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Gramd4 (NM_172611) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216194
Gramd4 (tGFP-tagged) - Mouse GRAM domain containing 4 (Gramd4), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $789.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gramd4
Synonyms 9930016O13
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216194 representing NM_172611
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCATTGCTTCTCACTCTGCATTCAGAGAGAGAGAGAGCTCACCTACAGGAGCCTCTCTGGATGCCA
GCCCCAGACCCTGGGACAAAGGCCTGTCTGGAAGGGAGCCTCCCCGTCATGTCCAGGTGAGGCCGAGGAG
TGCCGTGCTGAACATGCTCAGGAGGCTGGATAGGATCAGGTTCAGAGGACACAAGAGAGAGGACCTCCTT
GATCTAGCCGAGTCCCCAAATGCCTCGGACACCGAGTGCGGAGACGAGATCCCCCTGAAGACGCCACGGC
CCTCGCCCCGGGACAGTGAGGAGCTCAGGGACCCTGCTGGTCCAGGGACCCTCATCATGGCGGCAGGCGT
GCAGGACTTCAACAGGACAGAGTTCGATCGACTGAACGAGATCAAGGGCCATCTGGAAATCGCCTTACTG
GAAAAGCACTTTCTTCAGGAGGAGCTCCGGAAGCTTCGAGAAGAGACCAACTCAGAGATGCTGCGGCAGG
AGCTGGATCGGGAGCGGCAGCGCCGGATCGAACTGGAACAGAAAATGCAGGAGGTGCTAAAGGCCAGGTC
CGAGGAGCAGCCAGCACAGCCTCAGCAGCCACCAAAGGGACAGAGCCAGGCCAGCAATGGCACAGGCACA
GAGCGCCGCAGCCAGGGCTTGGCCTCCCGTGTACAGAAGTGGTTCTATGAGAGGTTTGGGGAATACATCG
AGGACTTCCGCTTCCAACCAGAGGAGAACACAGTAGAGACGGAGGAGCCCCTCAGCGCCCGCAGGTTAAC
TGAAAACATGAGAAGACTGAAGCGCGGTGCCAAGCCTGTCACTAACTTCGTGAAGAACCTGTCTGCCTTA
TCTGACTGGTACTCCATCTACACCTCCGCCATCGCCTTCACGGTATACATGAATGCTGTGTGGCACGGCT
GGGCCATCCCCATGTTCCTGTTCCTAGCAATTCTGAGGTTGTCCCTCAATTACCTCATCGCCAGGGGCTG
GCGGATACAATGGAGCATTGTGCCGGAAGTGTCTGAAGCTGTGGAACCCGCAAAGGAAGATCTGACCGTG
TCTGAGAAGTTCCAGCTGGTGCTGGATGTGGCCCAGAAAGCACAGAATCTCTTCGGCAAGATGGCCGACA
TCCTGGAAAAGATCAAGAACTTGTTCATGTGGGTGCAACCTGAGACCACGCAGAAGCTCTATGTTGCCCT
GTGGGCCGCCTTTCTGGCTTCTTGCTTCTTCCCCTACCGCCTGGTGGGGCTTGCTGTTGGCCTCTATGCC
GGCATCAAGTTTTTCTTAATTGACTTCATCTTCAAACGCTGCCCAAGACTTCGAGCCAAGTATGACACCC
CCTATATCATCTGGAGGAGCCTGCCCACTGACCCTCAGCTCAAAGAGCGTGCTGGTGCCACCGTGTCACG
CAGGCTGCAGACAGCCTCATCACGGAGCTACGTCTCCAGTGCTCCAGCTGGTCTGAGTAAGGACGAAGAT
GCTGGACGCTTTCACAGCACCAAGAAGGGGAACTTCCACGAAATCTTCAACTTGACTGAAAACGAGCGCC
CGCTAGCAGTGTGCGAGAATGGCTGGCGCTGCTGCCTCATTAACCGAGACCGGAAGATGCCCACGGACTA
CATCAGGAACGGGGTTCTGTATGTGACAGAGAATTACCTGTGCTTCGAGAGCTCCAAGTCTGGGTCATCA
AAGAGGAACAAGGTGATCAAACTGATGGACATCACAGACATCCAGAAGTATAAAGTCTTGTCTGTCCTCC
CTGGCTCAGGCATGGGAATTGCTGTTTCTACACCATCAACCCAGAAACCCCTGGTGTTCGGTGCCATGGT
GCACAGAGATGAAGCCTTTGAGACCATTTTCAGCCAGTATGTGAAAATCACGTCTGCAGCGGCCTCTGGC
GGTGACAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216194 representing NM_172611
Red=Cloning site Green=Tags(s)

MGIASHSAFRERESSPTGASLDASPRPWDKGLSGREPPRHVQVRPRSAVLNMLRRLDRIRFRGHKREDLL
DLAESPNASDTECGDEIPLKTPRPSPRDSEELRDPAGPGTLIMAAGVQDFNRTEFDRLNEIKGHLEIALL
EKHFLQEELRKLREETNSEMLRQELDRERQRRIELEQKMQEVLKARSEEQPAQPQQPPKGQSQASNGTGT
ERRSQGLASRVQKWFYERFGEYIEDFRFQPEENTVETEEPLSARRLTENMRRLKRGAKPVTNFVKNLSAL
SDWYSIYTSAIAFTVYMNAVWHGWAIPMFLFLAILRLSLNYLIARGWRIQWSIVPEVSEAVEPAKEDLTV
SEKFQLVLDVAQKAQNLFGKMADILEKIKNLFMWVQPETTQKLYVALWAAFLASCFFPYRLVGLAVGLYA
GIKFFLIDFIFKRCPRLRAKYDTPYIIWRSLPTDPQLKERAGATVSRRLQTASSRSYVSSAPAGLSKDED
AGRFHSTKKGNFHEIFNLTENERPLAVCENGWRCCLINRDRKMPTDYIRNGVLYVTENYLCFESSKSGSS
KRNKVIKLMDITDIQKYKVLSVLPGSGMGIAVSTPSTQKPLVFGAMVHRDEAFETIFSQYVKITSAAASG
GDS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172611
ORF Size 1899 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172611.4, NP_766199.1
RefSeq Size 4117 bp
RefSeq ORF 1902 bp
Locus ID 223752
UniProt ID Q8CB44
Cytogenetics 15 E2
Summary Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53 (By similarity). Inhibits TLR9 response to nucelic acids and regulates TLR9-mediated innate immune response (PubMed:25917084).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172611" in other vectors (4)
SKU Description Size Price
MC219942 Gramd4 (untagged) - Mouse GRAM domain containing 4 (Gramd4), transcript variant 1, (10ug) 10 ug
$649.00
MR216194 Gramd4 (Myc-DDK-tagged) - Mouse GRAM domain containing 4 (Gramd4), transcript variant 1 10 ug
$289.00 $589.00
MR216194L3 Lenti ORF clone of Gramd4 (Myc-DDK-tagged) - Mouse GRAM domain containing 4 (Gramd4), transcript variant 1 10 ug
$889.00
MR216194L4 Lenti ORF clone of Gramd4 (mGFP-tagged) - Mouse GRAM domain containing 4 (Gramd4), transcript variant 1 10 ug
$889.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.