGCCACAGAT
CTCTGTCTACAGCGGGTCAGACCGACATGCTGTTCAGGTAATTCAGCAGGCCTTGCATCGGCCCCCCAGC
TCGGCAGCTCAGTACCTGCAGCAGATGTACGCGGCTCAGCAGCAGCACCTGATGCTGCACACGGCTGCTC
TGCAGCAGCAGCACCTAAGCAGCTCGCAGCTCCAGAGCCTTGCGGCTGTCCAGGCAAGTTTGTCCAGTGG
AAGACCATCTACATCCCCCACAGGCAGTGTTACACAACAGTCAAGTATGTCCCAGACATCTATCCTGTCC
GCTTCTCCTGCACCTGCTCAGCTGATGAACCGCTCCCAGACGTCCAGTTCTACCAGCGGCAGTATTACCC
AGCAGACTATGCTACTCGGGAGTACCTCTCCCACCCTAACTGCCAGCCAAGCCCAGATGTATCTCCGAGC
TCAGATGCTGATTTTCACACCTGCTACCACTGTGGCTGCTGTACAGTCTGACATCCCTGTTGTCTCGTCG
TCACCGTCACCTTCCTGTCAGTCTGCAGCTGCTCAGGTTCAGAATTTAACCTTACGCAGCCAGAAGCTGG
GTGTCTTATCTAGCTCACAGAATGGCTCACCAAAAAGCGCTGGTCAAACCCAGTCCTTGACAATTTGCCA
TAATAAAACAACAGTGACCAGTTCTAAAATCAGCCAACGAGACCCTTCTCCAGAGAGCAAGAAGGGAGGA
AGCCCAGGTCTGGAATCTCGGAGCACGGCCGTCACTCGGACATCAAGCATCCACCAGTTAATAGCACCAG
CTTCATATTCTCCAATTCAGCCTCATTCTCTAATCAAACATCAGCAGATTCCTCTTCATTCACCACCTCC
TAAAGTTTCCCATCATCAGCTGCTATTACAACAGCAACAACAGCAAATTCAGCCAATCACCCTTCAGAGT
CCAAGTCAAGATCCACCCCCATCCCAGCACTGTATCCCACTCCCAAACCATGGCCTTTCTCCGGCTCCCA
GTAATGCCCAGCCGCAGCATTGCTCCCCAGTTCAGAGCCATCCTCCGCCTTTAACTGTGTCTCCCAACCA
GGCACAGTCCGCACAGCAGTCTGTAGTGGTGTCTCCTCCCCCCCCTCATTCCCCAAGTCAGTCTCCTACT
ATAATTATCCATCCACAAGCACTTATCCAGCCACACCCTCTTGTGTCTTCAGCTCTTCAAACAGGGCCAA
ATTTGCAGCAGATGGTGTCCCCAACATCACACCAGCAGTATTCAGCCCTACAGTCCTCTCCAATTCCCAT
CGCGACCCCTCCACAGATGTCGGCATCTCCTCCAGCTCAGCTCCCACCACTGCCCTTGCAGTCTATGCAG
TCTTTACAAGTGCAGCCTGAAATTCTATCCCAGGGCCAGGTTTTGGTGCAGAATGCTTTGGTGTCAGAAG
AGGAGCTACCTGCTGCAGAAGCTTTGGTCCAGTTGCCATTTCAGACTCTTCCTCCTCCACAGACTGTTGC
GGTAAATCTACAAGTACAGCCGCCAGCACCTGTTGATCCACCAGTGGTTTATCAAGTAGAAGATGTGTGT
GAGGAAGAAATGCCAGAGGAGTCCGATGAGTGTGCAAGGATGGACAGAACCCCCCCACCCCCCACCTTGT
CTCCAGCAGCTGTCACTGTGGGGAGGGGAGAGGACTTGACATCCGAACATCCTTTGTTAGAGCAAGTGGA
GTTGCCTGCTGTGGCCTCAGTCAGTGCTTCCGTGATCAAGTCTCCATCAGACCCCACCCATGCCTCTGCT
CCTGCACCTCCCCTCTTAATCCCTGCTGCCTCCACCAGGAGCAGCAGCACTTCTCTGGCCAGCAGCACTC
CCAGCCTGGAAAACAAGCCTCCACAGGCTATTGTGAAACCACAGATTTTGACCCACGTTATAGAAGGCTT
TGTGATTCAGGAGGGGCTGGAGCCGTTTCCTGTGAGTCGATCATCACTGCTGATAGAACAACCTGTGAAG
AAGAGGCCTCTCTTGGATAATCAGGTGGTAAACTCTGTGTGTGTCCAGCCAGAGTTACAGAATAACACAA
AGCATGCAGATAACTCATCCGACACAGAGATAGAAGACATGATGGCTGAAGAGACATTGGAAGAAATGGA
TAGCGAGTTACTCAAGTGTGAATTCTGTGGAAAAATGGGATATCCTAATGAATTTTTACGGTCCAAACGA
TTCTGTACTATGTCATGTGCCAAAAGGTACAACGTTAGCTGTTCTAAAAAATTTGCACTTAGTCGTTGGA
ATCGTAAGCCTGATAATCAGAGTCTTGGGCATCGGGGCCGTCGTCCAAGTGGCCCTGAAGGGGCAGCAAG
