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Jmjd7 (NM_001114637) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216128
Jmjd7 (tGFP-tagged) - Mouse jumonji domain containing 7 (Jmjd7), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jmjd7
Synonyms MGC106779
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216128 representing NM_001114637
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCGGCTCTGGAGGCAGTTCGGAGGGCGTTGCAAGAGTTCCCGGCGGCGGCTCGCGACCTCA
ATGTACCTCGTGTTGTGCCCTACCTGGATGAGCCCCCAAGCCCACTCTGCTTCTACCGGGATTGGGTGTG
CCCCAACAGGCCCTGCATTATCCGAAATGCTCTGCAGCACTGGCCAGCCCTCCAGAAGTGGTCCCTGTCC
TACTTAAGAGCCACGGTGGGCTCCACGGAGGTGAGTGTGGCTGTGACTCCAGATGGTTATGCGGACGCGG
TGCGAGGGGACCGCTTTGTGATGCCTGCTGAACGCCGCCTGCCCATAAGCCATGTACTGGATGTGTTGGA
AGGTCGGGCCCAGCACCCAGGAGTCCTCTATGTGCAGAAACAGTGTTCCAACCTGCCCACTGAGCTGCCC
CAGCTTCTGTCTGACATTGAGTCCCATGTGCCCTGGGCCTCTGAGTCACTGGGGAAGATGCCTGATGCCG
TGAACTTCTGGCTGGGTGATGCATCTGCAGTGACATCCTTGCACAAGGACCACTATGAGAATCTGTACTG
TGTAGTCTCTGGCGAGAAACACTTCTTGTTACATCCACCCAGCGACCGGCCCTTCATCCCTTACAATCTC
TACACACCAGCAACCTACCAGCTGACTGAAGAGGGCACTTTTAGGGTGGTGGACGAGGAAGCCATGGAGA
AGGTACCCTGGATCCCACTGGACCCCTTGGCTCCAGACCTGACCCAGTACCCCAGTTACAGCCAGGCACA
GGCCCTTCACTGCACAGTGCGGGCCGGCGAGATGCTGTACCTGCCAGCTCTGTGGTTCCACCATGTCCAG
CAGTCCCACGGCTGTATTGCTGTGAATTTCTGGTATGACATGGAGTATGACCTCAAGTACAGTTACTTTC
AACTGATGGACACCCTCACCAGGGCCACAGGCCTTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216128 representing NM_001114637
Red=Cloning site Green=Tags(s)

MAEAALEAVRRALQEFPAAARDLNVPRVVPYLDEPPSPLCFYRDWVCPNRPCIIRNALQHWPALQKWSLS
YLRATVGSTEVSVAVTPDGYADAVRGDRFVMPAERRLPISHVLDVLEGRAQHPGVLYVQKQCSNLPTELP
QLLSDIESHVPWASESLGKMPDAVNFWLGDASAVTSLHKDHYENLYCVVSGEKHFLLHPPSDRPFIPYNL
YTPATYQLTEEGTFRVVDEEAMEKVPWIPLDPLAPDLTQYPSYSQAQALHCTVRAGEMLYLPALWFHHVQ
QSHGCIAVNFWYDMEYDLKYSYFQLMDTLTRATGLD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001114637
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001114637.1, NP_001108109.1
RefSeq Size 1383 bp
RefSeq ORF 951 bp
Locus ID 433466
UniProt ID P0C872
Cytogenetics 2
Summary Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase (PubMed:28847961) (By similarity). Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961). Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961). Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001114637" in other vectors (4)
SKU Description Size Price
MC214968 Jmjd7 (untagged) - Mouse jumonji domain containing 7 (Jmjd7), (10ug) 10 ug
$330.00
MR216128 Jmjd7 (Myc-DDK-tagged) - Mouse jumonji domain containing 7 (Jmjd7) 10 ug
$289.00 $300.00
MR216128L3 Lenti ORF clone of Jmjd7 (Myc-DDK-tagged) - Mouse jumonji domain containing 7 (Jmjd7) 10 ug
$600.00
MR216128L4 Lenti ORF clone of Jmjd7 (mGFP-tagged) - Mouse jumonji domain containing 7 (Jmjd7) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.