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Pde1c (NM_001159953) Mouse Tagged ORF Clone

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SKU
MG216137
Pde1c (tGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c) transcript variant 4, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,234.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde1c
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216137 representing NM_001159953
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCTCCAACCAAGGAAATTGAAGAATTCGAGAGCAACTCTCTGAAACACCTGCAACCTGAGCAGA
TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGGTTACGGTCTTT
GGTCAAGCAATTAGAACGAGGAGAGGCCTCTGTGGTAGACCTTAAGAAGAATCTGGAATATGCAGCCACA
GTGCTTGAATCTGTATATATTGATGAAACAAGGCGACTGCTGGATACAGAGGATGAGCTTAGTGACATTC
AATCAGATGCTGTGCCTTCAGAAGTTCGGGATTGGCTGGCCTCCACCTTCACACGGCAGATGGGGATGAT
GCTTAGGAGAAGTGATGAGAAGCCCAGGTTCAAGAGCATCGTCCATGCGGTGCAAGCTGGGATATTTGTG
GAAAGAATGTACAGACGGACATCAAACATGGTTGGGCTGAGCTATCCACCGGCTGTAATCGATGCATTGA
AGGATGTGGATACGTGGTCCTTCGATGTCTTTTCCCTCAATGAGGCCAGTGGAGATCATGCACTGAAGTT
CATTTTCTATGAATTACTCACACGTTATGACCTGATCAGCCGTTTTAAGATACCGATTTCTGCACTTGTC
TCATTTGTGGAGGCCCTCGAAGTGGGTTACAGCAAGCACAAAAATCCTTACCATAACCTGATGCATGCAG
CTGACGTCACACAGACTGTGCATTACCTCCTTTATAAGACAGGAGTAGCAAACTGGCTGACAGAGCTGGA
GATCTTTGCAATAATCTTTTCGGCTGCCATCCATGACTATGAACATACTGGAACTACAAACAATTTCCAC
ATTCAGACTCGGTCAGATCCAGCTATCCTGTACAATGACAGATCTGTCCTGGAGAACCACCACCTGAGTG
CAGCCTACCGCCTTCTGCAGGAAGACGAAGAGATGAATATTCTGGTTAACCTCTCAAAGGATGACTGGAG
GGAGTTTCGAACTTTGGTTATTGAGATGGTAATGGCCACAGATATGTCCTGTCATTTCCAGCAAATCAAA
GCCATGAAGACAGCCCTGCAGCAACCAGAAGCAATTGAGAAGCCGAAAGCCTTATCCCTCATGTTACACA
CAGCAGACATCAGTCATCCTGCGAAAGCATGGGACCTGCACCACCGCTGGACCATGTCTCTCCTGGAGGA
GTTCTTTAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCATTTTCTCCTCTTTGTGACAGAAAGTCA
ACCATGGTTGCTCAGTCACAAGTGGGTTTTATTGACTTCATTGTGGAGCCCACCTTCACTGTGCTCACGG
ACATGACCGAGAAGATTGTGAGTCCATTAATTGACGAAAGCTCCCAGACTGGTGGGACAGGGCAGAGGAG
ATCAAGTTTGAACAGCATCAACTCATCAGATGCAAAGCGATCCGGTGTCAAGAGTTCTGGGTCAGATGGA
AGTGCTCCCATCAACAATTCTGTCATTCCTGTTGACTATAAGAGTTTTAAAGCCACTTGGACTGAGGTGG
TGCAGATCAATCGGGAGCGGTGGCGAGCCAAGGTACCCAAAGAAGAAAAAGCCAAGAAGGAAGCTGAAGA
GAAGGCTCGCCTGGCTGCTGAGGAAAAGCAAAAGGAAATGGAAGCCAAAAGCCAAGCTGAACAAGGCACA
ACCAGCAAAGGCGAGAAAAAGACATCAGGAGAAGCCAAAAGTCAAGTCAATGGAACACGCAAGGGTGATA
ACCCTCGTGGGAAGAACTCCAAAGGAGAAAAGGCAGGCGAAAAGCAGCAGAACGGTGACTTGAAAGACGG
TAAAAATAAGGCAGACAAGAAGGATCACTCCAACACCGGAAATGAGTCAAAGAAAACAGATGGTACAAAG
AAGCGTTCTCATGGCTCACCTGCTCCGAGCACTAGTTCCACAAGTCGCATTACCTTGCCAGTCATCAAGC
CTCCTTTGCGTCACTTTAAGCGTCCTGCTTATGCATCTAGCTCCTATGCACCTTCCGTCCCGAAGAAAAC
TGATGATCATCCTGTAAGATACAAGATGCTGGATCAGCGGATCAAGATGAAAAAGATCCAGAACATCTCT
CATCACTGGAACAAAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216137 representing NM_001159953
Red=Cloning site Green=Tags(s)

MESPTKEIEEFESNSLKHLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT
VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV
ERMYRRTSNMVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV
SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH
IQTRSDPAILYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIK
AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS
TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDESSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSDG
SAPINNSVIPVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGT
TSKGEKKTSGEAKSQVNGTRKGDNPRGKNSKGEKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKTDGTK
KRSHGSPAPSTSSTSRITLPVIKPPLRHFKRPAYASSSYAPSVPKKTDDHPVRYKMLDQRIKMKKIQNIS
HHWNKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001159953
ORF Size 2118 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159953.1, NP_001153425.1
RefSeq Size 3907 bp
RefSeq ORF 2121 bp
Locus ID 18575
UniProt ID Q64338
Cytogenetics 6 27.65 cM
Summary Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual-specificity for cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:8810348). Exhibits high affinity for both cAMP and cGMP (By similarity). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001159953" in other vectors (4)
SKU Description Size Price
MC220741 Pde1c (untagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4, (10ug) 10 ug
$971.00
MR216137 Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4 10 ug
$1,034.00
MR216137L3 Lenti ORF clone of Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4 10 ug
$1,334.00
MR216137L4 Lenti ORF clone of Pde1c (mGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 4 10 ug
$1,334.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.