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Ap4b1 (NM_026193) Mouse Tagged ORF Clone

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SKU
MG216173
Ap4b1 (tGFP-tagged) - Mouse adaptor-related protein complex AP-4 beta 1 (Ap4b1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $877.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ap4b1
Synonyms 1810038H16Rik; Ap4b4; AV004952
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216173 representing NM_026193
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATACCTTGGCTCTGAGGACGTGGTGAAGGAACTGAAGAAGGCTCTGTGTAACCCTCATATTCAGG
CTGATAGGCTGCGCTACCGGAATGTCATCCAGCGAGTTATTAGGCACATGACTCAGGGCTTGGACATGTC
TGATGTTTTCATGGAAATGGTAAAGGCCAGTGCCACTGTAGATATTGTTCAGAAAAAGTTGGTTTATCTG
TATATGGGCACATACGCACCTTTAAAACCAGATCTAGCACTCTTGGCCATCAATACGCTCTGCAAAGACT
GCTCAGACCCCAACCCAATGGTGCGAGGACTGGCACTACGGAGTATGTGTAGCCTCAGGATGCCTGGTGT
GCAGGAATATATCCAGCAGCCTGTTGTTAACGGTCTGCGTGATAAAGCTTCATATGTCAGAAGAGTAGCA
GTCCTCGGCTGTGCCAAAATGCATAATCTTCACGGAGACTCTGAAGTGGATGGTGCTCTCGTCAATGAGT
TGTACAGTCTTCTGCGTGACCAGGATCCAATTGTGGTTGTGAACTGTCTGAGGTCTCTAGAGGAAATTTT
AAAGCAGGAAGGAGGTGTTGTCATTAATAAGCCCATTGCCCACCATCTCTTAAATAGAATGTCAAAACTG
GACCAGTGGGGCCAGGCTGAAGTACTGAACTTTCTGCTCCGCTACCAACCCCGCAGTGAGGAGGAACTGT
TTGATATTCTCAATTTGTTGGACAGCTATCTCAAGAGCAGCAGTACTGGCGTGGTGATGGGAGCCACCAA
ATTCTTTCTGATTTTGGCAAAAAAGTTCCCCCACGTGCAGACTGATGTGCTTGTGCGAGTCAAGGGGCCC
TTGCTGGCCGCCTGCTCCTCAGAGAGCCGTGAGCTCTGTTTTGCTGCTCTCTGCCATGTCCGCCAGGTCT
TGCACAGTTTGCCAGGTCACTTTAGCAGTCACTACAAAAAGTTTTTTTGCTCTTACTCGGAACCCCACTA
CATCAAGCTCCAGAAGGTGGAGGTGCTGTGCGAGCTGGTGAATGACGAGAACGTGCAGCAGGTGCTGGAA
GAGCTGCGTGGCTACTGCACTGACGTGGCTGCTGACTTTGCACAGGCTGCCATCTTCGCCATAGGTAGCA
TTGCCAAGACTTACACAGATCAATGTGTGCAGATTCTAACAGAGTTGCTGGGACTTCGACAAGAACATAT
CACTACTGTGGTCGTGCAGACTTTCCGAGACCTGGTCTGGCTATGCCCTCAGTGTACTGAAGCTGTGTGT
CAGGCTCTGCCCGGCTGTGAGGAGAGCATTCAAGACAGTGAGGGGAAACAGGCACTTATTTGGTTACTTG
GGGTCCACGGGGAAAAAATCCCCAATGCTCCTTATGTGTTGGAGGACTTTGTAGACAACGTGAAGTCAGA
GACATTTCCCGCTGTTAAGATGGAGCTACTGACCGCACTGATGCGCCTTGTCCTCTCGCGCCCGGCTGAG
TGCCAGGACATGCTGGGACGGCTGCTGCATTACTGCATAGAGGAAGAAAAGGATATGGCTGTACGGGACC
GGGGTCTCTTCTATTACCGCCTCCTGTTAGTTGGCATTGATAAAGTTAAGCAGATCCTGTGTAGTCCTAA
GTCTGACCCTTCTCTTGGACTTTTGGAGGATCAGCCGGAACGACCAGTAAATAGCTGGGCTTCAGACTTC
AACACACTGGCGCCAGTGTATGGCAGAGCCCACTGGGCAACTATCTCTAAATGCCAGCAGGTAGAGCGGC
ATCGCCTTGAGCTTCCCCACAATGCATCCTTTGCCACATCAGGGCACTTGATTTCCGAGGAGAACAAAAA
AGGTGCACAAGAACCTGATTCTGACACTCTCATGCTGGTCCCTAATCTCCAGCTTACTGCTGAGTATTTT
GAGAAGACGTGGCTTAGCCTCAGAGTTAGTTACCAGCAGGTGTTTCCTTGGCAGGGAGAAGTCCAGCCTG
ACACTCTACAAATGGCTCTAAAAGTAGTGAACATTCAGACCATCGCAATGAGCAGAGCTGGGGCACAGCC
CTGGAAAGCCTACCTCAGTGCTCAGGATGATACTGGCGGCCTTTTCTTAGCTGAATTGCTACTGAAGCCT
GAGAACTCAGAGATGCAAATCTCTGTGAAGCAAAGTAAGGCAAGGACTGAATCCCTGCATGGTTTTGTTT
CTGTCCTGGAAACTGTGATTGGAACAGTTGGAGACATAAAATCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216173 representing NM_026193
Red=Cloning site Green=Tags(s)

