Arhgap44 (NM_001099288) Mouse Tagged ORF Clone

CAT#: MG216207

Arhgap44 (tGFP-tagged) - Mouse expressed sequence AU040829 (AU040829) transcript variant 1, (10ug)

Product Images

Circular map for MG216207

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgap44
• Synonyms: 6330543G20; AI840762; AU040829; AW493732; Rich2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216207 representing NM_001099288
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGCAGTTTAACCGCATGCGCCAGTTGGCCAACCAGACGGTGGGCAGGGCAGAAAAGACTGAAG
  TTCTGAGCGAAGACCTTCTCCAGGTGGAGAAGCGCCTAGAGCTGGTGAAGCAGGTGTCCCACAGCACACA
  CAAGAAGCTCACCGCATGTCTGCAGGGTCAGCAGGGAGCAGAGGCCGACAAGCGATCAAAAAAGTTGCCT
  CTGACGACTCTGGCGCAGTGCTTGGTGGAGGGGTCCGCGATCCTGGGCGATGACACGCTTCTTGGGAAGA
  TGCTGAAGCTCTGCGGAGAGACGGAGGACAAGCTAGCTCAGGAGCTGATCCACTTTGAGCTGCAAGTGGA
  GAGGGATGTGATCGAGCCCCTGTTCCTGCTCGCTGAGGTGGAAATTCCAAATATTCAAAAGCAGAGGAAA
  CATTTAGCCAAGCTGGTGCTTGACATGGACTCTTCCCGGACCAGGTGGCAGCAGACTTCCAAGTCTTCAG
  GGCTTTCCAGCAGCCTTCAGCCTGCGGGTGCCAAAGCCGATGCCCTCAGGGAGGAGATGGAGGAGGCTGC
  CAACAGAGTGGAGATTTGCCGGGACCAGCTTTCTGCTGACATGTACAGTTTTGTGGCCAAAGAAATTGAC
  TATGCAAACTACTTTCAAACGCTAATAGAAGTGCAAGCTGAGTACCACAGGAAGTCGCTGACACTGCTGC
  AGGCTGTGTTGCCTCAGATCAAAGCCCAGCAGGAGGCCTGGGTGGAGAAGCCTTCCTTTGGGAAGCCCCT
  GGAGGAGCACCTGATGATCAGTGGCCGAGAGATCGCCTTCCCTATCGAGGCGTGTGTGACCATGCTGCTG
  GAGTGTGGGATGCAGGAGGAGGGCCTCTTCCGGGTAGCCCCCTCAGCCTCCAAGCTGAAGAAGCTGAAAG
  CTGCTCTGGACTGCTGTGTGGTGGATGTCCAGGAGTACTCGGCTGACCCCCACGCCATAGCAGGGGCTTT
  GAAGTCTTATCTCCGAGAGTTGCCAGAGCCTCTTATGACCTTTGAACTCTATGACGAATGGATCCAGGCT
  TCTAATATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCA
  ACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGAT
  GACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACC
  GAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGT
  TCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGC
  CAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGG
  CCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGCATGGGTGTGAGGGTCA
  TGGACACATCTTGGGTGGCACGAAGAGGCTCCTCTGCTGGTCGGAAAGCATCCTGCGCCCCGCCTTCCAT
  GCAGCCTCCTGCCCCACCCTCCGAGCTGGCTGCACCCTTGCCTTCACCCCTGCCGGAGCAGGTCCCAGAC
  AGCCCTGCCACTCCAGCTCCTGCGCTCTCTCCATCAGGTGCCAGCCTGCAGCCCACCCCCGAGCGCCCCA
  GTGTGTCTAAAAGCAAGGAACTGTCGCCAGGGTCTGGGCAGAAAGGAAGTCCAGGTTCCATCCAGGGGAC
  ACCCTGCCCAGGGACGCAACTGGGGCCACAGCCGGCTGCCAGCCCCAGCCAGCTTCCTGCAGACCAGAGT
  CCTCACACCCTCCGGAAAGTTTCAAAGAAGGTGGCACCAATTCCTCCCAAGGTCCCCTTCGTGCAGCCAG
  GGACTGTTTCAGATCAGCCCGTTGGCCAGCCATCCCCAGTCAGCCTGTCTCCTACTCCACCAAGCACCCC
  ATCCCCCTATGGACTGAGCTACCCTCCAGGCTACTCCATGGCCTCTGGCCAGCTTTCTCCTGCCTCAGCT
  CCTCCCCTGGCCTCTCCCTCTGTCTTTACAAGCACTTTAGCCAAATCTCGGCCCACCCCTAAGCCTCGAC
  AGAGGCCCACCCTGCCGCCTCCTCAGCCTCCCAGTGTCAGCCTGTCGGCTTCAAGTCCACAGTCCACAGA
  GCACCCCATGTTAGACGGCATGTCGCCGGGGGAAAGCATGTCTACAGATCTCGTCCACTTTGATGTCCCC
  TCCATCCACATAGAGCTGGGGTCAACGCTCCGCCTGAGCCCCCTGGAACATGCACGGCGGCACTCGGCAA
  CAGACAAGAGGGACTCGGAAGAGGAGTCTGAGAGTACAGCCCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216207 representing NM_001099288
  Red=Cloning site Green=Tags(s)
  
  MKKQFNRMRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSHSTHKKLTACLQGQQGAEADKRSKKLP
  LTTLAQCLVEGSAILGDDTLLGKMLKLCGETEDKLAQELIHFELQVERDVIEPLFLLAEVEIPNIQKQRK
  HLAKLVLDMDSSRTRWQQTSKSSGLSSSLQPAGAKADALREEMEEAANRVEICRDQLSADMYSFVAKEID
  YANYFQTLIEVQAEYHRKSLTLLQAVLPQIKAQQEAWVEKPSFGKPLEEHLMISGREIAFPIEACVTMLL
  ECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQA
  SNIQEQDKRLQALWNACEKLPKANHNNIKYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQSEGNIT
  EMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNLTGSYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARR
  PLSVATDNMMLEFYKKDGMGVRVMDTSWVARRGSSAGRKASCAPPSMQPPAPPSELAAPLPSPLPEQVPD
  SPATPAPALSPSGASLQPTPERPSVSKSKELSPGSGQKGSPGSIQGTPCPGTQLGPQPAASPSQLPADQS
  PHTLRKVSKKVAPIPPKVPFVQPGTVSDQPVGQPSPVSLSPTPPSTPSPYGLSYPPGYSMASGQLSPASA
  PPLASPSVFTSTLAKSRPTPKPRQRPTLPPPQPPSVSLSASSPQSTEHPMLDGMSPGESMSTDLVHFDVP
  SIHIELGSTLRLSPLEHARRHSATDKRDSEEESESTAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099288
• ORF Size: 2424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099288.1, NP_001092758.1
• RefSeq Size: 4047 bp
• RefSeq ORF: 2427 bp
• Locus ID: 216831
• UniProt ID: Q5SSM3
• Cytogenetics: 11 B3
• Gene Summary: GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:24352656, PubMed:26969129). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (PubMed:23739967, PubMed:24352656, PubMed:26969129). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (PubMed:23739967).[UniProtKB/Swiss-Prot Function]