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Asap2 (NM_001004364) Mouse Tagged ORF Clone

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SKU
MG216157
Asap2 (tGFP-tagged) - Mouse ArfGAP with SH3 domain ankyrin repeat and PH domain 2 (Asap2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,511.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Asap2
Synonyms 6530401G17Rik; Ddef2; Gm592; Gm1523; PAG3; PAP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216157 representing NM_001004364
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT
CTAGCTTCACCACGCGCACGGCCCAGTGCCGGAACACCGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA
CCGGATGGTTCTCTACAAAATGAAGAAATCTGTGAAGGCAATCAATATCTCTGGGCTGGCACACGTGGAG
AACGAGGAACAGTACACCCAGGCTCTGGAGAAGTTCGGTGGCAACTGTGTGTGCAGAGATGATCCAGACT
TAGGAAGCGCGTTTCTGAAGTTCTCTGTGTTCACCAAGGAGTTGACGGCACTCTTCAAAAACCTGATTCA
GAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGG
GATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGA
AAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGAT
GGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTG
AAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATG
GATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCATCAAACA
GGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAA
CAGAAAGAGGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGC
ATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAA
GTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAAC
CTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGA
CGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGA
AGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACC
AAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATC
CGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGG
GGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGCTTGCCAAG
AATATCGGGAATGCAGGCTTTAATGAAATTATGGAGTGTTGCCTACCGTCTGAGGACCCAGTCAAGCCCA
ACCCAGGCAGTGACATGATTGCAAGGAAGGACTACATCACAGCCAAGTACATGGAGAGGAGATATGCACG
GAAAAAGCATGCCGACACCGCGGCGAAGCTCCACAGCCTTTGCGAGGCCGTCAAGACCAGAGACATTTTT
GGGTTACTCCAAGCTTATGCTGATGGTGTGGACCTGACAGAGAAAATCCCACTGGCCAATGGGCATGAGC
CAGATGAGACAGCCCTCCATCTTGCAGTCAGATCTGTGGACCGGACTTCCCTTCACATTGTAGACTTCCT
GGTCCAGAACAGTGGGAACCTGGATAAACAGACAGGCAAGGGCAGCACAGCCCTGCACTACTGCTGCCTG
ACAGACAATGCTGAGTGCCTCAAGCTCCTCCTGCGGGGAAAAGCCTCCATTGAGATAGCCAATGAGTCAG
GAGAGACCCCGTTGGACATTGCTAAGCGGCTCAAGCACGAACACTGTGAGGAACTGCTCACTCAGGCCTT
GTCGGGGCGGTTTAACTCCCATGTTCACGTCGAGTATGAATGGCGGCTGCTGCACGAGGACCTGGATGAG
AGCGACGACGATGTGGACGAGAAGCTACAGCCTAGTCCCAACCGACGGGAGGACCGGCCTGTTAGCTTCT
ACCAGCTGGGGTCCAGCCAGTTTCAGTCCAATGCTGTGTCTTTGGCCAGAGATACTGCAAACCTCACCAA
GGACAAGCAGAGGGGCTTTGGGCCCAGCATCTTGCAGAATGAGACCTATGGAGCCATCCTGAGTGGCAGC
CCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGAATGTTGGCA
AAGATCCCCTGACCACCACACCACCCCCTCCTGTGGCTAAGACATCAGGAACTCTAGAAGCCATGAACCA
GCCGAGCAAGTCCTCCCAACCTGGGACCTCACAGAGCAAGCCCCCGCCCCTGCCACCTCAGCCACCCAGC
CGCCTGCCTCAGAAGAAACCAGCTTCCGGGACTGACAAGCCAACCCCGCTGACCAACAAAGGACAGCCGA
GAGGACCTGAAGCTTCGGGTCCTCTGTCCAACGCCATGGCCCTGCAGCCCCCAGCACCCATGCCTAGGAA
GTCACAGGCGACCAAGTCAAAGCCCAAGCGAGTGAAAGCACTCTACAACTGCGTGGCTGACAACCCAGAC
GAGCTGACTTTCTCTGAGGGCGACGTGATCATTGTGGATGGGGAAGAGGATCAGGAGTGGTGGATCGGCC
ACATCGATGGAGAGCCCAGCCGCAAAGGAGCGTTTCCCGTATCGTTTGTGCACTTTATTGCTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216157 representing NM_001004364
Red=Cloning site Green=Tags(s)

MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINISGLAHVE
NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG
DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKV
KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE
QKEDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLN
LLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELT
KEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAK
NIGNAGFNEIMECCLPSEDPVKPNPGSDMIARKDYITAKYMERRYARKKHADTAAKLHSLCEAVKTRDIF
GLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCL
TDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDE
SDDDVDEKLQPSPNRREDRPVSFYQLGSSQFQSNAVSLARDTANLTKDKQRGFGPSILQNETYGAILSGS
PPSSQSIPPSTTSAPPLPPRNVGKDPLTTTPPPPVAKTSGTLEAMNQPSKSSQPGTSQSKPPPLPPQPPS
RLPQKKPASGTDKPTPLTNKGQPRGPEASGPLSNAMALQPPAPMPRKSQATKSKPKRVKALYNCVADNPD
ELTFSEGDVIIVDGEEDQEWWIGHIDGEPSRKGAFPVSFVHFIAD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004364
ORF Size 2865 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004364.2, NP_001004364.2
RefSeq Size 5557 bp
RefSeq ORF 2868 bp
Locus ID 211914
UniProt ID Q7SIG6
Cytogenetics 12 A1.2-A1.3
Summary Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001004364" in other vectors (4)
SKU Description Size Price
MC202979 Asap2 (untagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 2, (10ug) 10 ug
$1,112.00
MR216157 Asap2 (Myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 2 10 ug
$1,311.00
MR216157L3 Lenti ORF clone of Asap2 (Myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 2 10 ug
$1,611.00
MR216157L4 Lenti ORF clone of Asap2 (mGFP-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 2 10 ug
$1,611.00

file-alt Citations

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