Rbbp5 (NM_172517) Mouse Tagged ORF Clone
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Rbbp5 |
| Synonyms | 4933411J24Rik; C330016J05 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_172517 |
| ORF Size | 1614 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_172517.2, NP_766105.2 |
| RefSeq Size | 3632 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 213464 |
| UniProt ID | Q8BX09 |
| Cytogenetics | 1 E4 |
| Summary | As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_172517" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC218569 | Rbbp5 (untagged) - Mouse retinoblastoma binding protein 5 (Rbbp5), (10ug) | 10 ug |
$502.00
|
|
| MR216121 | Rbbp5 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 5 (Rbbp5) | 10 ug |
$551.00
|
|
| MR216121L3 | Lenti ORF clone of Rbbp5 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 5 (Rbbp5) | 10 ug |
$851.00
|
|
| MR216121L4 | Lenti ORF clone of Rbbp5 (mGFP-tagged) - Mouse retinoblastoma binding protein 5 (Rbbp5) | 10 ug |
$851.00
|
|
Plasmid Map for MG216121
Download sequence for
Cloning scheme for MG216121
Circular map for MG216121
Citations
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