MPYLGSEDVVKELKKALCNPHIQADRLRYRNVIQRVIRHMTQGLDMSDVFMEMVKASATVDIVQKKLVYL
YMGTYAPLKPDLALLAINTLCKDCSDPNPMVRGLALRSMCSLRMPGVQEYIQQPVVNGLRDKASYVRRVA
VLGCAKMHNLHGDSEVDGALVNELYSLLRDQDPIVVVNCLRSLEEILKQEGGVVINKPIAHHLLNRMSKL
DQWGQAEVLNFLLRYQPRSEEELFDILNLLDSYLKSSSTGVVMGATKFFLILAKKFPHVQTDVLVRVKGP
LLAACSSESRELCFAALCHVRQVLHSLPGHFSSHYKKFFCSYSEPHYIKLQKVEVLCELVNDENVQQVLE
ELRGYCTDVAADFAQAAIFAIGSIAKTYTDQCVQILTELLGLRQEHITTVVVQTFRDLVWLCPQCTEAVC
QALPGCEESIQDSEGKQALIWLLGVHGEKIPNAPYVLEDFVDNVKSETFPAVKMELLTALMRLVLSRPAE
CQDMLGRLLHYCIEEEKDMAVRDRGLFYYRLLLVGIDKVKQILCSPKSDPSLGLLEDQPERPVNSWASDF
NTLAPVYGRAHWATISKCQQVERHRLELPHNASFATSGHLISEENKKGAQEPDSDTLMLVPNLQLTAEYF
EKTWLSLRVSYQQVFPWQGEVQPDTLQMALKVVNIQTIAMSRAGAQPWKAYLSAQDDTGGLFLAELLLKP
ENSEMQISVKQSKARTESLHGFVSVLETVIGTVGDIKS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026193
ORF Size 2214 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026193.2, NP_080469.2
RefSeq Size 2914 bp
RefSeq ORF 2217 bp
Locus ID 67489
UniProt ID Q9WV76
Cytogenetics 3 F2.2
Summary Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (By similarity). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (PubMed:18341993). AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026193" in other vectors (5)
SKU Description Size Price
MC202043 Ap4b1 (untagged) - Mouse adaptor-related protein complex AP-4, beta 1 (Ap4b1), transcript variant 1, (10ug) 10 ug
$677.00
MG210377 Ap4b1 (tGFP-tagged) - Mouse adaptor-related protein complex AP-4, beta 1 (Ap4b1) 10 ug
$877.00
MR216173 Ap4b1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, beta 1 (Ap4b1), transcript variant 1 10 ug
$589.00 $677.00
MR216173L3 Lenti ORF clone of Ap4b1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, beta 1 (Ap4b1), transcript variant 1 10 ug
$977.00
MR216173L4 Lenti ORF clone of Ap4b1 (mGFP-tagged) - Mouse adaptor-related protein complex AP-4, beta 1 (Ap4b1), transcript variant 1 10 ug
$977.00

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