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Lin54 (NM_001115010) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216162
Lin54 (tGFP-tagged) - Mouse lin-54 homolog (C. elegans) (Lin54) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $886.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lin54
Synonyms AI461788
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216162 representing NM_001115010
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGTGCCGGCCGAGGTGAATAGTTTGCTTCCAGACGACATAATGGACACTGCTATAACGTTAG
TGGATGAGGATAGTATTGAGGCTGTAATTGTGTCATCCCCAATTCCCATGGAGACAGAGCTGGAAGAAAT
TGTCAACATAAATTCTACCGGTGACTCTACAGCCACGCCCATTTCCACGGAACCAATCACAGTGTACAGT
AACCACACTAACCAAGTTGCAGTGAATACCACAGTTTCTAAAGCAGATTCTAATACCACAGTGAAACCAG
CATTTCCAAGTGGCCTTCAAAAACTTGGTGCTCAGACTCCTGTGACTATATCAGCCAATCAGATTATTTT
AAACAAAGTATCACAGACATCTGATCTTAAACTTGGCAATCAGACCCTTAAACCAGATGGACAGAAGTTA
ATATTAACAACTTTGGGCAAGTCTGGTTCACCAATTGTTTTAGCACTACCCCATAGCCAACTACCCCAGG
CTCAGAAAGTTACAGCTCAGGCCCAGCCAGGAGATGCTAAGTTACCACCGCAGCAAATTAAAGTAGTTAC
CATTGGAGGGAGGCCAGAGGTGAAACCTGTCATTGGTGTCTCAGCACTGACCCCAGGAAGCCAACTGATT
AATACTACAACTCAGCCCTCTGTGTTACAGACCCAACAGTTAAAAACAGTACAGATTGCTAAGAAGCCTC
GGACACCAACCTCGGGTCCAGTGATCACGAAGCTGATTTTTGCAAAACCAATTAATAGCAAAGCAGTTAC
AGGACAGACAACTCAAGCATCTCCACCCGTCGTCACAGGTAGGGTTCTTTCACAGTCTACTCCTGGAACT
CCATCAAAGACTATAACAATATCAGAAAGTGGTGTGATTGGATCAACTTTAAATTCGACAACACAGACAC
CAAATAAGATAGCCATCTCACCTTTGAAGTCGCCAAATAAGACAGTGAAATCGGCTGTGCAGACCATCAC
TGTGGGAGGAATGAGCACCTCACAGTTTAAGACCATCATCCCTCTGGCCACTGCTCCCAATGTCCAGCAG
ATTCAAGTGCCTGGAAGCAAGTTTCATTATGTCCGACTTGTTACTGCCACAACAGCCAGTAGCTCAGCCC
AGCCAGTCAGTCAGAGCCCCAGTGTAAACACACAGCCTCTCCAGCAAGCAAAACCAGTGGTTGTTAATAC
CACCCCTGTACGGATGTCAGTTCCATTTGTCCAAGCTCAGGCCGTTAAGCAAGTTGTTCCAAAACCAATC
AATTCAACTTCACAAATTGTAACTACCAGCCAGCCACAGCAGCGGCTCATCATGCCTGCCACACCACTGC
CTCAGATCCAGCCCAACCTCACTAACCTGCCACCAGGTACTGTGCTGGCACCAGCTCCAGGAACAGGGAA
TGTGGGCTATGCAGTGCTTCCAGCTCAGTATGTTACTCAGTTACAGCAGTCTTCATATGTGTCTATAGCA
AGCAACTCTAATTTTACTGGAACATCTGGCATCCAGACACAGGCAAGGCTTCCATTCAATGGCATAATTC
CATCAGAGTCTACCAGTCGGCCCAGAAAACCCTGTAACTGTACAAAATCACTGTGTTTGAAATTATACTG
TGATTGCTTTGCAAATGGTGAATTCTGCAACAACTGCAACTGTACCAATTGTTACAACAATTTAGAGCAC
GAGAATGAGAGACAAAAGGCAATCAAGGCATGCCTTGATAGAAATCCGGAAGCCTTCAAGCCAAAGATAG
GAAAAGGAAAAGAGGGGGAGTCGGATCGGCGGCACAGCAAAGGCTGCAACTGCAAACGCTCGGGGTGTCT
TAAAAACTACTGCGAGTGCTATGAGGCAAAAATAATGTGTTCTTCTATATGCAAATGCATCGGCTGTAAG
AATTTTGAAGAAAGCCCAGAACGGAAGACATTGATGCACTTAGCAGATGCGGCTGAAGTAAGGGTCCAGC
AGCAGACAGCAGCGAAGACCAAGCTGTCCTCTCAGATCTCAGACCTCCTTACAAGGCCAACCCCAGCTCT
CAACAGTGCAGGAGGGAAATTGCCGTTTACATTTGTAACTAAAGAAGTAGCTGAAGCCACGTGTAATTGC
CTTCTTGCCCAGGCAGAGCAGGCAGACAAGAAGGGGAAATCAAAGGCGGCAGCAGAGCGGATGATACTTG
AGGAATTTGGACGATGTTTGATGAGCGTCATCAACTCTGCAGGAAAAGCCAAAAGTGACCCCTGTGCCAT
GCACTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216162 representing NM_001115010
Red=Cloning site Green=Tags(s)

