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Cyb5r4 (NM_024195) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216118
Cyb5r4 (tGFP-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $692.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Cyb5r4 or NM_024195"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cyb5r4
Synonyms 2810034J18Rik; B5+B5R; b5/b5r; b5b5r; C79736; Cb5cb5r; Ncb5or
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216118 representing NM_024195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAACGTCCCTTCCCAGGCTTTCCCGGCCCCCGGCTCCCAGCAGCGCGTCTCCTCCCAGGGGCGCA
GCAAGGTACCTTTAAAACAAGGCAGGAGTCTTATGGATTGGATCCGACTGACCAAGAGTGGAAAGGACTT
AACAGGATTGAAGGGCGGGTTAATTGAAGTAACTGAAGAAGAGCTTAAGAAGCACAACAAGAAAGAAGAT
TGTTGGATATGCATAAGAGGGTTTGTTTACAACGTCAGCCCTTATATGGAGTACCATCCGGGTGGGGAGG
ATGAACTCATGAGAGCCGCAGGAGCCGATGGAACAGATCTTTTTAATGAGGTTCATCGTTGGGTCAATTA
TGAATCCATGCTGAAAGAATGCCTGGTTGGCAGGATGGCTGTGAAGCCTGCTGTTCCAAAAGACTGTCAT
GAAGGAAAGAGAGTCTTAAATGGCATGCTTCCCAAGAGCCAAATGTCAGACACACTGCCCAGGGATGTGA
CAGACACACTGCCCAGGGAAGACCTCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCAGTCAC
CATTGTTGTATACACCAAACAAAAGAATATCAGTTTAGACTCAGTAATCGTTGACCTCCAGGATGACTCC
TTGAGAGCAGAAGCAGTCATCAAGGATCACTCTTACCTTGTACATGTTGGGCTAAGCCATGAAGTTCAAG
AAAATTTTTCTGTACGGGTTATTGAAAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAAAGTGT
TTCTTGGCAATGTCTTGGTGATCACCTGGAGAAACATGATTCTTTCATTCCAAAGAAAGATACAGGCTTG
TACTACAGACGGTGCCAGTTAATATCTAAGGAAGATGTCACTCATGACACGAGACTTTTCTGTCTGATGC
TGCCTCCAAGTACTCACCTTCAGGTGCCAGTTGGACAACATGTTTACCTCAAGCTCAGTGTCACAGGTGC
AGAAATAGTAAAGCCATATACACCCGTGTCTGATTCCTTACTCTCAGATTTTAAAGAACCAGTCCTTTCC
CCAAATAAATACATCTGCTTTTTGATCAAAATCTATCCTGCTGGACTCTTCACACCAGAGCTTGATCGTC
TTCAGATTGGAGATTTTATTTCTGTAAGCGGACCTGAAGGCGATTTTAAAGTATCCAAACTCCAGGAAGT
AGAAGATCTCTTTCTATTGGCAGCCGGAACAGGATTCACACCAATGGTCACTGTACTGAATTATGCTTTG
TCTCACATGTCCAGTCTCAGGAAGGTGAAGTTGATGTTCTTCAATAAAACAGAGGACGATATAATTTGGA
GATGTCAGCTAGAGAAGTTGGCATTAAGAGAAAAAAGATTTGATGTTGAATTTGTTCTCTCGGCACCTTC
TCCAGAATGGAATGGCAAACAGGGACACATATCACGAGCTCTTCTCTCTGAATTTTTGCAAAGAAGTTCA
GAAAACTCTAGGGCCTTCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAAGGTTGCTGC
ATGATCTTAACTTTTCTGATGATGAGATTCACGGATTCACCGCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216118 representing NM_024195
Red=Cloning site Green=Tags(s)

MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKED
CWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCH
EGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDS
LRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGL
YYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLS
PNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYAL
SHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSS
ENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024195
ORF Size 1584 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024195.2, NP_077157.2
RefSeq Size 2653 bp
RefSeq ORF 1587 bp
Locus ID 266690
UniProt ID Q3TDX8
Cytogenetics 9 E3.1
Summary NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024195" in other vectors (4)
SKU Description Size Price
MC217526 Cyb5r4 (untagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4), (10ug) 10 ug
$492.00
MR216118 Cyb5r4 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4) 10 ug
$289.00 $492.00
MR216118L3 Lenti ORF clone of Cyb5r4 (Myc-DDK-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4) 10 ug
$792.00
MR216118L4 Lenti ORF clone of Cyb5r4 (mGFP-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4) 10 ug
$792.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.