AGAACATATCCTTAGGCAGCTTCCAATTACTTATCCATCTGCAGAAGAAGACGTGGCCTCTCATGAAGAT
CCTGTGCCTTCTGCTATGACGACCCGTCTGCGAAGGCAGAGCGAGCGGGAAAGAGAGCGAGAGCTTCGGG
ATGTGAGGATTAGGAAGATGCCTGAGAATAGTGACTTGCTCCCAGTCGCACAGACTGAGCCCTCCATATG
GACAGTCGACGACGTCTGGGCCTTCATCCATTCTTTGCCCGGCTGCCAGGACGTTGCAGATGAGTTCAGG
GCACAAGAGATTGATGGGCAGGCCCTGCTCTTGCTAAAAGAAGACCATCTCATGAGTGCAATGAACATGA
AGCTGGGCCCAGCCCTGAAGATTTGTGCACGCATCAACTCTCTGAAGGACTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216212 representing NM_001165956
Red=Cloning site Green=Tags(s)

MAEAEFKDHSTAMDSEPSSGTSVSTTASSTTTTTITTSSSRMQQPQISVYSGSDRHAVQVIQQALHRPPS
SAAQYLQQMYAAQQQHLMLHTAALQQQHLSSSQLQSLAAVQASLSSGRPSTSPTGSVTQQSSMSQTSILS
ASPAPAQLMNRSQTSSSTSGSITQQTMLLGSTSPTLTASQAQMYLRAQMLIFTPATTVAAVQSDIPVVSS
SPSPSCQSAAAQVQNLTLRSQKLGVLSSSQNGSPKSAGQTQSLTICHNKTTVTSSKISQRDPSPESKKGG
SPGLESRSTAVTRTSSIHQLIAPASYSPIQPHSLIKHQQIPLHSPPPKVSHHQLLLQQQQQQIQPITLQS
PSQDPPPSQHCIPLPNHGLSPAPSNAQPQHCSPVQSHPPPLTVSPNQAQSAQQSVVVSPPPPHSPSQSPT
IIIHPQALIQPHPLVSSALQTGPNLQQMVSPTSHQQYSALQSSPIPIATPPQMSASPPAQLPPLPLQSMQ
SLQVQPEILSQGQVLVQNALVSEEELPAAEALVQLPFQTLPPPQTVAVNLQVQPPAPVDPPVVYQVEDVC
EEEMPEESDECARMDRTPPPPTLSPAAVTVGRGEDLTSEHPLLEQVELPAVASVSASVIKSPSDPTHASA
PAPPLLIPAASTRSSSTSLASSTPSLENKPPQAIVKPQILTHVIEGFVIQEGLEPFPVSRSSLLIEQPVK
KRPLLDNQVVNSVCVQPELQNNTKHADNSSDTEIEDMMAEETLEEMDSELLKCEFCGKMGYPNEFLRSKR
FCTMSCAKRYNVSCSKKFALSRWNRKPDNQSLGHRGRRPSGPEGAAREHILRQLPITYPSAEEDVASHED
PVPSAMTTRLRRQSERERERELRDVRIRKMPENSDLLPVAQTEPSIWTVDDVWAFIHSLPGCQDVADEFR
AQEIDGQALLLLKEDHLMSAMNMKLGPALKICARINSLKDS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001165956
ORF Size 2853 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001165956.1, NP_001159428.1
RefSeq Size 10925 bp
RefSeq ORF 2856 bp
Locus ID 241915
Cytogenetics 3 A3
Summary Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001165956" in other vectors (4)
SKU Description Size Price
MC222815 Phc3 (untagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 4, (10ug) 10 ug
$959.00
MR216212 Phc3 (Myc-DDK-tagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 4 10 ug
$589.00 $872.00
MR216212L3 Lenti ORF clone of Phc3 (Myc-DDK-tagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 4 10 ug
$1,172.00
MR216212L4 Lenti ORF clone of Phc3 (mGFP-tagged) - Mouse polyhomeotic-like 3 (Drosophila) (Phc3), transcript variant 4 10 ug
$1,172.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.