MEVVPAEVNSLLPDDIMDTAITLVDEDSIEAVIVSSPIPMETELEEIVNINSTGDSTATPISTEPITVYS
NHTNQVAVNTTVSKADSNTTVKPAFPSGLQKLGAQTPVTISANQIILNKVSQTSDLKLGNQTLKPDGQKL
ILTTLGKSGSPIVLALPHSQLPQAQKVTAQAQPGDAKLPPQQIKVVTIGGRPEVKPVIGVSALTPGSQLI
NTTTQPSVLQTQQLKTVQIAKKPRTPTSGPVITKLIFAKPINSKAVTGQTTQASPPVVTGRVLSQSTPGT
PSKTITISESGVIGSTLNSTTQTPNKIAISPLKSPNKTVKSAVQTITVGGMSTSQFKTIIPLATAPNVQQ
IQVPGSKFHYVRLVTATTASSSAQPVSQSPSVNTQPLQQAKPVVVNTTPVRMSVPFVQAQAVKQVVPKPI
NSTSQIVTTSQPQQRLIMPATPLPQIQPNLTNLPPGTVLAPAPGTGNVGYAVLPAQYVTQLQQSSYVSIA
SNSNFTGTSGIQTQARLPFNGIIPSESTSRPRKPCNCTKSLCLKLYCDCFANGEFCNNCNCTNCYNNLEH
ENERQKAIKACLDRNPEAFKPKIGKGKEGESDRRHSKGCNCKRSGCLKNYCECYEAKIMCSSICKCIGCK
NFEESPERKTLMHLADAAEVRVQQQTAAKTKLSSQISDLLTRPTPALNSAGGKLPFTFVTKEVAEATCNC
LLAQAEQADKKGKSKAAAERMILEEFGRCLMSVINSAGKAKSDPCAMHC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001115010
ORF Size 2247 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001115010.1, NP_001108482.1
RefSeq Size 4596 bp
RefSeq ORF 2250 bp
Locus ID 231506
UniProt ID Q571G4
Cytogenetics 5 E4
Summary Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner. Specifically recognizes the consensus motif 5'-TTYRAA-3' in target DNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001115010" in other vectors (4)
SKU Description Size Price
MC221330 Lin54 (untagged) - Mouse lin-54 homolog (C. elegans) (Lin54), transcript variant 1, (10ug) 10 ug
$755.00
MR216162 Lin54 (Myc-DDK-tagged) - Mouse lin-54 homolog (C. elegans) (Lin54), transcript variant 1 10 ug
$589.00 $686.00
MR216162L3 Lenti ORF clone of Lin54 (Myc-DDK-tagged) - Mouse lin-54 homolog (C. elegans) (Lin54), transcript variant 1 10 ug
$986.00
MR216162L4 Lenti ORF clone of Lin54 (mGFP-tagged) - Mouse lin-54 homolog (C. elegans) (Lin54), transcript variant 1 10 ug
$986.00

file-alt Citations

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