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Prpf38a (NM_172697) Mouse Tagged ORF Clone

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SKU
MG216132
Prpf38a (tGFP-tagged) - Mouse PRP38 pre-mRNA processing factor 38 (yeast) domain containing A (Prpf38a), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prpf38a
Synonyms 2410002M20Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216132 representing NM_172697
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAACCGTACAGTGAAGGATGCCCACAGCATCCATGGCACCAACCCTCAGTATCTGGTGGAGAAGA
TCATCCGGACGCGAATCTATGAATCAAAGTACTGGAAAGAAGAGTGTTTTGGACTTACAGCTGAACTTGT
AGTGGACAAAGCCATGGAGTTAAAGTTCGTTGGTGGTGTGTATGGTGGAAACATAAAGCCAACTCCTTTT
TTGTGTTTAACCTTGAAAATGCTTCAGATTCAACCTGAGAAGGATATCATTGTTGAGTTCATAAAAAATG
AAGATTTCAAGTATGTCCGGATGCTGGGGGCACTTTACATGCGGCTCACAGGAACTGCAATTGATTGCTA
CAAGTACTTGGAGCCTTTGTACAATGACTATCGAAAAATCAAGAGCCAGAACCGAAATGGAGAGTTTGTG
CTGATGCATGTAGATGAGTTCATTTATGAACTCCTGCACAGTGAGAGAGTCTGTGATATCATTTTGCCCC
GGCTACAGAAACGGTATGTGCTGGAAGAAGCTGAGCAACTGGAGCCTCGAGTTAGTGCCCTAGAAGAGGA
CATGGATGATGTGGAGTCTAGTGAGGAAGAGGAGGAGGAAGATGAGAAGCTTGAAAGAGTGCCATCACCT
GACCATCGAAGAAGAAGCTACAGAGATTTGGACAAGCCAAGGCGTTCACCTGCACTGCGCTACAGGAGGA
GCCGGAGTCGTTCTCCCAGAAGGCGGAGTAGATCCCCTAAAAGAAGAAGTCCTTCTCCCCGAAGAGAAAG
GCATAGGAGCAAGAGTCCACGACGCCATCGGAGCAGGTCTAGAGACAGACGACACAGGTCCCGCTCTAAA
TCCCCAGGTCACCACCGTAGTCACAGACATAGAAGCCACTCAAAGTCTCCTGAAAGGTCTAAGAAAAGCC
ACAAGAAGAGCCGGAGGGGAAATGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216132 representing NM_172697
Red=Cloning site Green=Tags(s)

MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELKFVGGVYGGNIKPTPF
LCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFV
LMHVDEFIYELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSP
DHRRRSYRDLDKPRRSPALRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHRSRSK
SPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172697
ORF Size 936 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172697.3, NP_766285.2
RefSeq Size 1519 bp
RefSeq ORF 939 bp
Locus ID 230596
UniProt ID Q4FK66
Cytogenetics 4 C7
Summary Involved in pre-mRNA splicing as a component of the spliceosome.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172697" in other vectors (4)
SKU Description Size Price
MC212884 Prpf38a (untagged) - Mouse PRP38 pre-mRNA processing factor 38 (yeast) domain containing A (Prpf38a), (10ug) 10 ug
$330.00
MR216132 Prpf38a (Myc-DDK-tagged) - Mouse PRP38 pre-mRNA processing factor 38 (yeast) domain containing A (Prpf38a) 10 ug
$289.00 $300.00
MR216132L3 Lenti ORF clone of Prpf38a (Myc-DDK-tagged) - Mouse PRP38 pre-mRNA processing factor 38 (yeast) domain containing A (Prpf38a) 10 ug
$600.00
MR216132L4 Lenti ORF clone of Prpf38a (mGFP-tagged) - Mouse PRP38 pre-mRNA processing factor 38 (yeast) domain containing A (Prpf38a) 10 ug
$600.00

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